A5005611718- bioluminescence and chemiluminescence research
- Porphyrin Metabolism and Disorders
- Heme Oxygenase-1 and Carbon Monoxide
- Photoreceptor and optogenetics research
- Electron Spin Resonance Studies
- Insect and Pesticide Research
- Redox biology and oxidative stress
- Heavy Metal Exposure and Toxicity
- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Hemoglobin structure and function
- Biochemical effects in animals
- Neonatal Health and Biochemistry
- Vitamin C and Antioxidants Research
- Free Radicals and Antioxidants
- Photodynamic Therapy Research Studies
- Nitric Oxide and Endothelin Effects
- Advanced Glycation End Products research
- Mercury impact and mitigation studies
- Radical Photochemical Reactions
- Analytical Chemistry and Sensors
- Electrochemical sensors and biosensors
- Exercise and Physiological Responses
- Molecular Sensors and Ion Detection
- Insect Utilization and Effects
Universidade de São Paulo
2015-2024
Universidade Federal de São Paulo
2009-2021
Instituto Butantan
2017-2018
Instituto de Geofísica y Astronomía
2015
Instituto de Ciências Farmacêuticas
2011-2013
Instituto de Ciências Ambientais, Químicas e Farmacêuticas
2012
Universidade Federal do Paraná
2011
Hospital Universitário da Universidade de São Paulo
2010
Fundação de Apoio à Universidade Federal de São Paulo
2010
Universidade Cruzeiro do Sul
2005-2008
The dark side of melanin exposed Sun worshippers may have more to worry about than the DNA damage that occurs while they're relaxing on beach. It seems photoproducts responsible for cancer-causing mutations in skin cells continue be generated hours after sunlight exposure. Premi et al. find a key mediator this delayed is melanin, pigment thought protect against cancer (see Perspective by Taylor). They propose “chemiexcitation” model which reactive oxygen and nitrogen species induced...
Polymeric nanoparticles (PLGA) have been developed for the encapsulation and controlled release of quercetin catechin. Nanoparticles were fabricated using a solvent displacement method. Physicochemical properties measured by light scattering, scanning electron microscopy ζ ‐potential, X‐ray diffraction, infrared spectroscopy differential calorimetry. Encapsulation efficiency in vitro profiles obtained from pulse voltammetry experiments. Antioxidant free encapsulated flavonoids determined...
To spin trap hydroxyl radical (HO*) with in vivo detection of the resultant adducts, use two traps, 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) and 5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide (DEPMPO) (10 mmol/kg) has been compared. In mice treatment 5-aminolevulinic acid Fe3+ resulted adducts radicals (HO*), but only DEPMPO. Similarly, 'HO* adducts' generated via nucleophilic substitution SO4*- formed could be observed when using DEPMPO as trap. The reasons for differences between DMPO...
OF ANTIOXIDANTS.Growing knowledge on the health-promoting impact of antioxidants in everyday foods, combined with assumption that a number common synthetic preservatives may have hazardous side effects has led to increased investigations field natural antioxidants, principally those found plants.Food industries normally discard plant residues could benefit human health and diminish undesirable environmental impact.Once estimated content these residues, advantageous economical social...
Activities of superoxide dismutase (EC 1.15.1.1) and glutathione peroxidase 1.11.1.9) in erythrocytes were evaluated 50 schizophrenic 20 manic-depressive patients, who or not being treated with different neuroleptic drugs, results compared those for 58 normal individuals. Neuroleptic-treated untreated patients showed similar activities dismutase, about 60% higher than found individuals (p less 0.001). In either lithium (n = 8) plus drugs 12), increased by 40% over subjects Significantly...
Phrixothrix railroad-worms emit yellow-green light through 11 pairs of lateral lanterns along the body and red two cephalic lanterns. The cDNAs for luciferase vivianii, which green (λmax= 542 nm), head P. hirtus, most red-shifted bioluminescence 628 nm) among luminescent beetles, were cloned. Positive clones emitted (PvGR: λmax= 549 (PhRE: 622 isolated. lucifereases coded by PvGR (545 amino acid residues) PhRE (546 share 71% identity. luciferases showed 50−55% 46−49% identity with firefly...
Peroxynitrite and hydrogen peroxide are mediators of cytotoxicity. This study shows that the peroxynitrite anion reacts with to release oxygen accompanied by emission chemiluminescence (CL). Direct characterization this light attributes it transition singlet molecular triplet ground state. Chemiluminescence was monitored: (i) dimol in red spectral region (> 610 nm) using a red‐sensitive photomultiplier; (ii) monomol infrared (1270 liquid nitrogen‐cooled germanium diode. These properties...
Abstract This study examined the effect of experimental hyperand hypothyroidism on superoxide dismutase, catalase and glutathione peroxidase activities rat lymphoid organs (mesenteric lymph nodes, spleen thymus) muscles (soleus gastrocnemius-white portion) for comparison. The capacity generation reducing equivalents was also investigated: glucose-6-phosphate dehydrogenase (pentose-phosphate pathway) citrate synthase (Krebs cycle). Hyperthyroidism tended to enhance lipid peroxide content in...
Ethanol extracts of powdered genipap (Genipa americana L.), umbu (Spondia tuberosa A.) and siriguela purpurea L.) prepared from separate pulp, seeds peel were investigated for their (i) antioxidant capacity, which was evaluated by various known methods; (ii) acetylcholinesterase (AChE) inhibitory activity; (iii) cytotoxic effect on corneal epithelial cells sheep. The highest values total phenolic content obtained with seed extracts. Siriguela (seeds peel) displayed the activities. Lipid...
Abstract Four‐membered cyclic peroxides are high‐energy compounds often associated to cold light emission, but whose chemical and biological roles still matters of debate. The often‐dangerous synthesis 1,2‐dioxetanes, achieved around 50 years ago, has been mastered over the a point where some derivatives commercially available. This fact does not imply that 1,2‐dioxetanes can be conveniently prepared in gram scale or analogous 1,2‐dioxetanones elusive 1,2‐dioxetanedione simple. Important...
5-Aminolevulinic acid (ALA), a heme precursor accumulated in acute intermittent porphyria and lead poisoning, undergoes metal-catalyzed aerobic oxidation at physiological pH to yield reactive free radical species (O2., HO., ALA.). We analyzed the relationships between plasma ALA concentrations, blood concentrations of lead, protoporphyrin IX (PP-IX), superoxide dismutase (SOD), methemoglobin (metHb), urine chemiluminescence (CL) samples collected from lead-exposed workers. All variables...
Abstract. The larval click‐beetle Pyrearinus termitilluminans elicits the phenomenon of luminous termite mounds in central‐west region Brazil. bioluminescence (BL) spectrum this larva (Λ max = 534 run) is one most blue‐shifted reported among known luminescent Cole‐optera. We have isolated mRNA from thoracic lanterns and constructed a cDNA library into δZAP II vector. An expression was obtained after excision pBluescript plasmid. This screened by photodetection clone that emitted green BL (Δ...