A5037044585- Protease and Inhibitor Mechanisms
- Cell Adhesion Molecules Research
- Osteoarthritis Treatment and Mechanisms
- Blood Coagulation and Thrombosis Mechanisms
- Peptidase Inhibition and Analysis
- Bone and Dental Protein Studies
- Knee injuries and reconstruction techniques
- Angiogenesis and VEGF in Cancer
- Lipoproteins and Cardiovascular Health
- S100 Proteins and Annexins
- Inflammatory mediators and NSAID effects
- Rheumatoid Arthritis Research and Therapies
- Orthopaedic implants and arthroplasty
- Atherosclerosis and Cardiovascular Diseases
- Bone Metabolism and Diseases
- Vitamin K Research Studies
- dental development and anomalies
- Congenital Heart Disease Studies
- HER2/EGFR in Cancer Research
- Cardiovascular Conditions and Treatments
- Statistical Methods in Clinical Trials
- Macrophage Migration Inhibitory Factor
- Shoulder Injury and Treatment
- Elbow and Forearm Trauma Treatment
- Congenital Diaphragmatic Hernia Studies
Cardiff University
2024
Royal Children's Hospital
2007-2019
Murdoch Children's Research Institute
2005-2019
The University of Melbourne
1993-2019
University of East Anglia
1998-2000
The Royal Melbourne Hospital
1992-1997
Walter and Eliza Hall Institute of Medical Research
1992
University of Tasmania
1989
Gelatinase A and membrane-type metalloproteinase (MT1-MMP) were able to process human procollagenase-3 (Mr 60,000) the fully active enzyme (Tyr85 N terminus; Mr 48,000). MT1-MMP activated via a 56,000 intermediate (Ile36 terminus) 48,000 which was result of cleavage Glu84-Tyr85 peptide bond. We have established that activation rate by enhanced in presence progelatinase A, thereby demonstrating unique new cascade consisting three members matrix family.In addition, can be plasmin, cleaved...
We have assessed the effect of fibronectin and laminin-1 on expression molecules involved in activation pathway MMP-2, a key proteinase tissue remodelling. HT1080 fibrosarcoma cells cultured were shown to activate endogenous level comparable with that elicited by treatment phorbol ester. In contrast, MMP-2 expressed was mainly pro- (inactive form). Culture peptide fragments derived from central cell binding domain also promoted activation, indicating signals via integrin receptors may be...
SW1353 chondrosarcoma cells cultured in the presence of interleukin-1, concanavalin A or PMA secreted procollagenase 3 (matrix metalloproteinase-13). The enzyme was detected culture medium by Western blotting using a specific polyclonal antibody raised against recombinant human 3. Oncostatin M enhanced interleukin-1-induced production 3, whereas interleukin-4 decreased synthesis. latent except when had been treated with A, processed form 48 kDa, which corresponds to active form, found and...
The membrane‐type matrix metalloproteinases (MT‐MMPs) are a subclass of the metalloproteinase (MMP) family which uniquely possess C‐terminal transmembrane domain and initiators an activation cascade for progelatinase A (MMP‐2). Recent studies have shown that they can also efficiently directly degrade number macromolecules. We now show cells expressing MT1‐MMP on their cell surfaces cause subjacent proteolysis gelatin film this is inhibited by TIMP‐2 but not TIMP‐1. These data indicate...
Fragments of fibronectin occur naturally in vivo and are increased the synovial fluid arthritis patients. We have studied 45kDa fragment (Fn-f 45), representing N-terminal collagen-binding domain fibronectin, for its ability to modulate expression metalloproteinases by porcine articular chondrocytes vitro. report that stimulation cultured chondrocytes, or cartilage explants, with Fn-f 45 levels matrix metalloproteinase-13 (MMP-13; collagenase-3) released into conditioned medium a...
We have studied aggrecan catabolism mediated by matrix metalloproteinases (MMPs) in a porcine cartilage culture system. Using antibodies specific for DIPEN(341) and (342)FFGVG neoepitopes, we detected MMP-derived fragments conditioned medium cultured cartilage, radioimmunoassay, Western blotting, immunolocalization. Radioimmunoassay revealed that the amount (pmol of epitope/mg total glycosaminoglycan) epitope released from remained constant over 5-day period was not increased IL-1alpha or...
To characterize aggrecan catabolism and the overall phenotype in mice deficient both ADAMTS-4 ADAMTS-5 (TS-4/TS-5 Delta-cat) activity.Femoral head cartilage from joints of TS-4/TS-5 Delta-cat wild-type were cultured vitro, was stimulated with either interleukin-1alpha (IL-1alpha) or retinoic acid. Total release measured, aggrecanase activity examined by Western blotting using neoepitope antibodies for detecting cleavage at EGE 373-374 ALG, SELE 1279-1280 GRG, FREEE 1467-1468 GLG, AQE...
In the mouse, proteolysis in aggrecan interglobular domain is driven by ADAMTS-5, and mice deficient ADAMTS-5 catalytic activity are protected against loss cartilage damage experimental models of arthritis. Here we show that despite ablation activity, aggrecanolysis can still occur at two preferred sites chondroitin sulfate-rich region. Retinoic acid was more effective than interleukin-1α (IL) promoting cleavage these ADAMTS-5-deficient cartilage. These results suggest region mediated...
Abstract It is often assumed that macrophage-colony stimulating factor (M-CSF) or CSF-1, as well granulocyte macrophage-CSF (GM-CSF), can induce inflammatory mediator production by monocytes/macrophages. We demonstrate with elutriation-purified human monocytes that, in contrast to lipopolysaccharide, recombinant CSF-1 does not secretion of prostaglandin E2, interleukin-6 (IL-6), IL-lβ, tumor necrosis a, measured immunoassay; however, increased urokinase-type plasminogen activator (u-PA)...
Although exogenous corticosteroids advance structural maturation of the fetal lung, they can adversely affect lung and body growth. Our aim was to determine whether cortisol, at physiological doses, enhance hypoplastic without affecting Fetal sheep were divided into four groups ( n = 5 for each) hypoplasia (LH) induced in two groups. Increasing doses cortisol (1.5–4.0 mg) infused one group fetuses with LH LH; other received saline. retarded development, reduced tropoelastin mRNA levels,...