A5103132285- Microbial infections and disease research
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Streptococcal Infections and Treatments
- Genomics and Phylogenetic Studies
- Animal Nutrition and Health
- Gut microbiota and health
- Reproductive Physiology in Livestock
- Ruminant Nutrition and Digestive Physiology
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Effects of Environmental Stressors on Livestock
- Energy, Environment, and Transportation Policies
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Energy, Environment, Economic Growth
- Mycobacterium research and diagnosis
- interferon and immune responses
- Milk Quality and Mastitis in Dairy Cows
- Animal Disease Management and Epidemiology
- Mosquito-borne diseases and control
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Ginseng Biological Effects and Applications
Sichuan Agricultural University
2009-2024
Heilongjiang Institute of Technology
2009
Xinjiang Medical University
2009
Metabolic maladaptation in dairy cows after calving can lead to long-term elevation of ketones, such as β-hydroxybutyrate (BHB), representing the condition known hyperketonemia, which greatly influences health and production performance during lactation period. Although gut microbiota is alter with association microbial metabolites development hyperketonemia remains unknown. In this study, we performed a multi-omics analysis investigate associations between fecal community, fecal/plasma...
(1) Background: Short-read sequencing allows for the rapid and accurate analysis of whole bacterial genome but does not usually enable complete assembly. Long-read greatly assists with resolution complex genomes, particularly when combined short-read Illumina data. However, it is clear how different assembly strategies affect genomic accuracy, completeness, protein prediction. (2) Methods: we compare Haemophilus parasuis, which causes Glässer’s disease, characterized by fibrinous...
Bovine mastitis is a common disease occurring in dairy farms and can be caused by more than 150 species of pathogenic bacteria. One the most causative organisms Streptococcus agalactiae , which also potentially harmful to humans aquatic animals. At present, research on S . China mostly concentrated northern region, with limited southeastern southwestern regions. In this study, total 313 clinical samples from large-scale five regions Sichuan were collected for isolation The epidemiological...
With the improper use of antibiotics, an increasing number multidrug-resistant bacteria have been reported worldwide, posing challenges for disease treatment. Klebsiella pneumoniae is important zoonotic pathogen that colonises respiratory tract. Endolysin therapy has emerged with development phages. In this study, a lytic phage vB_KpnS_MK54 was isolated from drinking water forest musk deer (FMD) farm in Sichuan Province. It first obtained FMD. The primary biological characteristics were...
Streptococcus suis serotype 2 (SS2) is a Gram-positive bacterium. It common and significant pathogen in pigs cause of zoonotic meningitis humans. can lead to sepsis, endocarditis, arthritis, pneumonia. If not diagnosed treated promptly, it has high mortality rate. The pan-genome SS2 open, with an increasing number genes, the core genome accessory may exhibit more pronounced differences. Due diversity SS2, genes related its virulence resistance are still unclear. In this study, strain was...
Neurotropic viruses severely damage the central nervous system (CNS) and human health. Common neurotropic include rabies virus (RABV), Zika virus, poliovirus. When treating infection, obstruction of blood-brain barrier (BBB) reduces efficiency drug delivery to CNS. An efficient intracerebral can significantly increase facilitate antiviral therapy. In this study, a glycopeptide (RVG) functionalized mesoporous silica nanoparticle (MSN) packaging favipiravir (T-705) was developed generate...
Left displaced abomasum (LDA) in postpartum dairy cows contributes to significant economic losses. Dairy with LDA undergo excessive lipid mobilization and insulin resistance. Although gut dysbiosis is implicated, little known about the role of microbiota abnormal metabolic processes LDA. To investigate functional links among microbiota, metabolites, disease phenotypes LDA, we performed 16S rDNA gene amplicon sequencing liquid chromatography-tandem mass spectrometry (LC-MS/MS) fecal samples from (
Haemophilus parasuis (H. parasuis) is a conditional pathogen with the ability to form biofilms which can lead ineffective drug treatment and severe chronic infections resulting in significant economic losses pig industry. Currently, knowledge of biofilm formation by H. not well developed. The objective this study was investigate three-dimensional morphology perform transcriptomic analysis on cells versus planktonic forms. results showed that proteins DNA accounted for large proportion...
Glaesserella parasuis is a gram-negative bacterium that causes fibrotic polyserositis and arthritis in pig, significantly affecting the pig industry. The pan-genome of G. open. As number genes increases, core accessory genomes may show more pronounced differences. associated with virulence biofilm formation are also still unclear due to diversity . Therefore, we have applied pan-genome-wide association study (Pan-GWAS) 121 strains Our analysis revealed genome consists 1,133 cytoskeleton,...
Abstract Bovine mammary epithelial cells (bMECs) are the main of dairy cow gland. In addition to their role in milk production, they effector immunity. However, there is little information about changes metabolites bMECs when stimulated by lipopolysaccharide (LPS). This study describes a metabolomics analysis LPS-stimulated provide basis for identification potential diagnostic screening biomarkers and possible treatments bovine gland inflammation. present study, were challenged with 500...
Prepartum exercise (PA) has been proposed as a strategy for the peripartum management of dairy cows; however, mechanism by which PA affects metabolism not elucidated. Here, we investigated metabolic changes in transition cows with PA. Holstein multiparous were assigned to an (n = 12) or control group; group walked targeted 45 min at 3.25 km/h, two times day. Plasma non-esterified fatty acid (NEFA), β-hydroxybutyric (BHBA), glucose, and triglyceride levels measured, profiles analyzed using...
The present study aims to evaluate the alterations in gut microbiome and plasma metabolites of dairy calves with β-glucan (BG) supplementation. Fourteen healthy newborn similar body weight were randomly divided into control (
Background Recent studies have characterized that microRNA (miRNA) is a suitable candidate for the study of bleomycin/LPS-induced pulmonary fibrosis, but knowledge on miRNA in bacteria-induced fibrosis (BIPF) limited. Forest musk deer ( Moschus berezovskii , FMD) an important endangered species has been seriously affected by BIPF. We sought to determine whether exist modulates pathogenesis BIPF FMD. Methods High-throughput sequencing and RT-qPCR were used differentially expressed miRNAs...
Glässer's disease, caused by Haemophilus parasuis (HPS), is responsible for economic losses in the pig industry worldwide. However, existing commercial vaccines offer poor protection and there are significant barriers to development of effective vaccines.In current study, we aimed identify potential vaccine candidates design a multi-epitope against HPS performing pan-genomic analysis 121 strains using reverse vaccinology approach.The designed constructs consist predicted epitopes B T cells...
Cecropin P1, acting as an antimicrobial, has a broad-spectrum antibacterial activity with some antiviral and antifungal properties. It is promising natural alternative to antibiotics which originally isolated from the pig intestinal parasitic nematode Ascaris suum. Many studies have shown that P1 helpful for prevention or treatment of clinical diseases. Therefore, it very necessary establish safe, nontoxic, efficient expression method P1. The results indicated recombinant protein was about...
Klebsiella pneumoniae is an important pathogen that can infect both humans and cattle. The widespread K. its high drug resistance make it difficult to treat infections/diseases. In this study, a lytic bacteriophage vB_Kpn_B01 was isolated from dairy farm trough in Sichuan Province, biological properties were studied, the entire genome of sequenced. therapeutic effects phage on disease-causing mice preliminarily tested. Phages found study are double-stranded DNA bacterial viruses belonging...
Klebsiella pneumoniae is an important pathogen commonly associated with opportunistic infections. In this study, lung pathogenic K. (LPKP) was isolated and identified from suppurative in forest musk deer by conventional methods 16S ribosomal RNA sequence analysis. Median lethal dose histopathologic analysis were used to demonstrate pathogenicity of the organism mice. Furthermore, a draft genome LPKP sequenced, its virulence genes detected. One hundred twenty-two encoded determinant capsule...
Quantum dots (QDs) have a promising prospect in live-cell imaging and sensing because of unique fluorescence features. QDs aroused significant interest the bio-imaging field through integrating properties delivery function biomaterial. The natural tropism Canine Parvovirus (CPV) to transferrin receptor can target specific cells increase targeting ability cell imaging. CPV virus-like particles (VLPs) from expression CPV-VP2 capsid protein prokaryotic system were examined encapsulate deliver...
Based on the annual data from 1980 to 2014, path analysis model was employed investigate influence of gross domestic product (GDP), total population (TP), and net export value (NEV) energy consumption (TEC) in China. The results showed that (1) GDP, influenced 59.73% TEC, played most important role affect TEC. Meanwhile, 37.69% TEC directly determined by 22.04% indirectly GDP through TP NEV. (2) secondary factor TEC; 6.02% TP, 15.20% affected TP. Moreover, 21.22% associated with (3) NEV had...