A5041921949- Contact Dermatitis and Allergies
- Animal testing and alternatives
- Allergic Rhinitis and Sensitization
- Immunotoxicology and immune responses
- Dermatology and Skin Diseases
- Pesticide Exposure and Toxicity
- Skin Protection and Aging
- Advancements in Transdermal Drug Delivery
- Occupational exposure and asthma
- Immunotherapy and Immune Responses
- Effects and risks of endocrine disrupting chemicals
- Virus-based gene therapy research
- bioluminescence and chemiluminescence research
- Computational Drug Discovery Methods
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Response and Inflammation
- Photodynamic Therapy Research Studies
- Cytokine Signaling Pathways and Interactions
- Advanced biosensing and bioanalysis techniques
- Antibiotic Resistance in Bacteria
- Graphene and Nanomaterials Applications
- NF-κB Signaling Pathways
- Chemistry and Chemical Engineering
- Bacillus and Francisella bacterial research
- Biochemical effects in animals
National Institute of Health Sciences
2021-2024
Tohoku University
2023
National Institute of Health Sciences
2022
Shiseido Group (Japan)
2009-2018
Suzuki (Japan)
2014-2018
Japan Chemical Industry Association
2015
Pierre Fabre (France)
2014
Procter & Gamble (Germany)
2014
Cosmetics Europe
2014
Henkel (Germany)
2014
Sensitization, the prerequisite event in development of allergic contact dermatitis, is a key parameter both hazard and risk assessments. The pathways involved have recently been formally described OECD adverse outcome pathway (AOP) for skin sensitization. One single non-animal test method will not be sufficient to fully address this AOP many cases use battery tests necessary. A number methods are now developed validated. In order facilitate acceptance these by regulatory scientific...
The need for non-animal data to assess skin sensitisation properties of substances, especially cosmetics ingredients, has spawned the development many in vitro methods. As it is widely believed that no single method can provide a solution, Cosmetics Europe Skin Tolerance Task Force defined three-phase framework testing strategy sensitization potency prediction. results first phase – systematic evaluation 16 test methods are presented here. This involved generation on common set ten...
To develop a testing strategy incorporating the human cell line activation test (h-CLAT), direct peptide reactivity assay (DPRA) and DEREK, we created an expanded data set of 139 chemicals (102 sensitizers 37 non-sensitizers) by combining existing 101 through collaborative projects Japan Cosmetic Industry Association. Of additional 38 chemicals, 15 with relatively low water solubility (log Kow > 3.5) were selected to clarify limitation strategies regarding lipophilic chemicals....
We previously developed the human cell-line activation test (h-CLAT) in vitro skin sensitisation test, based on our reported finding that a 24-hour exposure of THP-1 cells (a monocytic leukaemia cell line) to sensitisers is sufficient induce augmented expression CD86 and CD54. The aim this study confirm predictive value h-CLAT for activity by employing larger number chemicals. One hundred chemicals were selected, according their categorisation local lymph node assay (LLNA), as being:...
Recent changes in regulatory restrictions and social opposition to animal toxicology experiments have driven the need for reliable vitro tests predicting skin sensitizing potentials of a wide variety industrial chemicals. Previously, we developed human cell line activation test (h-CLAT) as cell-based assay predict potential chemicals, showed correspondence between h-CLAT murine local lymph node results.This study was conducted investigate predictive performance potential.We selected total 66...
To meet the urgent need for a reliable alternative test predicting skin sensitizing potential of many chemicals, we have developed cell-based in vitro test, human Cell Line Activation Test (h-CLAT). However, predictive performance lipophilic chemicals h-CLAT still remains relatively unknown. Moreover, it's suggested that low water solubility might induce false negative outcomes. Thus, this study, tested soluble 37 with log Kow values above and below 3.5 h-CLAT. The small-scale assessment...
The skin sensitization potential of chemicals has been determined with the use murine local lymph node assay (LLNA). However, in recent years public concern about animal welfare led to a requirement for non-animal risk assessment systems prediction potential, replace LLNA. Selection an appropriate vitro test or silico model descriptors is critical obtain good predictive performance. Here, we investigated utility artificial neural network (ANN) models using various combinations from several...
It is important to predict the potential of cosmetic ingredients cause skin sensitization, and in accordance with European Union directive for replacement animal tests, several vitro tests based on adverse outcome pathway have been developed hazard identification, such as direct peptide reactivity assay, KeratinoSens™ human cell line activation test. Here, we describe development an artificial neural network (ANN) prediction model sensitization risk assessment integrated testing strategy...
Significant progress has been made in the development and validation of non-animal test methods for skin sensitization assessment. At present, three four key events Adverse Outcome Pathway (AOP) are assessable by OECD-accepted vitro methods. The fourth event describes immunological response draining lymph node where activated dendritic cells present major histocompatibility complex-bound chemically modified peptides to naive T cells, thereby priming proliferation antigen-specific cells....