A5102946452- CRISPR and Genetic Engineering
- Computational Drug Discovery Methods
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Graph theory and applications
- Epigenetics and DNA Methylation
- Connective tissue disorders research
- Immune Response and Inflammation
- SARS-CoV-2 and COVID-19 Research
- Ferroptosis and cancer prognosis
- Cancer-related molecular mechanisms research
- Mosquito-borne diseases and control
- Cancer, Hypoxia, and Metabolism
- Pluripotent Stem Cells Research
- Animal Disease Management and Epidemiology
- Free Radicals and Antioxidants
- Bacteriophages and microbial interactions
- Antimicrobial Peptides and Activities
- Viral gastroenteritis research and epidemiology
- COVID-19 impact on air quality
- Cancer-related gene regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetic factors in colorectal cancer
- NF-κB Signaling Pathways
Xinxiang Medical University
2025
First Affiliated Hospital of Xinxiang Medical University
2025
Hainan University
2023-2024
Chaohu University
2022-2023
Prince of Wales Hospital
2021-2022
Hubei University
2020-2022
State Council of the People's Republic of China
2022
Chinese University of Hong Kong
2021-2022
Nanchang University
2021
Third Affiliated Hospital of Nanchang University
2021
Abstract Argonaute (Ago) proteins are conserved nucleic acid-guided present in all domains of life. Eukaryotic (eAgos) key players RNA interference pathways and function as RNA-guided endonucleases at physiological temperatures. Although eAgos considered to evolve from prokaryotic (pAgos), previously studied pAgos were unable catalyze cleavage Here, we describe a distinctive pAgo mesophilic bacteria Kurthia massiliensis (KmAgo). KmAgo utilizes DNA guides cleave single-stranded (ssDNA)...
Abstract Aliphatic α,ω‐dicarboxylic acids (DCAs) are a class of useful chemicals that currently produced by energy-intensive, multistage chemical oxidations hazardous to the environment. Therefore, development environmentally friendly, safe, neutral routes DCAs is important. We report an in vivo artificially designed biocatalytic cascade process for biotransformation cycloalkanes DCAs. To reduce protein expression burden and redox constraints caused multi-enzyme single microbe, pathway...
Escherichia coli is a major environmental pathogen causing bovine mastitis, which leads to mammary tissue damage and cell death. We explored the effects of probiotic Lactobacillus rhamnosus GR-1 on ameliorating E. coli-induced inflammation in primary epithelial cells (BMECs). Increased Toll-like receptor 4 (TLR4), NOD1, NOD2 mRNA expression was observed following challenge, but this increase attenuated by L. pretreatment. Immunofluorescence Western blot analyses revealed that pretreatment...
Argonaute proteins are widespread in prokaryotes and eukaryotes with diversified catalytic activities. Here, we describe an from Marinitoga hydrogenitolerans (MhAgo) all eight cleavage Utilization of four types guides efficient single-stranded DNA (ssDNA) RNA targets revealed. The preference for the 5′-terminus nucleotides 5′P guides, but no obvious preferences that 5′OH is further uncovered. Moreover, efficiency heavily impaired by mismatches central 3′-supplementary regions affinity...
The purpose of this study was to explore the film-forming properties cinnamon essential oil (CEO) and chitosan (CS) effect their composite coating on postharvest apple diseases. results demonstrated that exhibits favorable at CEO concentrations below 4% (v/v). effectiveness in disease control can be attributed two factors: direct inhibitory activity against pathogens vitro induced resistance triggered by CS fruits. Importantly, incorporation did not interfere with induction harvested apples....
LIN28 is an evolutionarily conserved RNA-binding protein with critical functions in developmental timing and cancer. However, the molecular mechanisms underlying LIN28's oncogenic properties are yet to be described. RNA-protein immunoprecipitation coupled genome-wide sequencing (RIP-Seq) analysis revealed significant binding within 843 mRNAs breast cancer cells. Many of LIN28-bound implicated regulation RNA cell metabolism. We identify heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1), a...
A simple and user-friendly nucleic acid sensing platform with 10 aM sensitivity, named USPCRP (combines ultrashort PCR Pyrococcus furiosus Argonaute cleavage for nuleic acids detection) is reported. The product of this could be directly used as a DNA guide to mediate PfAgo molecular beacons.
A topological index (TI) is a real number that defines the relationship between chemical structure and its properties remains invariant under graph isomorphism. TIs defined for structures are capable of predicting physical properties, reactivity biological activity. Several kinds have been studied different molecular structures. Graphene thinnest material known to man also extremely strong while being good conductor heat electricity. With such unique features, graphene derivatives found...
Neuroinflammation is closely implicated in the pathogenesis of neurological diseases. The hallmark neuroinflammation microglia activation. Upon activation, are capable producing various proinflammatory factors and accumulation these contribute to neuronal damage. Therefore, inhibition microglia-mediated might hold potential therapy for disorders. 2,3,5,4′-Tetrahydroxystilbene-2-O- β -D-glucoside (TSG), an active component extracted from Polygonum multiflorum , reported be beneficial human...
Patients with cardiopulmonary failure may not be fully supported typical configurations of extracorporeal membrane oxygenation (ECMO), either veno-arterial (VA) or veno-venous (VV). Veno-arterial-venous (VAV)-ECMO is a technique used to support the systems during periods inadequate gas exchange and perfusion. In severe case coronavirus disease 2019 (COVID-19), which simultaneously affects heart lung, VAV-ECMO improve patient’s recovery potential. We report 72-year-old woman acute respiratory...
Transcriptomic analysis of racially diverse fibroblasts and iPSCs shows that individual variability affects pluripotent potential.
Abstract The Multi‐Threat Medical Countermeasure (MTMC) technique is crucial for developing common biochemical signaling pathways, molecular mediators, and cellular processes. This study revealed that the Nod‐like receptor 3 (NLRP3) inflammasome pathway may be a significant contributor to cytotoxicity induced by various organophosphorus pesticides (OPPs). demonstrated exposure six different types of OPPs (paraoxon, dichlorvos, fenthion, dipterex, dibrom, dimethoate) led in BV2 cells, which...
We report the antimicrobial resistance of 191 fish and 61 pork Group B Streptococcus (GBS) procured from Hong Kong wet markets. Two-hundred-and-fifty-two GBS strains were isolated 992 freshwater 361 pig offal during 2016-2019. The homogenised samples plated on selective media, followed by identification through MALDI-TOF-MS. Molecular characterisation, an antibiotic susceptibility test, biofilm formation performed strains. isolation rates 19.3% (191 fish) 16.9% (61 organs), respectively. was...
The continuous and rapid surge of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants with high transmissibility evading neutralization is alarming, necessitating expeditious detection the concerned. Here, we report development SARS-CoV-2 enzymatic (SAVED) based on CRISPR-Cas12a targeting previously crucial variants, including Alpha, Beta, Gamma, Delta, Lambda, Mu, Kappa, currently circulating variant concern (VOC) Omicron its subvariants BA.1, BA.2, BA.3, BA.4, BA.5. SAVED...
Lipopolysaccharide is a major component of the cell wall Gram-negative bacteria and potent stimulator innate immune response via TLR4. Studies on LPS action both in vivo vitro have used different preparations LPS, including ultra-pure (LIST) less pure but expensive form (Sigma) isolated from Escherichia coli serotype O111:B4. The difference between effects these compounds has not been well studied although this information important understanding TLR stimulation. In study, we compared...
Abstract Epigenetic enzymes regulate higher-order chromatin architecture and cell-type specific gene expression. The ATPase BRG1 the SWI/SNF remodeling complex are epigenetic that accessibility during steady transitional cell states. Experiments in mice show loss of inhibits cellular reprogramming, while studies using human cells demonstrate overexpression enhances reprogramming. We hypothesized variation subunit expression population would contribute to variability efficiency induced...
Corona virus disease 2019 has spread worldwide, and appropriate drug design screening activities are required to overcome the associated pandemic. Using computational simulation, blockade mechanism of SARS-CoV-2 spike receptor binding domain (S RBD) human angiotensin converting enzyme 2 (hACE2) was clarified based on interactions between RBD hesperidin. Interactions anti-SARS-CoV-2 drugs therapy were investigated energy druggability compounds, they exhibited negative correlations; compounds...