A5031849483- Mesenchymal stem cell research
- Advanced MRI Techniques and Applications
- Neurological Disease Mechanisms and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Neurological Disorders and Treatments
- Cerebrovascular and Carotid Artery Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Acute Ischemic Stroke Management
- Radiomics and Machine Learning in Medical Imaging
- Pluripotent Stem Cells Research
- Advanced Neuroimaging Techniques and Applications
- MRI in cancer diagnosis
- Medical Imaging Techniques and Applications
- Nanoparticle-Based Drug Delivery
- Bone and Joint Diseases
- Tissue Engineering and Regenerative Medicine
- Cardiovascular Health and Disease Prevention
- Brain Tumor Detection and Classification
- Medical Image Segmentation Techniques
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Traumatic Brain Injury and Neurovascular Disturbances
- Cancer Research and Treatments
- Spinal Cord Injury Research
- Medical Imaging and Analysis
Federal Medical-Biological Agency
2020-2025
Pirogov Russian National Research Medical University
2014-2025
Ministry of Health of the Russian Federation
2017-2025
Research Centre for Medical Genetics
2024
Federal Center of Brain Research and Neurotechnologies
2020-2023
Federal State Budgetary Institution "Federal Center For Cardiovascular Surgery" Ministry of Health of The Russian Federation
2019-2022
National Research Tomsk State University
2017
Implantation of reporter-labeled tumor cells in an immunocompetent host involves a risk their immune elimination. We have studied this effect mouse model breast cancer after the orthotopic implantation mammary gland adenocarcinoma 4T1 genetically labelled with luciferase (Luc). Mice were implanted and two derivative Luc-expressing clones 4T1luc2 4T1luc2D6 exhibiting equal vitro growth rates. In vivo, daughter clone exhibited nearly same, 4T1luc2D6, lower rate than parental cells. The...
The middle cerebral artery occlusion (MCAO) model in rats closely imitates ischemic stroke and is widely used. Existing instrumental methods provide a certain level of MCAO guidance, but monitoring the MCA-occluding intraluminal filament position possible complications can be improved. goal this study was to develop MRI-based method simultaneous control position, blood flow intracranial vessels, hemorrhagic complications. Rats were subjected either MRI-guided (group 1, n = 51) or without MRI...
Targeted drug delivery for brain tumor treatment is one of the important objectives in nanomedicine. Human glioblastoma most frequent and aggressive type tumors. The preferential expression membrane protein connexin 43 (Cx43) brain-specific anion transporter (BSAT1) peritumoral area a key component targeted delivery. purpose this study was to design cisplatin-loaded nanogels conjugated with monoclonal antibodies Cx43 BSAT1 intracranial gliomas 101/8. MRI volumetric analysis tumor-bearing...
Background: The creation of a neuroanatomical atlas based on large dataset is fundamentally important work. Creating digital in the era widespread application radiological diagnostic methods both clinical and experimental practice, as well artificial intelligence systems, adds distinct practical aspect to this task. Currently, there no comprehensive rat brain arterial supply, with existing studies relying small animal groups histological descriptions. Therefore, it crucial timely take first...
Cell therapy is an emerging approach to stroke treatment with a potential limit brain damage and enhance its restoration after the acute phase of disease. In this study we tested directly reprogrammed neural precursor cells (drNPC) derived from adult human bone marrow in rat middle cerebral artery occlusion (MCAO) model ischemic using placenta mesenchymal stem (pMSC) as positive control previously confirmed efficacy. Cells were infused into ipsilateral (right) internal carotid male Wistar...
Transplantation of various types stem cells as a possible therapy for stroke has been tested years, and the results are promising. Recent investigations have shown that administration conditioned media obtained after cell cultivation can also be effective in central nervous system pathology (hypothesis their paracrine action). The aim this study was to evaluate therapeutic effects medium hiPSC-derived glial neuronal progenitor rat middle cerebral artery occlusion model ischemic stroke....
Remyelination is a key process enabling post-stroke brain tissue recovery and plasticity. This study aimed to explore the feasibility of demyelination remyelination monitoring in experimental stroke from acute chronic stage using an emerging myelin imaging biomarker, macromolecular proton fraction (MPF). After induction by transient middle cerebral artery occlusion, rats underwent repeated MRI examinations during 85 days after surgery with histological endpoints for animal subgroups on 7th,...
In vivo tracking of transplanted mesenchymal stem cells (MSCs) migration and homing is vital for understanding the mechanisms beneficial effects MSCs transplantation in animal models diseases clinical trials. Transplanted can be labeled with superparamagnetic iron oxide (SPIO) particles visualized using a number sensitive MRI techniques. However, applicability those techniques SPIO-labeled live brain has not been sufficiently investigated. The goal this study was to estimate efficiency...
Systemic transplantation of mesenchymal stem cells (MSCs) is a promising approach for the treatment ischemia-associated disorders, including stroke. However, exact mechanisms underlying its beneficial effects are still debated. In this respect, studies transplanted distribution and homing indispensable. We proposed an MRI protocol which allowed us to estimate dynamic single superparamagnetic iron oxide labeled MSCs in live ischemic rat brain during intravenous after transient middle cerebral...
We performed an in silico, vitro, and vivo assessment of a potassium 2-[2-(2-oxo-4-phenylpyrrolidin-1-yl) acetamido]ethanesulfonate (compound 1) as potential prodrug for cognitive function improvement ischemic brain injury. Using silico methods, we predicted the pharmacological efficacy possible safety rat models. In addition, data showed neuroprotective features compound 1, which were further supported by vitro experiments glutamate excitotoxicity-induced model newborn cortical neuron...
Cell therapy using neural progenitor cells (NPCs) is a promising approach for ischemic stroke treatment according to the results of multiple preclinical studies in animal models. In vast majority conducted studies, therapeutic efficacy NPCs was estimated after intracerebral transplantation, while information effectiveness systemic administration limited. Nowadays, several clinical trials aimed estimate safety and transplantation patients were also conducted. these transplanted...
In vivo optical imaging is widely used in preclinical studies. Recently, the application of systems for visualization gamma-emitting isotopes has become interest since evaluation various organs relies on (99m)Tc-based radiopharmaceuticals (RPs). vitro radioluminescence RPs, including pertechnetate, albumin macroaggregates, dimercaptosuccinic acid, phytate colloid, and ethylenediamine tetramethylene phosphonic was studied with IVIS Spectrum CT™ system. The distribution colloid also without...
Intra-arterial (IA) mesenchymal stem cells (MSCs) transplantation providing targeted cell delivery to brain tissue is a promising approach the treatment of neurological disorders, including stroke. Factors determining distribution after IA administration have not been fully elucidated. Their decoding may contribute improvement technique and facilitate translation stroke therapy into clinical practice. The goal this work was quantitatively assess impact perfusion on transplanted MSCs in rat...
Ischemic stroke triggers a whole cascade of pathological changes in the brain, one which is postischemic inflammation. Since such cases thrombolytic therapy often not possible, methods that modulate inflammation and affect microglia become particularly interesting. We synthesized 3-(2-oxo-4-phenylpyrrolidin-1-yl)propane-1-sulfonate calcium(II) (Compound 4) studied its anti-inflammatory activity vitro vivo models ischemia. Macrophage cell line RAW 264.7 was treated with lipopolysaccharides...
Intra-arterial transplantation of mesenchymal stem cells (MSCs) is an effective delivery route for treatment ischemic brain injury. Despite significant therapeutic effects and targeted to the infraction, serious adverse events such as cerebral embolism have been reported may restrict potential clinical applications this method. In current study, we evaluate complications intra-arterial MSCs administration determine optimum parameters cell transplantation. We injected SPIO-labeled human via...
Disease modifying treatment of spinal cord injury with directly reprogrammed neural precursor cells in non-human primates
Aim. Neural progenitor cells (NPC) are used for the development of cell therapies neurological diseases. Their stereotaxic transplantation in middle cerebral artery occlusion (MCAO) model imitating ischemic stroke results symptom aleviation. However, exploration less invasive options is essential, because a complex procedure and can be applied to humans only by vital indications specialized ward. The aim present study was evaluate efficacy therapy experimental intra-arterial NPC. Materials...