A5006426195- Hepatitis B Virus Studies
- Infectious Encephalopathies and Encephalitis
- Mosquito-borne diseases and control
- Respiratory viral infections research
- Cytomegalovirus and herpesvirus research
- Immune responses and vaccinations
- Viral Infections and Vectors
- Virus-based gene therapy research
- HIV Research and Treatment
- Virology and Viral Diseases
- HIV/AIDS Research and Interventions
- HIV/AIDS drug development and treatment
- Viral Infections and Outbreaks Research
- vaccines and immunoinformatics approaches
- Global Maternal and Child Health
- Insect symbiosis and bacterial influences
- Bacteriophages and microbial interactions
- Malaria Research and Control
- Palliative and Oncologic Care
- Public Health and Environmental Issues
- COVID-19 Impact on Reproduction
- HIV-related health complications and treatments
- HIV, Drug Use, Sexual Risk
- Immune Cell Function and Interaction
- HIV/AIDS oral health manifestations
National Autonomous University of Honduras
2011-2025
Karolinska Institutet
2009-2011
Swedish Institute
2009-2011
Taking CRISPR technology further techniques are allowing the development of technologies for nucleic acid detection (see Perspective by Chertow). advantages distinctive enzymatic properties enzymes, Gootenberg et al. developed an improved multiplexed quantitative and highly sensitive detection, combined with lateral flow visual readout. Myhrvold added a sample preparation protocol to create field-deployable viral diagnostic platform rapid specific strains pathogens in clinical samples....
Background: Palliative care (PC) can alleviate suffering and improve quality of life. Yet, disparities persist, particularly in Honduras. Training efforts PC for non-health caregivers have proven to reduce burnout stress while enhancing the life both patients. Purpose: This study aimed explore feasibility a training program Methods: exploratory utilized latent content analysis within an interpretivist paradigm. Data was collected through individual semi-structured interviews with 25...
Objective The Honduran HIV/AIDS Program began to scale up access HIV therapy in 2002. Up May 2008, more than 6000 patients received combination antiretroviral (cART). As drug resistance is the major obstacle for effective treatment, purpose of this study was assess prevalence HIV‐1‐infected individuals. Methods We collected samples from 138 individuals (97 adults and 41 children) on cART with virological, immunological or clinical signs treatment failure. HIV‐1 pol sequences were obtained...
Abstract Associations between HLA class I alleles and HIV progression in populations exhibiting Amerindian Caucasian genetic admixture remain understudied. Using univariable multivariable analyses we evaluated associations with five clinical parameters 3,213 clade B-infected, ART-naïve individuals from Mexico Central America (MEX/CAM cohort). A Canadian cohort (HOMER, n = 1622) was used for comparison. As expected, allele frequencies MEX/CAM HOMER differed markedly. In MEX/CAM, 13 - , 24 B...
<h2>Abstract</h2><h3>Background</h3> Genetic risk factors for dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS) and fever (DF) are limited, in particular there sparse data on genetic across diverse populations. <h3>Methods</h3> We conducted a genome-wide association study (GWAS) derivation validation sample of 7, 460 participants Latin American, South Asian, East Asian ancestries. then developed weighted polygenic score (PRS) each participant the cohorts three ancestries to predict...
The recent Zika virus (ZIKV) epidemic swept across Latin America and the Caribbean, where dengue (DENV) is endemic. antigenic similarities of these closely related flaviviruses left researchers clinicians with challenges to interpret serological tests. Thirty-six women attending a prenatal clinic in Honduras positive DENV IgM enzyme-linked immunoabsorbent assays (ELISAs) were screened ZIKV immunoglobulin M ELISA, reverse transcription polymerase chain reaction for 1-4, plaque reduction...
Introduction We assessed HIV drug resistance (DR) in individuals failing ART (acquired DR, ADR) and ART-naïve (pre-ART PDR) Honduras, after 10 years of widespread availability ART. Methods 365 HIV-infected, ART-naïve, 381 ART-experienced Honduran were enrolled 5 reference centres Tegucigalpa, San Pedro Sula, La Ceiba, Choluteca between April 2013 2015. Plasma protease-RT sequences obtained. HIVDR was using the WHO mutation list Stanford algorithm. Recently infected (RI) identified a...
Since its 2013 emergence in the Americas, Chikungunya virus (CHIKV) has posed a serious threat to public health. Early and accurate diagnosis of disease, though currently lacking clinics, is integral enable timely care epidemiological response. We developed dual detection system: CHIKV antigen E1/E2-based enzyme-linked immunosorbent assay (ELISA) lateral flow test using high-affinity anti-CHIKV antibodies. The ELISA was validated with 100 PCR-tested acute fever samples from Honduras. had an...
The humoral immune response in Honduran dengue hemorrhagic fever (DHF) hospitalized pediatric cases from the epidemics of 2004 and 2005 was studied sera collected 5 to 7 days onset. A total 145 were included study: 40 classified as primary with DHF Grade I or II 86 secondary; them, 73 13 shock syndrome (DSS) III IV. highest number found children < 1 year age. secondary observed between 10 years IgA values showed a statistically significant difference groups. relationship antibody responses...
<ns4:p><ns4:bold>Background:</ns4:bold> Global outbreaks caused by emerging or re-emerging arthropod-borne viruses (arboviruses) are becoming increasingly more common. These pathogens include the mosquito-borne belonging to <ns4:italic>Flavivirus</ns4:italic> and <ns4:italic>Alphavirus </ns4:italic>genera. often cause non-specific asymptomatic infection, which can confound viral prevalence studies. In addition, many acute phase diagnostic tests rely on detection of components such as RNA...
Antiretroviral therapy has had a great impact on the prevention of mother-to-child transmission (MTCT) HIV-1. However, development drug resistance, which could be subsequently transmitted to child, is major concern. In Honduras and Belize prevalence resistance among HIV-1-infected children remains unknown. A total 95 dried blood spot samples was obtained from HIV-1-infected, untreated in born during 2001 2004, when preventive antiretroviral often suboptimal consisted monotherapy with...
<ns4:p><ns4:bold>Background:</ns4:bold> Global outbreaks caused by emerging or re-emerging arthropod-borne viruses (arboviruses) are becoming increasingly more common. These pathogens include the mosquito-borne belonging to <ns4:italic>Flavivirus</ns4:italic> and <ns4:italic>Alphavirus </ns4:italic>genera. often cause non-specific asymptomatic infection, which can confound viral prevalence studies. In addition, many acute phase diagnostic tests rely on detection of components such as RNA...
Since its 2013 emergence in the Americas, chikungunya virus (CHIKV) has posed a serious threat to public health. Early and accurate diagnosis of disease, though currently lacking clinics, is integral enable timely care epidemiological response. We developed dual detection system: CHIKV antigen E1/E2-based enzyme-linked immunosorbent assay (ELISA) lateral flow test using high-affinity anti-CHIKV antibodies. The ELISA was validated with 100 PCR-tested acute Chikungunya fever samples from...
The impact of Zika virus (ZIKV) infection on pregnancies shows regional variation emphasizing the importance studies in different geographical areas. We conducted a prospective study Tegucigalpa, Honduras, recruiting 668 pregnant women between July 20, 2016, and December 31, 2016. performed Trioplex real-time reverse transcriptase-PCR (rRT-PCR) 357 serum samples taken at first prenatal visit. presence ZIKV was confirmed seven (7/357, 2.0%). Nine babies (1.6%) had microcephaly (head...
Zika virus (ZIKV) is a mosquito-borne
Abstract Metagenomic sequencing has the potential to transform microbial detection and characterization, but new tools are needed improve its sensitivity. We developed CATCH (Compact Aggregation of Targets for Comprehensive Hybridization), a computational method enhance nucleic acid capture enrichment diverse taxa. designs compact probe sets that achieve full coverage known sequence diversity scale well with this diversity. To illustrate applications CATCH, we focused on capturing viral...
<ns4:p><ns4:bold>Background:</ns4:bold> Global outbreaks caused by emerging or re-emerging arthropod-borne viruses (arboviruses) are becoming increasingly more common. These pathogens include the mosquito-borne belonging to <ns4:italic>Flavivirus</ns4:italic> and <ns4:italic>Alphavirus </ns4:italic>genera. often cause non-specific asymptomatic infection, which can confound viral prevalence studies. In addition, many acute phase diagnostic tests rely on detection of components such as RNA...
Purpose: Reflective practitioners embody the ability to critique their own clinical thinking about dilemmas that frequently arise in professional practice and everyday life. Conflicting evidence exists on whether or not self-reflective practices are effective promoting academic, clinical, personal success. This quantitative study investigated self-reflection as a predictor of increased occupational competence performance Level II Fieldwork for entry-level Master's degree therapy students....
Materiales y Metodos. La poblacion de estudio consistio 134 pacientes clinicamente clasificados con dengue hemorragico segun los criterios la OMS; 61 ≤5 dias enfermedad 73 ≥6 enfermedad. En enfermedad, 48 tenian diagnostico laboratorio positivo para 13 negativo por RT-PCR, estas muestras fueron analizadas prueba rapida AgNS1-Bioline ELISA Ag-NS1; ambas pruebas fabricadas Standard Diagnostics (SD) Corea del Sur. Las que ya (n=57) (n=16) en captura casera MAC-ELISA, Acs-IgM Bioline-SD...
El objetivo de la investigación fue determinar prevalencia resistencia del VIH a las drogas antirretrovirales en pacientes tratados y no tratados. Para tal efecto, se analizaron 100 muestras positivos, 50 previos iniciar tratamiento bajo con evidencia falla terapéutica, Tegucigalpa.Secuencias gen pol VIH-1 fueron generadas para presencia mutaciones asociadas resistencia, utilizando herramienta calibrada vigilancia propuesta por OMS pretratados lista base datos Stanford tratamiento.Los...