A5101654027- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Viral Infections and Immunology Research
- Microbial infections and disease research
- Parasite Biology and Host Interactions
- Aquaculture disease management and microbiota
- Mosquito-borne diseases and control
- Herpesvirus Infections and Treatments
- Rabies epidemiology and control
- Plant Pathogens and Fungal Diseases
- Invertebrate Immune Response Mechanisms
- Bacteriophages and microbial interactions
- Renal Diseases and Glomerulopathies
- Congenital heart defects research
- Plant-Microbe Interactions and Immunity
- Streptococcal Infections and Treatments
- Plant and fungal interactions
- T-cell and Retrovirus Studies
- Identification and Quantification in Food
- Animal Genetics and Reproduction
- Ocean Acidification Effects and Responses
Sichuan Animal Science Academy
2017-2024
Hunan University of Science and Technology
2024
Tianjin University of Traditional Chinese Medicine
2024
Sichuan Agricultural University
2016-2022
Dalian Ocean University
2010-2022
Sichuan Entry-Exit Inspection and Quarantine Bureau
2017
China Agricultural University
1998
Recombination among porcine reproductive and respiratory syndrome viruses (PRRSVs), coupled with point mutations, insertions, deletions occurring in the genome, is considered to contribute emergence of new variants. Here, we report complete genome sequences a PRRSV field strain, designated SCN17, isolated from RespPRRS MLV-vaccinated piglet China 2017. Sequence alignment revealed that SCN17 had discontinuous 131-amino acid (111 + 1 19-aa) deletion NSP2-coding region identical NADC30 when...
The NADC34-like porcine reproductive and respiratory syndrome virus 2 (PRRSV-2) first emerged in China 2017 has the potential to become dominant PRRSV strain China. Here, a novel PRRSV-2, SCcd2020, was isolated from diseased piglets Sichuan province, southwest 2020. complete viral genome determined analyzed. An ORF5-based phylogenetic analysis showed that SCcd2020 clustered with strains, whereas sequence isolate NADC30-like viruses it contains discontinuous 131-aa deletion NSP2 when compared...
Abstract African swine fever is an acute, febrile and highly virulent porcine disease causing serious economic losses worldwide. The pK205R protein of the virus (ASFV) largely expressed in early stages infection, which has given K205R gene extensive attention. In this study, ASFV was cloned Escherichia coli BL21 (DE3). Expression histidine-tagged with a molecular mass 44 kDa determined by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) Western blot analysis....
Rabbit hemorrhagic disease (RHD) is an acute fatal caused by the rabbit virus (RHDV). Since first outbreaks of type 2 RHDV (RHDV2) in April 2020 China, persistence this population has substantial economic losses husbandry. Previous failures preventing RHDV2 prompted us to further investigate immune mechanisms underlying virus’s pathogenicity, particularly concerning spleen, a vital component mononuclear phagocyte system (MPS). For this, previous isolate, CHN/SC2020, was utilized challenge...
Infectious laryngotracheitis disease is an acute, highly contagious viral seriously affecting poultry industry worldwide. In this study, a rapid and simple immune colloidal gold test strip for detecting infectious virus (ILTV) was developed based on membrane chromatography with monoclonal antibodies (mAbs) against gJ protein of ILTV systematically evaluated the detection from clinical samples. mAb 2D4 1D7 conjugated as detector antibody strip. Another mAb, 1D8 1G3, used capture complex at...
The rabbit hemorrhagic disease virus 2 (RHDV2 or GI.2) is a highly contagious agent leading to lethal in rabbits. It frequently recombines with other Lagovirus genus, generating epidemical variants high pathogenicity. In this study, twenty-two liver samples tested positive for GI.2 VP60 gene, were collected farms from several geographical regions China. All specimens submitted RT-PCR detection, nucleotide sequencing and phylogenetic analysis. addition, suspected recombinants evaluated...
We examined 220 Pacific oysters Crassostrea gigas obtained from 11 locations along China's coasts for the presence of 2 protistan parasites Haplosporidium nelsoni (MSX; multinucleated sphere X) and H. costale (SSO; seaside organism). Haplosporidium-like plasmodia were histologically observed in 9 (4.09%) 7 locations. Five had mixed infections, 4 infected only with as determined by situ hybridization (ISH) polymerase chain reaction (PCR). This is first report infection bivalves Chinese waters.
A loop-mediated isothermal amplification (LAMP) assay was developed for rapid, sensitive and specific detection of African swine fever virus (ASFV). set LAMP primers designed based on the sequence ASFV gene K205R. Reaction temperature time were optimized to 64 oC 60 min, respectively. products detected by agarose gel electrophoresis or visually with addition fluorescent dye. The limit approximately 6 copies target per microliter, 100 times more than conventional PCR. is a simple inexpensive...
Here we aimed to develop a capillary electrophoresis-based high-throughput multiplex polymerase chain reaction (PCR) system for the simultaneous detection of nine pathogens in swine. Nine pairs specific primers and set universal were designed; PCR was established. The specificity cross-reactivity this assay examined, limit determined using serial 10-fold dilutions plasmids containing target sequences. further tested 144 clinical samples. We found that amplification peaks observed, had high...
This study was aimed to establish a highly specific and sensitive loop-mediated isothermal amplification (LAMP) method for diagnosing avian infectious laryngotracheitis (AILT). DNA extracted from isolated virus (ILTV) strains control samples, followed by PCR using three sets of six primers. The detection efficiency the LAMP assay evaluated turbidity calcein methods. sensitivity then assessed concentration gradient specificity analysis. Furthermore, compared. Finally, clinical test performed...
Porcine circovirus 4 (PCV4) was first discovered in 2019 a herd of pigs with porcine respiratory disease, dermatitis and nephropathy syndrome Hunan Province, China. It has subsequently been detected other provinces South Korea. In consideration the potential virus to cause an epidemic, rapid, sensitive, specific detection PCV4 is needed, as facilitation further epidemiological research through elucidation whole genome PCV4. This study had those two aims.Fifty-five blood samples, pig tissue...
Background: MN and IgAN pose challenges in clinical treatment with existing therapies primarily focusing on symptom relief often yielding unsatisfactory outcomes. The search for novel drug targets remains crucial to address the shortcomings managing both kidney diseases. Methods: Utilizing GWAS data, instrumental variables plasma proteins were derived, various analyses, including bidirectional Mendelian randomization Bayesian co-localization, conducted explore reverse causal relationships...
To investigate the genetic variation in Haemophilus parasuis (HPS) Sichuan, China, 11 isolates were analyzed based on outer membrane protein P2 (OMPP2) sequence. Sequence analysis showed that shared 93.0 to 100% nucleotide homology with 15 reference strains, and consistency between 26 strains was 89.0%. The of HPS-1, 2, 4, 5, 6, 7, 8, 10 had a 69-base deletion from 770 base pairs (bp) 850 bp, which infrequent China. phylogenetic tree HPS-3 HPS-8 closer relationships European Japanese but...
The Palaemonetes sinensis aquaculture industry in Panjin City, Liaoning Province, China, experienced heavy losses October 2018. Morbidity of cultured shrimp reached 50% and was characterized by cloudiness muscle the gradual spread disease within population. When infection mild, histopathological examinations revealed that cells contained a considerable number microorganisms. In extreme cases, structure hepatopancreatic glandular fiber obscured or even vanished. Electron microscope...