A5086540684- Osteoarthritis Treatment and Mechanisms
- Bone Metabolism and Diseases
- Proteoglycans and glycosaminoglycans research
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- Veterinary Equine Medical Research
- Milk Quality and Mastitis in Dairy Cows
- Turtle Biology and Conservation
- Helminth infection and control
- TGF-β signaling in diseases
- Neurological diseases and metabolism
- T-cell and B-cell Immunology
- Connective tissue disorders research
- Blood Coagulation and Thrombosis Mechanisms
- Axon Guidance and Neuronal Signaling
- Animal Virus Infections Studies
- Parasitic Infections and Diagnostics
- Physiological and biochemical adaptations
- Inflammatory mediators and NSAID effects
- Protease and Inhibitor Mechanisms
- Silk-based biomaterials and applications
- Insect and Pesticide Research
- Parasitic infections in humans and animals
- Bird parasitology and diseases
The University of Melbourne
2009-2021
Roslin Institute
2011
University of Edinburgh
2011
Walter and Eliza Hall Institute of Medical Research
2003
Institute of Medical Microbiology and Hygiene
2003
Technical University of Munich
2003
Monash Institute of Medical Research
2003
Jagiellonian University
1983
University of Amsterdam
1983
The CD45RA(hi)CD11c(int) plasmacytoid predendritic cells (p-preDCs) of mouse lymphoid organs, when stimulated in culture with CpG or influenza virus, produce large amounts type I interferons and transform without division into CD8(+)CD205(-) DCs. P-preDCs express CIRE, the murine equivalent DC-specific intercellular adhesion molecule 3 grabbing nonintegrin (DC-SIGN). are divisible by CD4 expression two subgroups differing turnover rate response to Staphylococcus aureus. kinetics...
Cells that form bone (osteoblasts) express both ephrinB2 and EphB4, previous work has shown pharmacological inhibition of the eph-rinB2/EphB4 interaction impairs osteoblast differentiation in vitro vivo. The purpose this study was to determine role signaling lineage process formation. Cultured osteoblasts from mice with osteoblast-specific ablation showed delayed expression markers, a finding reproduced by ephrinB2, but not RNA interference. Microcomputed tomography, histomorphometry,...
Abstract Mineralized bone forms when collagen-containing osteoid accrues mineral crystals. This is initiated rapidly (primary mineralization), and continues slowly (secondary mineralization) until remodeled. The interconnected osteocyte network within the matrix differentiates from bone-forming osteoblasts; although osteoblast differentiation requires EphrinB2, osteocytes retain its expression. Here we report brittle bones in mice with osteocyte-targeted EphrinB2 deletion. not caused by low...
Articular cartilage is indispensable for joint function but has limited capacity self-repair. Engineering of neocartilage in vitro therefore a major target autologous repair arthritis. Previous analysis targeted cellular organization and specific molecular components. However, the complexity extracellular matrix (ECM) development not been investigated by proteomics. To redress this, we developed mouse culture system that produces cartilaginous ECM. Differential tissue proteome 3-week 3-day...
Metaphyseal chondrodysplasia, Schmid type (MCDS) is characterized by mild short stature and growth plate hypertrophic zone expansion, caused collagen X mutations. We recently demonstrated the central importance of ER stress in pathology MCDS recapitulating disease phenotype expressing misfolding forms (Schmid) or thyroglobulin (Cog) zone. Here we characterize Cog signaling networks transcriptional profiling microdissected mutant wildtype zones. Both models displayed similar unfolded protein...
In myotomes of the stickleback three main complexes muscle fibres were present. They comprised dwarf, intermediate and white fibres, which differed in regard to histochemical, immunohistochemical ultrastructural features. addition, dwarf divided into two categories on basis myofibrillar ATPase activity cross‐reactions with specific antisera. Among it was possible observe some ‘aberrant fibres’, characterized by low reactivity P‐myosin serum.
Herpesviruses have been reported in several marsupial species, but molecular classification has limited to four herpesviruses macropodids, a gammaherpesvirus two antechinus species (Antechinus flavipes and Antechinus agilis), potoroid, the eastern bettong (Bettongia gaimardi) gammaherpesviruses koalas (Phascolarctos cinereus). In this study we examined range of Australian marsupials for presence using serological techniques, also assessed risk factors associated with herpesvirus infection....
The majority of the skeleton arises by a process termed endochondral ossification, where cartilaginous templates expand and are resorbed osteoclasts then replaced osteoblastic bone formation. EphrinB2 is receptor tyrosine kinase expressed osteoblasts growth plate chondrocytes that promotes osteoblast differentiation inhibits osteoclast We investigated role ephrinB2 in ossification using osterix-Cre (Osx1Cre) targeted gene deletion. Neonatal Osx1Cre.EfnB2Δ/Δ mice exhibited transient...
Changes in the ovine mammary gland epithelium during initiated involution were studied by light and electron microscopy. Apoptosis of duct alveolar epithelial cells was first identified at 2 d after weaning, reached a peak 4 then progressed gradually thereafter. Apoptotic phagocytosed intraepithelial macrophages cells. Occasional apoptotic observed lumina. The highly vacuolated lumina confirmed to be as they CD45 + , MHC class II . myoepithelial involved shrinkage extension cytoplasmic...
Osteochondrosis is a condition involving defective endochondral ossification and retention of cartilage in subchondral bone. The pathophysiology this poorly characterized, but it has been proposed that the fundamental defect failure chondrocyte hypertrophy. aim current study was to characterize phenotypic changes chondrocytes associated with initiation osteochondrosis. Early lesions were induced an equine model osteochondrosis by feeding foals high energy diet for 8 or 15 weeks. Lesions...
ABSTRACT The tissue destruction seen in chronic periodontitis is commonly accepted to involve extensive upregulation of the host inflammatory response. Protease-activated receptor 2 (PAR-2)-null mice infected with Porphyromonas gingivalis did not display periodontal bone resorption contrast wild-type-infected and PAR-1-null-infected mice. Histological examination tissues confirmed lowered PAR-2-null identified a substantial decrease mast cells infiltrating these T from P. -infected or...
Abstract PAR-2 is expressed by osteoblasts and activated proteases present during inflammation. activation inhibited osteoclast differentiation induced hormones cytokines in mouse bone marrow cultures may protect from uncontrolled resorption. Introduction: Protease-activated receptor-2 (PAR-2), which osteoblasts, specifically a small number of proteases, including mast cell tryptase factor Xa. also peptide (RAP) that corresponds to the “tethered ligand” created cleavage receptor's...
Mammary glandular tissues and mammary secretions were obtained from sheep at 2–60 d after weaning to study the leucocyte phenotypes associated with involution. From 2–4 weaning, neutrophils predominant leucocytes in alveolar ductal lumina. Lymphocytes present epithelium, interalveolar periductal areas. Most of lymphocytes epithelium (IEL) CD8 + , some CD45R few CD4 . In clusters areas most There was a significant increase ( P < 0.05) percentages granulated IEL 2 7 this paralleled...
We detected herpesvirus infection in a male yellow-footed antechinus (Antechinus flavipes) and agile agilis) during the period of postmating immunosuppression mortality. Histopathologic examination tissues revealed lesions consistent with prostate both animals. Herpesvirus virions were observed by transmission electron microscopy tissue collected from antechinus. DNA was prostate, liver, lung, kidney, spleen, ocular/nasal using pan-herpesvirus PCR targeting viral polymerase. Nucleotide...
Surgical treatment of human hydatidsosis involves the use various scolicidal agents to kill infective Echinococcus granulosus protoscoleces that may disseminate into peritoneal cavity during surgery and potentially re-infect patient. Currently, no agent is completely effective in killing intracystic humans. Cyclosporin A (CsA) has previously been found be lethal for E. vitro. In this study, we further assessed effectiveness CsA as a by testing toxic effect at higher doses over time-periods....
During the early stages of articular osteochondrosis, cartilage is retained in subchondral bone, but pathophysiology this condition growing humans and domestic animals poorly understood. A subtractive hybridization study was undertaken to compare gene expression between experimentally induced equine osteochondrosis lesions control cartilage. Of many putative differentially expressed genes identified, eight were confirmed by quantitative PCR analysis as expressed, addition those already known...
Lactating animals are particularly susceptible to mastitis during the early stages of mammary gland involution following weaning. In this study we compared phagocytic capacity cells collected from sheep secretions at different involution. The ability neutrophils and macrophages ingest latex beads in an vitro phagocytosis assay was found be dependent on how heavily phagocytes were loaded with milk constituents. There a decline 1 2 days after weaning, while fully involuted glands more...
MalF has been shown to be required for virulence in the important avian pathogen Mycoplasma gallisepticum To characterize function of MalF, predicted part a putative ABC transporter, we compared metabolite profiles mutant with transposon inserted malF (MalF-deficient ST 04-1; ΔmalF) those wild-type bacteria using gas chromatography-mass spectrometry and liquid spectrometry. Of substrates likely transported by an transport system, glycerol was detected at significantly lower abundance ΔmalF...
Equine osteochondrosis results from a failure of endochondral ossification during skeletal growth. Endochondral involves chondrocyte proliferation, hypertrophy and death. Until recently no culture system was available to study these processes in equine chondrocytes.To optimise an vitro model which chondrocytes can be induced undergo physiological death as seen vivo.Chondrocytes isolated fetal or older (neonatal, growing mature) horses were cultured pellets 10% calf serum (FCS) horse (HS)....