A5068959088- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Diabetes and associated disorders
- COVID-19 Clinical Research Studies
- Evolution and Genetic Dynamics
- Cancer Research and Treatments
- Nanoplatforms for cancer theranostics
- RNA modifications and cancer
- Plant Gene Expression Analysis
- Plant Virus Research Studies
- COVID-19 diagnosis using AI
- Neurotransmitter Receptor Influence on Behavior
- SARS-CoV-2 detection and testing
- Plant tissue culture and regeneration
- Phytochemicals and Antioxidant Activities
- Neuroendocrine regulation and behavior
- Selenium in Biological Systems
- Photodynamic Therapy Research Studies
- Cancer-related molecular mechanisms research
- Metalloenzymes and iron-sulfur proteins
- Virus-based gene therapy research
- Circular RNAs in diseases
Hubei University
2019-2025
Sanya University
2025
Nanjing Agricultural University
2025
Henan Agricultural University
2024-2025
Nanjing University of Chinese Medicine
2021-2024
Center for Innovation
2019
<italic>Pf</italic>Ago-mediated Nucleic acid Detection (PAND) distinguishes single-nucleotide mutants and accomplishes multiplexed detection by a second round of cleavage.
Abstract Argonaute (Ago) proteins are conserved nucleic acid-guided present in all domains of life. Eukaryotic (eAgos) key players RNA interference pathways and function as RNA-guided endonucleases at physiological temperatures. Although eAgos considered to evolve from prokaryotic (pAgos), previously studied pAgos were unable catalyze cleavage Here, we describe a distinctive pAgo mesophilic bacteria Kurthia massiliensis (KmAgo). KmAgo utilizes DNA guides cleave single-stranded (ssDNA)...
Argonaute (Ago) proteins are programmable nucleases found in eukaryotes and prokaryotes. Prokaryotic Agos (pAgos) share a high degree of structural homology with eukaryotic (eAgos), eAgos originate from pAgos. Although exclusively cleave RNA targets, most characterized pAgos DNA targets. This study novel pAgo, MbpAgo, the psychrotolerant bacterium Mucilaginibacter paludis which prefers to targets rather than Compared previously studied Agos, MbpAgo can utilize both 5'phosphorylated(5'P)...
Abstract By leveraging the ability of bacteria to express therapeutic protein cytolysin A (ClyA) through plasmid transformation, a thermally‐activated biohybrid (TAB@Au) is constructed by biomineralizing gold nanoparticles (AuNPs) on E. coli surface. Due feature anaerobic homing tumor microenvironments, bacteria‐based antitumor vehicles can be efficaciously accumulated at sites. Under NIR laser irradiation, biomineralized AuNPs harvest transdermal photons and convert them into local heat for...
Abstract Given the special microenvironment of solid tumors, live microorganisms have emerged as drug delivery vehicles and therapeutic agents. Here, an acid‐induced platform is constructed using attenuated Escherichia coli to express cytolysin A protein. The bacteria can target colonize tumor tissues without causing notable host toxicity. Bacterial infection disrupt blood vessels trigger thrombosis in tissues, resulting cut‐off nutrient supply cells arrest growth. expression induced by...
Argonaute proteins are widespread in prokaryotes and eukaryotes with diversified catalytic activities. Here, we describe an from Marinitoga hydrogenitolerans (MhAgo) all eight cleavage Utilization of four types guides efficient single-stranded DNA (ssDNA) RNA targets revealed. The preference for the 5′-terminus nucleotides 5′P guides, but no obvious preferences that 5′OH is further uncovered. Moreover, efficiency heavily impaired by mismatches central 3′-supplementary regions affinity...
A simple and user-friendly nucleic acid sensing platform with 10 aM sensitivity, named USPCRP (combines ultrashort PCR Pyrococcus furiosus Argonaute cleavage for nuleic acids detection) is reported. The product of this could be directly used as a DNA guide to mediate PfAgo molecular beacons.
ABSTRACT Specific light wavelengths can affect plant responses to pathogens. In this study, we investigated the soft rot resistance of lettuce grown under different conditions and analyzed underlying molecular mechanisms using RNA‐seq data. The results revealed that blue‐light‐grown leaves had a better infection compared white other conditions, as evidenced by fewer symptoms lower incidence. After infection, H 2 O levels increased in all samples but were lowest leaves. Significantly higher...
Abstract PfAgo, a thermophilic Argonaute nuclease from Pyrococcus furiosus , is widely used in various fields due to its high DNA‐guided DNA cleavage activity. However, high‐temperature‐dependent activity largely restricts applications moderate‐temperature scenarios. In this study, PfAgo engineered for cold adaptation based on ternary complex structure and the attributes of cold‐adapted enzymes, yielding series variants with better performance at moderate temperatures. Among those, mPfAgo...
The kidney renal papillary cell carcinoma (KIRP) is a relatively rare type of (RCC). Currently, most cancer studies primarily focus on RCC, and there has been no investigation to find robust signature predict the survival outcome KIRP patients. In this study, we constructed competing endogenous RNA (ceRNA) network, including 1,251 lncRNA–miRNA–mRNA interactions. Eight differentially expressed genes (IGF2BP3, PLK1, LINC00200, NCAPG, CENPF, miR-217, GAS6-As1, LRRC4) based TCGA database were...
Abstract Pumpkin is widely used as a functional food for people and rootstock other Cucurbitaceae crops. Se-enriched pumpkin can be an important Se source humans. However, the boundary between beneficial phytotoxic levels of narrow. Here, effects selenite with different concentrations (2–80 μM) on growth accumulation in seedlings were explored. Our results revealed that accumulations both shoots roots C. moschata closely related to doses. Selenite seemed have no positive growth. The root...
China implemented a dynamic zero-COVID strategy to curb viral transmission in response the coronavirus disease 2019 (COVID-19) pandemic. This was designed inhibit mutation of severe acute respiratory syndrome 2 (SARS-CoV-2), virus responsible for COVID-19. study explores dynamics evolution under stringent non-pharmaceutical interventions (NPIs) through real-world observations. Our analysis encompassed all polymerase chain reaction (PCR)-confirmed COVID-19 cases (n = 780) from two consecutive...
ABSTRACT Argonaute proteins are widespread in prokaryotes and eukaryotes. Most prokaryotic (pAgos) use 5’P-gDNA to target complementary DNA. However, more studies on the properties of pAgos make their functions diversified. Previously reported only possess several forms high activity all eight cleavage patterns, which limits practical applications. Here, we described a unique pAgo from Marinitoga hydrogenitolerans (MhAgo) with activities. MhAgo can utilize four types guides (5’OH-gDNA,...
Abstract Within the local outbreak period of SARS-CoV-2 Delta variant in Nanjing and Yangzhou, China, we analyzed mutation process variants 520 cases, as well production, spread elimination new mutant strains under non-pharmaceutical interventions (NPI) strategy. The investigation on distribution COVID-19 cases phylogenetic analysis genome sequences attributed to tracking transmission chains, chains were terminated by isolation patients quarantine close-contracts, suggesting importance NPI...