A5072017321- Bone Tissue Engineering Materials
- Neonatal Respiratory Health Research
- Cystic Fibrosis Research Advances
- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- biodegradable polymer synthesis and properties
- Neurogenesis and neuroplasticity mechanisms
- Pluripotent Stem Cells Research
- Electrospun Nanofibers in Biomedical Applications
- Digestive system and related health
- RNA Interference and Gene Delivery
- Inhalation and Respiratory Drug Delivery
- Virus-based gene therapy research
- Orthopaedic implants and arthroplasty
- Tracheal and airway disorders
- Wireless Body Area Networks
- Pediatric health and respiratory diseases
- Additive Manufacturing and 3D Printing Technologies
- Hydrogels: synthesis, properties, applications
- Dental Implant Techniques and Outcomes
- Silk-based biomaterials and applications
- Graphene and Nanomaterials Applications
- Engineering Technology and Methodologies
Research Centre for Medical Genetics
2016-2025
National Medical Research Center of Dentistry and Maxillofacial Surgery
2020-2024
Sechenov University
2023
Central Research Institute
2020
Research Institute of Medical Genetics of Russian Academy of Medical Sciences
2012-2017
Russian Academy of Sciences
2017
Comet (Russia)
2009-2010
This paper describes an evaluation of the mechanical and biological properties highly porous, biocompatible poly(lactic-co-glycolic acid) (PLGA) scaffolds produced using antisolvent 3D printing technique under various forming conditions. The dependence scaffolds' microstructure, PLGA molecular weight distribution, cell adhesion on temperature injection nozzle diameter was evaluated. All samples consisted fibers with different inner polymer distributions formed by specific radial, porous...
Transplantation of various types stem cells as a possible therapy for stroke has been tested years, and the results are promising. Recent investigations have shown that administration conditioned media obtained after cell cultivation can also be effective in central nervous system pathology (hypothesis their paracrine action). The aim this study was to evaluate therapeutic effects medium hiPSC-derived glial neuronal progenitor rat middle cerebral artery occlusion model ischemic stroke....
In the cohort of Russian patients with cystic fibrosis, p.[Leu467Phe;Phe508del] complex allele (legacy name [L467F;F508del]) CFTR gene is understudied. this research, we present results frequency evaluation [L467F;F508del] in Federation among a F508del/F508del genotype, its effect on clinical course intestinal epithelium ionic channel function, and effectiveness target therapy. The homozygous F508del was determined multiplex ligase-dependent probe amplification followed by polymerase chain...
Adenovirus-mediated gene therapy is a promising tool in bone regenerative medicine. In this work, gene-activated matrices (GAMs) composed of (1) polylactide granules (PLA), which serve as depot for genetic constructs or cell attachment, (2) PRP-based fibrin clot, source growth factors and binding gel, (3) BMP2 providing osteoinductive properties were studied. The study aims to compare the effectiveness vivo ex based on adenoviral with gene, PLA particles, clot defect healing. GAMs Ad-BMP2...
Airway and lung organoids derived from human-induced pluripotent stem cells (hiPSCs) are current models for personalized drug screening, cell–cell interaction studies, disease research. We analyzed the existing differentiation protocols identified optimal conditions obtaining organoids. In this article, we describe a step-by-step protocol differentiating hiPSCs into airway obtained healthy donor five donors with cystic fibrosis. Analysis of cellular composition showed that contain proximal...
Human-induced airway basal cells (hiBCs) derived from human-induced pluripotent stem (hiPSCs) offer a promising cell model for studying lung diseases, regenerative medicine, and developing new gene therapy methods. We analyzed existing differentiation protocols proposed our own protocol obtaining hiBCs, which involves step-by-step of hiPSCs into definitive endoderm, anterior foregut NKX2.1+ progenitors, cultivation on medium with subsequent sorting using the surface marker CD271 (NGFR)....
Gene therapy is one of the most promising approaches in regenerative medicine. Gene-activated matrices provide stable gene expression and production osteogenic proteins situ to stimulate osteogenesis bone repair. In this study, we developed new gene-activated based on polylactide granules (PLA) impregnated with BMP2 polyplexes included chitosan hydrogel or PRP-based fibrin hydrogel. The showed high biocompatibility both vitro mesenchymal stem cells vivo when implanted intramuscularly rats....
Natural and synthetic hydrogel scaffolds containing bioactive components are increasingly used in solving various tissue engineering problems. The encapsulation of DNA-encoding osteogenic growth factors with transfecting agents (e.g., polyplexes) into such scaffold structures is one the promising approaches to delivering corresponding genes area bone defect be replaced, providing prolonged expression required proteins. Herein, a comparative assessment both vitro vivo properties 3D printed...
Gene therapy is one of the most promising approaches in regenerative medicine to restore damaged tissues various types. However, ability control dose bioactive molecules injection site can be challenging. The combination genetic constructs, bioresorbable material, and 3D printing technique help overcome these difficulties not only serve as a microenvironment for cell infiltration but also provide localized gene release more sustainable way induce effective differentiation. Herein,...
Compositions based on chitosan/β-glycerophosphate hydrogels with highly porous polylactide granules can be used to obtain moldable bone graft materials that have osteoinductive and osteoconductive properties. To eliminate the influence of such characteristics as chain length, degree purification, molecular weight a designed material, one-stock chitosan sample was reacetylated degrees deacetylation (DD%) 19.5, 39, 49, 55, 56. A study hydrogel reduced DD% showed low increased MSCs (multipotent...
The presence of pathogenic variants in the CFTR gene causes cystic fibrosis (CF) through various molecular mechanisms that affect formation and functional activity chloride channel. An important factor affecting phenotypic manifestations CF effectiveness targeted therapy is complex alleles with > 2 consecutive mutations per 1 allele, or cis position. influence on has not been sufficiently studied globally due to small number studies. aim study was investigate allele [S466X; R1070Q] a...
The intricate nature of complex alleles presents challenges in the classification CFTR gene mutations, encompassing potential disease-causing, neutral, or treatment-modulating effects. Notably, allele [E217G;G509D] remains absent from international databases, with its pathogenicity yet to be established. Assessing functionality apical membrane ion channels intestinal epithelium employed current measurements (ICM) method, using rectal biopsy material. effectivity CFTR-targeted therapy was...
There is a current clinical need for the development of bone void fillers and bioactive graft substitutes. The use mesenchymal stem cells (MSCs) that are seeded into 3D scaffolds induce generation in event MSCs osteogenic differentiation highly promising. Since calcium ions phosphates promote MSCs, complexes phosphate-containing polymers prospective scaffolds. Calcium poly(ethylene phosphate)s (PEP-Ca) appear to be potentially suitable candidates primarily because PEP’s biodegradability. In...
Cystic fibrosis (CF) is the most common monogenic autosomal recessive disease, associated with pathogenic variants in CFTR gene. The splicing variant c.3140-16T>A (3272-16T>A) has been described previously and, according to Russian CF Patients Registry, occurs a frequency of 0.34%. phenotypic features patients were compared those genotype F508del/F508del. allele had higher average age and at diagnosis, was present greater proportion adults. carrying characterised by better physical...
In dentistry, maxillofacial surgery, traumatology, and orthopedics, there is a need to use osteoplastic materials that have not only osteoinductive osteoconductive properties but are also convenient for use. the study, compositions based on collagen hydrogel were developed. Polylactide granules (PLA) or traditional bone graft, mixture of hydroxyapatite β-tricalcium phosphate (HAP/β-TCP), used gel filling improve mechanical compositions. The tests showed hydrogels filled with 12 wt% highly...
The problem of timely diagnosis and proper management patients with cystic fibrosis is crucial not only in our country, but throughout the world. Experts Union Pediatricians Russia have considered various issues etiology, pathogenesis, epidemiology, diagnosis, treatment this genetic disease a modern light. Particular attention was paid to screening methods for early fibrosis. principles complex therapy were justified, including rational use antibacterial mucolytic drugs enzyme replacement...
The restoration of bone defects resulting from tooth loss, periodontal disease, severe trauma, tumour resection and congenital malformations is a crucial task in dentistry maxillofacial surgery. Growth factor- gene-activated graft substitutes can be used instead traditional materials to solve these problems. New will overcome the low efficacy difficulties associated with use complex situations. One most well-studied active components for morphogenetic protein-2 (BMP-2), which has strong...
The authors of this article analyzed the available literature with results studying prevalence complex alleles CFTR gene among patients cystic fibrosis, and their pathogenicity influence on targeted therapy modulators. Cystic fibrosis (CF) is a multisystemic autosomal recessive disease caused by defect in expression protein, more than 2000 genetic variants are known. Clinically significant divided into seven classes. Information about frequency appears number registers, along traditional...
p.Asn1303Lys (N1303K) is a common missense variant of the CFTR gene, causing cystic fibrosis (CF). In this study, we initially evaluated influence modulators on restoration N1303K-CFTR function using intestinal organoids derived from four CF patients expressing N1303K variant. The forskolin-induced swelling assay in offered valuable insights about beneficial effects VX-770 + VX-661 VX-445 (Elexacaftor Tezacaftor Ivacaftor, ETI) and discouraging prescription VX-809 (Ivacaftor Lumacaftor) or...
Изучение редких вариантов в гене CFTR является актуальным эпоху таргетной терапии CFTR-модуляторами. В данном исследовании изучена клиническая картина муковисцидоза, а также функция CFTR-канала у гомозиготного носителя редкого патогенного варианта c.1329_1350del (p.Asp443GlufsX19), относящегося к I классу нарушений CFTR. Получена полная корреляция результатов функциональных тестов с фенотипической картиной муковисцидоза и потовым тестом. Метод определения разности кишечных потенциалов...