A5039381595- Contact Dermatitis and Allergies
- Animal testing and alternatives
- Skin Protection and Aging
- Occupational exposure and asthma
- Immunotoxicology and immune responses
- Dermatology and Skin Diseases
- Effects and risks of endocrine disrupting chemicals
- Pharmacogenetics and Drug Metabolism
- Carcinogens and Genotoxicity Assessment
- Dyeing and Modifying Textile Fibers
- Drug-Induced Adverse Reactions
- Allergic Rhinitis and Sensitization
- Heavy Metal Exposure and Toxicity
- Mast cells and histamine
- Immunotherapy and Immune Responses
- Synthesis and Characterization of Heterocyclic Compounds
- Immune Response and Inflammation
- Inflammatory mediators and NSAID effects
- Antimicrobial agents and applications
- Nitric Oxide and Endothelin Effects
- Drug-Induced Hepatotoxicity and Protection
- Trace Elements in Health
- Hair Growth and Disorders
- bioluminescence and chemiluminescence research
- Computational Drug Discovery Methods
Deutsche Welle
2003-2024
European Space Operations Centre
2018
L'Oréal (France)
2018
San Gallicano Hospital
2018
Istituti di Ricovero e Cura a Carattere Scientifico
2018
Heidelberg University
2018
Henkel (Germany)
2014-2018
Sheffield Teaching Hospitals NHS Foundation Trust
2018
Kao Corporation (Germany)
2018
Technical University of Darmstadt
2017
Skin protein reactivity is a well established key step in the development of skin sensitization. Understanding relationship between chemical's ability to react with or modify and sensitization has led Direct Peptide Reactivity Assay (DPRA) our laboratory. A current limitation DPRA that it cannot readily measure pro-hapten chemical sensitizers. Pro-haptens are sensitizers not directly reactive must be bioactivated vivo form an electrophilic intermediate(s). Results from this work demonstrate...
Abstract: The 7th Amendment to the EU Cosmetics Directive prohibits use of animals in cosmetic testing for certain endpoints, such as genotoxicity. Therefore, skin vitro models have replace chemical vivo . However, metabolic competence neither human nor alternative has so far been fully characterized, although is first‐pass organ accidentally or purposely (cosmetics and pharmaceuticals) applied chemicals. Thus, there an urgent need understand xenobiotic‐metabolizing capacities compare these...
Several human skin models employing primary cells and immortalized cell lines used as monocultures or combined to produce reconstituted 3D constructs have been developed. Furthermore, these included in European genotoxicity sensitization/irritation assay validation projects. In order help interpret data, Cosmetics Europe (formerly COLIPA) facilitated research projects that measured a variety of defined phase I II enzyme activities created complete proteomic profile xenobiotic metabolizing...
Skin is important for the absorption and metabolism of exposed chemicals such as cosmetics or pharmaceuticals. The Seventh Amendment to EU Cosmetics Directive prohibits use animals cosmetic testing certain endpoints, genotoxicity; therefore, there an urgent need understand xenobiotic metabolizing capacities human skin compare these activities with reconstructed 3D models developed replace animal testing. We have measured Phase I enzyme cytochrome P450 (CYP) cyclooxygenase (COX) in ex vivo...
The need for alternative approaches to replace the in vivo rabbit Draize eye test evaluation of irritation cosmetic ingredients has been recognised by cosmetics industry many years. Extensive research lead development several assays, some which have undergone formal validation. Even though, date, no single vitro assay validated as a full replacement test, organotypic assays are accepted specific and limited regulatory purposes. Although not formally validated, other models used over decade...
For the assessment of genotoxic effects cosmetic ingredients, a number well-established and regulatory accepted in vitro assays are place. A caveat to use these is their relatively low specificity high rate false or misleading positive results. Due 7th amendment EU Cosmetics Directive ban on vivo genotoxicity testing for cosmetics that was enacted March 2009, it no longer possible conduct follow-up tests ingredients further assess relevance findings. COLIPA, European Association, has...
Understanding the mechanistic aspects involved in sensitization by chemicals will help to develop relevant preventive strategies. Many potential sensitizers are not directly immunogenic but require activation outside or inside skin nonenzymatic oxidation (prehaptens) metabolic transformation (prohaptens) prior being able induce an immune response. This necessary step has yet been actively integrated into a cell line–based prediction approach. We cocultured HaCaT keratinocytes with THP-1 as...
Characterisation of skin sensitisation potential is a key endpoint for the safety assessment cosmetic ingredients especially when significant dermal exposure to an ingredient expected. At present mouse local lymph node assay (LLNA) remains 'gold standard' test method this purpose however non-animal methods are under development that aim replace need new animal data. COLIPA (the European Cosmetics Association) funds extensive programme research, and evaluation helped coordinate early three...
Abstract Background The goal of the present study was to determine whether cytokines in peripheral blood naive NOD mice correlate with disease process and thereby would provide a marker for monitoring activity. Methods Female (5, 10 14–16 weeks age) were investigated cross‐sectional study. In group 14–16‐week‐old mice, non‐diabetic diabetic analysed as different subgroups. Th1 cytokine (IFN‐γ) Th2 (IL‐10) quantified serum by sandwich enzyme‐linked immunosorbent assay (ELISA). Pancreatic mRNA...
Significant progress has been made in the development and validation of non-animal test methods for skin sensitization assessment. At present, three four key events Adverse Outcome Pathway (AOP) are assessable by OECD-accepted vitro methods. The fourth event describes immunological response draining lymph node where activated dendritic cells present major histocompatibility complex-bound chemically modified peptides to naive T cells, thereby priming proliferation antigen-specific cells....
Despite a positive patch test reaction to para-phenylenediamine (PPD) and/or toluene-2,5-diamine (PTD), many people attempt continue dyeing their hair with products containing PPD or its derivatives.Investigation of elicitation reactions among PPD/PTD-allergic individuals dye the less sensitizing derivative 2-methoxymethyl (ME)-PPD.Elicitation were studied in 43 by 45-min pretest an ME-PPD-containing on forearm. Upon negative result this was followed exposure subsequent colour...