A5091905139- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Terahertz technology and applications
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Electromagnetic Fields and Biological Effects
- Microbial Inactivation Methods
- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- Bone Tissue Engineering Materials
- Neonatal Respiratory Health Research
- Biomedical Ethics and Regulation
- bioluminescence and chemiluminescence research
- Blood properties and coagulation
- biodegradable polymer synthesis and properties
- Neurogenesis and neuroplasticity mechanisms
- Silk-based biomaterials and applications
- Effects of Radiation Exposure
- Angiogenesis and VEGF in Cancer
- Pleural and Pulmonary Diseases
- Congenital heart defects research
- Wound Healing and Treatments
- Cancer Research and Treatments
- Nerve injury and regeneration
- Infrared Thermography in Medicine
Federal Medical-Biological Agency
2015-2024
Sechenov University
2018-2024
Medical Technologies (Czechia)
2022-2024
State Research Institute of Highly Pure Biopreparations
2013-2022
Clinical Hospital No. 8
2018
Medical Radiological Research Center
1999-2017
University of Cincinnati
2010-2012
University of Maryland, Baltimore
2011-2012
University of Baltimore
2012
Sabin Vaccine Institute
2010
Abstract Previous phase I studies demonstrated safety and some beneficial effects of mesenchymal stem cells (MSCs) in patients with mild to moderate idiopathic pulmonary fibrosis (IPF). The aim our study was evaluate the safety, tolerability, efficacy a high cumulative dose bone marrow MSCs rapid progressive course severe IPF. Twenty forced ventilation capacity (FVC) ≥40% diffusing lung for carbon monoxide (DLCO) ≥20% decline both >10% over previous 12 months were randomized into two...
We have designed a novel two-component matrix (SPRPix) for the encapsulation of directly reprogrammed human neural precursor cells (drNPC). The is comprised 1) solid anisotropic complex scaffold prepared by electrospinning mixture recombinant analogues spider dragline silk proteins - spidroin 1 (rS1/9) and 2 (rS2/12) polycaprolactone (PCL) (rSS-PCL), 2) "liquid matrix" based on platelet-rich plasma (PRP). combination PRP promoted drNPC proliferation with formation tissue organoids...
Phytosterols are plant sterols found in foods such as oils, nuts and vegetables. Phytosterols, the same way cholesterol, contain a double bond susceptible to oxidation. The objective of present study was assess potential toxic effects beta-sitosterol oxides on U937 cells. increasing concentrations (0-120 microm) cellular cytotoxicity, apoptosis, antioxidant status genotoxicity assessed over 12, 24 48 h exposure periods. Following 12 h, viability cells treated with 120 microm-beta-sitosterol...
For the first time, data have been obtained on effects of high-intensity terahertz (THz) radiation (with intensity 30 GW/cm 2 , electric field strength 3.5 MV/cm) human skin fibroblasts. A quantitative estimation number histone Н2АХ foci phosphorylation was performed. The per cell studied depending irradiation as well THz pulse energy. performed studies shown that appearance is not related to either oxidative stress (the cells preserve their morphology, cytoskeleton structure, and reactive...
We tested the hypothesis that simultaneous transgenic overexpression of a select quartet growth factors activates diverse signaling pathways for mobilization and participation various stem/progenitor cells cardiogenesis in infarcted heart. Human insulin factor-1 (IGF-1), vascular endothelial factor (VEGF), stromal cell-derived (SDF-1a), hepatocyte (HGF) plasmids were synthesized transfected into skeletal myoblasts (SM) from young male wild-type or rats expressing green fluorescent protein...
The interaction of neural progenitor cells (NPCs) with the extracellular matrix (ECM) plays an important role in tissue regeneration. Understanding which motifs ECM proteins are crucial for normal NPC adhesion, proliferation, and differentiation is order to create more adequate engineered models efficiently study central nervous system regeneration mechanisms. We have shown earlier that anisotropic matrices prepared from a mixture recombinant dragline silk proteins, such as spidroin 1 2, by...
Recruitment and retention of circulating progenitor cells at the site injured or ischemic tissues facilitates adult neo-vascularization. We hypothesized that cell therapy could modulate local neo-vascularization through vascular endothelial growth factor (VEGF)/stromal cell-derived factor-1 (SDF-1) axis by paracrine effects on cells. isolated from rat bone marrow a subset multipotent (MDPC). In vitro, MDPCs secreted multiple cytokines related to inflammation angiogenesis, including monocyte...
Objective— In vitro, transglutaminase-2 (TG2)–mediated activation of the β-catenin signaling pathway is central in warfarin-induced calcification, warranting inquiry into importance this axis as a target for preventive therapy vascular calcification vivo. Methods and Results— The adverse effects elastocalcinosis rat model include aortic media, loss cellular component vessel wall, isolated systolic hypertension, associated with accumulation TG2 signaling. These warfarin can be completely...
We evaluated the applicability of chitosan-g-oligo(L,L-lactide) copolymer (CLC) hydrogel for central nervous system tissue engineering. The biomechanical properties CLC were characterized and its biocompatibility was assessed with neural progenitor cells obtained from two different sources: H9-derived stem (H9D-NSCs) directly reprogrammed precursor (drNPCs). Our study found that optically transparent possessed characteristics suitable culturing human stem/precursor noncytotoxic. When seeded...
Donor-recipient cell interactions are essential for functional engraftment after nonautologous transplantation. During this process, transplant is characterized and defined by between transplanted cells with local recruited inflammatory cells. The outcome of these determines donor fate. Here, we provide evidence that lineage-committed embryonic stem (ESC)-derived vascular progenitor the target major histocompatibility complex (MHC) class I-dependent, natural killer (NK) cell-mediated...
THz radiation induces a variety of processes in cells and has attracted the attention researchers recent decades. Here, data on effects high-intensity terahertz (THz) human directly reprogrammed neural progenitor (drNPCs) neuroblastoma (SK-N-BE (2)) were obtained for first time. The results demonstrated that exposure non-tumor tumor to broadband (0.1-3 THz) pulses with intensity 21 GW/cm
A review of literature considers the problem hyaline cartilage biology and repair following by injury, surgical defects. Repair techniques based on direct substitution (mosaicplasty, osteochondral allotransplantation, minced autotransplantation in gel), bone morrow stimulation (abrasion, drilling, microfracture, matrix-induced chondrogenesis) were characterized terms clinics. Most attention was addressed to cell technology tissue engineering.
Abstract Exposure of cells or biological tissues to high-power pulses terahertz (THz) radiation leads changes in a variety intracellular processes. However, the role heating effects due strong absorption THz by water molecules still stays unclear. In this study, we performed numerical modelling order estimate thermal impact on single pulse as well series pulses. A finite-element (FE) model that provides solutions for heat conduction equation is employed compute temperature increase. simple...