A5008804083- Animal Disease Management and Epidemiology
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Advanced biosensing and bioanalysis techniques
- Viral Infections and Immunology Research
- Herpesvirus Infections and Treatments
- Poxvirus research and outbreaks
- Molecular Biology Techniques and Applications
- Viral Infections and Vectors
- Bacteriophages and microbial interactions
- Mosquito-borne diseases and control
- Reproductive tract infections research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Vector-Borne Animal Diseases
- Cervical Cancer and HPV Research
- Plant Virus Research Studies
- Microbial infections and disease research
- T-cell and Retrovirus Studies
- Sperm and Testicular Function
- Salmonella and Campylobacter epidemiology
- Bacterial Identification and Susceptibility Testing
- Advanced Nanomaterials in Catalysis
- Reproductive Physiology in Livestock
- Mycotoxins in Agriculture and Food
Shanghai Customs College
2020-2023
Chongqing University
2011-2021
Entry Exit Inspection and Quarantine Bureau
2012-2016
Lumpy skin disease (LSD) is a devastating viral that occurs in cattle. In China, it was first detected the Xin-Jiang autonomous region, near border with Kazakhstan, August 2019. As there were no new occurrences of LSD either country following detection, initial introduction virus remains unknown. Arthropod vectors considered as potential vectors. Consequently, to identify arthropod involved transmitting (LSDV), an insect surveillance campaign launched at four different sites scattered along...
Lumpy skin disease virus (LSDV) is a severe and highly contagious form of cowpox. As LSDV continues to mutate there no vaccine treatment in nonendemic countries, early detection becomes an important basis for epidemic prevention control, especially conserved sequences. A new label-free sensitive fluorescence method was developed based on light-up RNA aptamer detecting LSDV. The integrated recombinase polymerase amplification (RPA), CRISPR/Cas12a, 10–23 DNAzyme, Baby Spinach triple cascade...
Experimentally, Cas12a can recognize multiple protospacer adjacent motif (PAM) sequences and is not restricted to the “TTTN”. However, application of CRISPR/Cas12a system still limited by PAM for double-stranded DNA (dsDNA). Here, we developed asymmetric RPA (Asy-RPA) completely break limitations PAM. Asy-RPA only achieved efficient amplification but also converted dsDNA single-stranded (ssDNA) without complicated steps. The ssDNA products activated trans-cleavage activity Cas12a, outputting...
Simple, rapid, and highly sensitive methods for single-stranded nucleic acid detection are of great significance in clinical testing. Meanwhile, common inseparable from the participation enzymes, which greatly increases their complexity. Herein, an enzyme-free method combining HCR CHA is established to detect acid. A target induces auxiliary hairpin strands open secondary structure, exposing partial sequences that can trigger catalytic assembly (CHA) hybridization chain reactions (HCR),...
The samples were difficult to accurately determine positive or negative between 35 and 40 cycles by real-time quantitative PCR (qPCR) as the standard method. Here, we developed one-tube nested recombinase polymerase amplification (ONRPA) technology with CRISPR/Cas12a overcome this difficulty. ONRPA broke plateau substantially enhance signals, which considerably improved sensitivity eliminated problem of gray area. Using two pairs primers one after another, it precision lowering probability...
BACKGROUND Chlamydiae are spread globally and cause infectious diseases in both humans animals. The existing detection methods for this disease have numerous shortcomings, including low sensitivity, time consuming procedures, high contamination vulnerability. MATERIAL AND METHODS To overcome shortcomings detecting animal chlamydiosis, a multiplex quantitative polymerase chain reaction (PCR) assay was established simultaneously differentiating 3 Chlamydia species (C. pecorum, C. abortus,...
Porcine chlamydial infection is an enzootic infectious disease caused by multiple members of the family Chlamydiaceae (e.g. Chlamydophila abortus, Chlamydia suis, and pneumoniae). Rapid accurate differentiation these pathogens critical in control prevention disease. The aim current study was to develop a nested multiplex polymerase chain reaction (nmPCR) assay simultaneously detect 3 clinical samples. In first round nmPCR, 1 pair family-specific primers were used amplify 1,100 base (bp)...
Peste des petits ruminants (PPR) is a serious acute, highly contagious disease caused by the peste virus (PPRV). This study aims to establish qRT-PCR assay with an internal amplification control for rapid and accurate detection of PPRV. The primers probes PPRV N were based on national standard diagnostic techniques PPR China, pair