A5038105587- Air Quality and Health Impacts
- Inhalation and Respiratory Drug Delivery
- Carcinogens and Genotoxicity Assessment
- Indoor Air Quality and Microbial Exposure
- Neonatal Respiratory Health Research
- Smoking Behavior and Cessation
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Genomics, phytochemicals, and oxidative stress
- Cystic Fibrosis Research Advances
- Aortic Disease and Treatment Approaches
- 3D Printing in Biomedical Research
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer Cells and Metastasis
- Asthma and respiratory diseases
- Dental Erosion and Treatment
- Cerebrospinal fluid and hydrocephalus
- Biomedical and Engineering Education
- Biological Stains and Phytochemicals
- Protease and Inhibitor Mechanisms
- Advanced Drug Delivery Systems
- Genetics, Bioinformatics, and Biomedical Research
- Effects and risks of endocrine disrupting chemicals
- Cardiac, Anesthesia and Surgical Outcomes
- Immune cells in cancer
- Radiopharmaceutical Chemistry and Applications
Japan Tobacco (Japan)
2016-2024
Japan Tobacco (United States)
2015
Osaka University
2009
RELX Group (Netherlands)
2004
Hy-Line (United States)
2004
Fukuoka Children's Hospital and Medical Center for Infectious Diseases
1995
University Medical Center
1994
University Hospital and Clinics
1994
Louisiana State University
1994
It has previously been found that, compared with cigarette smoke, the aerosols generated by heated tobacco products contain fewer and lower harmful potentially constituents (HPHCs) elicit biological activity in vitro models smoking-related exposure biomarker levels clinical studies. is important to accumulate such scientific evidences for a novel heating system, because different system may affect quantitative aspect of amount HPHCs qualitative aerosol generated. Here, chemical properties...
The use of novel tobacco- and nicotine-containing vapor products that do not combust tobacco leaves is on the rise worldwide. emissions these typically contain lower numbers levels potentially harmful chemicals compared with conventional cigarette smoke. These may therefore elicit fewer adverse biological effects. We effects from different types such products, i.e., our proprietary product (NTV), a commercially available heat-not-burn (HnB), e-cigarette (E-CIG), combustible in human...
Mucociliary clearance is the primary physical mechanism to protect human airways against harmful effects of inhaled particles. Environmental factors play a significant role in impairment this defense mechanism, whereas cigarette smoke discussed be one clinically most important causes. Impaired mucociliary smokers has been connected changes ciliated cells such as decreased numbers, altered structure and beat frequency. Clinical studies have shown that cilia length reduced healthy long-term...
The collagen gel contraction assay measures size to assess the of cells embedded in matrices. Using with lung fibroblasts is useful studying tissue remodeling process wound healing and disease development. However, involvement bronchial epithelial this should also be investigated. We applied a layer mucociliary differentiated onto matrices fibroblasts. This co-culture model enables direct contact between mesenchymal cells. stimulated culture transforming growth factor (TGF) β1 as an inducer...
Chemical respiratory sensitisation is a serious health problem. However, to date, there are no validated test methods available for identifying sensitisers. The aim of this study was develop an in vitro by modifying the human cell line activation (h-CLAT) detect sensitisers and distinguish them from skin THP-1 cells were exposed chemicals (two six sensitisers), either as monocultures or cocultures with air-liquid interface-cultured reconstructed bronchial epithelium. responses analysed...
Abstract Background The alveolar epithelium is exposed to numerous stimuli, such as chemicals, viruses, and bacteria that cause a variety of pulmonary diseases through inhalation. Alveolar epithelial cells (AECs) cultured in vitro are valuable tool for studying the impacts these stimuli developing therapies associated diseases. However, maintaining proliferative capacity AECs challenging. In this study, we used cocktail three small molecule inhibitors cultivate AECs: Y-27632, A-83-01,...
Epidermal growth factor (EGF) is present in biliary, pancreatic, and Brunner's gland secretions. The aim of the study was to assess effects EGF on lipid-induced mucosal injury. proximal jejunum anesthetized rats cannulated for perfusion lumen with emulsified oleic acid (40 mM 20 sodium taurocholate; pH 6.0). Mucosal epithelial integrity monitored by measuring blood-to-lumen clearance 51Cr-labeled EDTA. Perfusion lipid increased EDTA clearance. Addition (0.5 ng/ml) emulsion ameliorated...
Summary For the risk assessment of airborne chemicals, a variety in vitro direct exposure systems have been developed to replicate chemical vivo. Since cells at air-liquid interface are exposed cigarette smoke as an aerosol systems, it is possible reproduce situation human respiratory system using this device. However difficult know whether consistent with retained tract. The purpose study clarify point CULTEX ® RFS module which recently system. purpose, solanesol and acetaldehyde were...
Abstract Next‐generation tobacco products and nicotine delivery systems such as heat‐not‐burn electronic cigarettes, the usage of which is expected to have a beneficial impact on public health, gained popularity over past decade. However, risks associated with long‐term use are still incompletely understood. Although these should be clarified through epidemiological studies, studies normally performed based each product category, not product‐by‐product. Therefore, investigation risk...
Three-dimensional (3D) cultured primary cells are used to predict the toxicity of substances towards humans because these 3D cultures closely mimic physiological architecture tissues. Nonetheless, it is important consider primary-cell-specific variability for endpoint selection and appropriate evaluation donor-dependent characteristics may be retained even in vitro cell cultures. In this report, differentiated bronchial epithelial from three donors were investigate donor-to-donor...
Cigarette smoke is a known risk factor for inflammatory diseases in the respiratory tract, and exacerbation considered pivotal to pathogenesis of these diseases. Here, we performed two repeated exposure studies which exposed human bronchial epithelial tissues an organotypic culture model cigarette extract (CSE); first study was conducted over four-day period determine suitable dose range extended period, second one-month elucidate exposure-by-exposure effects tissues. We focused on matrix...
Abstract Cigarette smoke (CS) is a complex mixture of chemicals and interacts with various physiological processes. We previously reported that nuclear factor erythroid 2‐related 2 (NRF2) was the most sensitive transcription to aqueous CS extract (AqCSE) exposure in monolayer cultured human bronchial epithelial cell lines. Recently, vitro three‐dimensional (3D) culture models have been used supplement pharmacological toxicological assessments. Bronchial epithelium particular are useful for...
Abstract Objectives This in vitro study assessed the potential of tooth discoloration by aerosols generated from three heated tobacco products (HTPs) with different specifications: in‐direct heating system platform 1.0a (IT1.0a), 2.0a (IT2.0a), and direct 3.0a (DT3.0a). In addition, flavor types (regular, menthol, berry menthol) were selected for each HTP to characterize effect on discoloration. Material Methods Six bovine samples exposed directly one pack HTP: 350 puffs IT1.0a, 325 IT2.0a,...
Given the complexity of inhaled substances, aerosol exposure environment has seen diversification and development setups in conjunction with evolving vitro toxicology space. Each laboratory uses its system differently (different protocols, adaptations, biological analysis). Unfortunately, as systems diversify, so does comparing multiple a “standardized” manner. As yet, no one compared simply whether these diverse can all generate consistent stream, which is paramount prior to transit...
The use of reconstituted human airway (RHuA) epithelial tissues to assess functional endpoints is highly relevant in respiratory toxicology, but standardised methods are lacking. In June 2015, the Institute for Vitro Sciences (IIVS) held a technical workshop evaluate potential standardisation methods, including ciliary beat frequency (CBF). applicability protocol suggested was assessed multi-laboratory ring study. This report summarises findings, and uses similarities differences identified...
Summary A new reference cigarette, 1R6F, produced by the Kentucky Tobacco Research and Development Center, has been manufactured as a substitute for 3R4F cigarette because of depletion stock. The purpose current study was to investigate interchangeability 1R6F comparing chemical biological characteristics mainstream smoke assess inter-laboratory reproducibility results obtained in with previous report. We analyzed 45 priority chemicals required Health Canada regulatory reporting assessed...