A5091709490- Venomous Animal Envenomation and Studies
- Biochemical and Structural Characterization
- Rabies epidemiology and control
- Marine Invertebrate Physiology and Ecology
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Antimicrobial Peptides and Activities
- Cytomegalovirus and herpesvirus research
- Biotin and Related Studies
- Cell Adhesion Molecules Research
- Ion channel regulation and function
- CAR-T cell therapy research
- Neurological Complications and Syndromes
- bioluminescence and chemiluminescence research
- Lysosomal Storage Disorders Research
- TGF-β signaling in diseases
- Bacillus and Francisella bacterial research
- Inflammasome and immune disorders
- Herpesvirus Infections and Treatments
- Genomics and Chromatin Dynamics
- Sphingolipid Metabolism and Signaling
- Orthopaedic implants and arthroplasty
- Vector-borne infectious diseases
- Invertebrate Immune Response Mechanisms
- Biochemical Analysis and Sensing Techniques
Instituto Butantan
2010-2022
Instituto de Pesquisas Energéticas e Nucleares
2021
Weatherford College
2021
Universidade Estadual de Campinas (UNICAMP)
2012-2013
Universidade de São Paulo
2002
Universidade Brasil
2002
Lectins are glycan-binding receptors that recognize glycan epitopes on foreign pathogens and in the host systems. They can be involved functions include innate immunity, development, immune regulation homeostasis. Several lectins have been purified characterized from fish species. In this work, using cation-exchange chromatography, a galactose-specific lectin belonging to family of C-type was isolated venom Brazilian venomous Thalassophryne nattereri. Nattectin is basic, non-glycosilated, 15...
SVMPs are multi-domain proteolytic enzymes in which disintegrin-like and cysteine-rich domains bind to cell receptors, plasma or ECM proteins. We have recently reported that jararhagin, a P-III class SVMP, binds collagen with high affinity through an epitope located within the Da-disintegrin sub-domain. In this study, we evaluated binding of jararhagin alpha(2)beta(1) integrin (collagen receptor) using monoclonal antibodies recombinant fragments. solid phase assays, was detectable from...
Snake venom metalloproteinases (SVMPs) are widely distributed in snake venoms and versatile toxins, targeting many important elements involved hemostasis, such as basement membrane proteins, clotting platelets, endothelial inflammatory cells. The functional diversity of SVMPs is part due to the structural organization different combinations catalytic, disintegrin, disintegrin-like cysteine-rich domains, which categorizes 3 classes precursor molecules (PI, PII PIII) further divided 11...
Snake venom metalloproteinases (SVMPs) are zinc-dependent enzymes responsible for most symptoms of human envenoming. Like matrix (MMPs) and a disintegrin metalloproteinase (ADAM) proteins, SVMPs synthesized as zymogens, enzyme activation is regulated by hydrolysis their prodomain, but the processing still unclear. In this study, we attempted to identify presence prodomain in different compartments snake glands zymogens or free form elucidate some mechanism involved SVMP activation. Using...
Gyroxin is one of main serine proteases Crotalus durissus terrificus venom, representing about 2% the protein content in crude venom. It a 33 kDa glycoprotein with 3.8% by weight sugar moiety. This toxin induces hemotoxicity mice and neurological condition called barrel rotation syndrome. In present work, we report molecular cloning five new nucleotide sequences from cDNA library venom glands single specimen C. d. terrificus. These have been analyzed silico respect to their organization...
Snake venom metalloproteinases (SVMP) are abundant toxins in venoms of viper snakes and play a relevant role the complex multifactorial tissue damage characteristic Viperidae envenoming. Jararhagin, SVMP isolated from Bothrops jararaca venom, induces fast onset hemorrhagic lesions acting directly on capillary vessels, which disrupted by toxin adhesion degradation extracellular matrix proteins like collagen IV. Jararhagin also triggers inflammatory response, where endothelial cells activated,...
Human accidents with spiders of the genus Loxosceles are an important health problem affecting thousands people worldwide. Patients evolve to severe local injuries and, in many cases, systemic disturbances as acute renal failure, which cases antivenoms considered be most effective treatment. However, for antivenom production, extraction venom used immunization process is laborious and yield very low. Thus, groups have been exploring use recombinant toxins, particularly phospholipases D...
Abstract Commercially available preparations of methionyl-human BMP-2 and CHO-derived hBMP-2, which belongs to the transforming growth factor β (TGF-β) superfamily, were used for a complete characterization. This protein is an extremely efficient osteoinductor that plays important role during bone regeneration embryonic development. Characterization was carried out via SDS-PAGE Western blotting, followed by reversed-phase HPLC, size-exclusion HPLC MALDI-TOF-MS. The classical in vitro...
Brown spider envenomation results in dermonecrosis, intravascular coagulation, haemolysis and renal failure, mainly owing to the action of sphingomyelinases D (SMases D), which catalyze hydrolysis sphingomyelin produce ceramide 1-phosphate choline or lysophosphatidylcholine lysophosphatidic acid. Here, heterologous expression, purification, crystallization preliminary X-ray diffraction analysis LgRec1, a novel SMase from Loxosceles gaucho venom, are reported. The crystals belonged space...
We identified Pycard and BC017158 genes as putative effectors of the Quantitative Trait locus (QTL) that we mapped at distal chromosome 7 named Irm1 for Inflammatory response modulator 1, controlling acute inflammatory (AIR) production IL-1β, dependent on activation NLRP3 inflammasome. obtained mapping through genome-wide linkage analysis Single Nucleotide Polymorphisms (SNPs) in a cross between High (AIRmax) Low (AIRmin) responder mouse lines produced by several generations bidirectional...
Treatment of cancer using gene therapy is based on adding a property to the cell leading its elimination. One possibility use suicide genes that code for enzymes transform pro-drug into cytotoxic product. The most extensively used herpes simplex virus thymidine kinase (TK) gene, followed by administration antiviral drug ganciclovir (GCV). choice promoter drive transcription transgene one determinants given transfer vector usefulness, as different promoters show efficiencies depending target...