Hydrogel‐based, molecular permeable electronic devices are considered to be promising for electrical stimulation and recording of living tissues, either in vivo or vitro. This study reports the fabrication first hydrogel‐based that remain highly electrically conductive under substantial stretch bending. Using a simple technique involving combination chemical polymerization electropolymerization poly (3,4‐ethylenedioxythiophene) (PEDOT), tight bonding composite PEDOT polyurethane (PU) an...

We report herein the micropatterning of poly(3,4-ethylenedioxythiophene) (PEDOT) on a hydrogel, agarose, to provide fully organic, moist, and flexible electrode. The PEDOT/agarose electrodes were prepared through two electrochemical processes: electropolymerization PEDOT into hydrogel electrochemical-actuation-assisted peeling. also present typical application electrode cultivation contractile myotubes.

This study is the first report demonstrating proof-of-concept for a hydrogel-based touch sensor pad used non-invasive extraction and detection of sweat components. The device was composed an electrochemical L-lactate biosensor covered with agarose gel in phosphate buffer saline. When human skin contacts gel, continuously extracted into followed by in-situ potentiometric without controlled conditions. novel type expected to enable simple, daily periodic monitoring biomarkers advanced personal...

A completely organic iontophoresis patch is reported. built-in biofuel cell mounted on the that generates transdermal iontophoretic administration of compounds into skin. The amplitude current tuned by integrating a conducting polymer-based stretchable resistor predetermined resistance. As service to our authors and readers, this journal provides supporting information supplied authors. Such materials are peer reviewed may be re-organized for online delivery, but not copy-edited or typeset....

We report a porous polymer microneedle array with continuous micropores that has high mechanical strength and water absorption speed.

A stretchable, electrochromic film of a uniform composite poly(3,4-ethylenedioxythiophene):p-toluene sulfonic acid (PEDOT:PTS) and polyurethane (PU) (PEDOT/PU) was fabricated, its integration with hydrogel as free-standing, stretchable (EC) display demonstrated. The PEDOT/PU prepared by the spin coating solution containing an EDOT monomer PU, followed oxidative polymerization using iron(III) tosylate at elevated temperature. fabricated showed reversible electrochromism without external...

Abstract Angiogenesis plays a critical role in many diseases, including macular degeneration. At present, the pathological mechanisms remain unclear while appropriate models dissecting regulation of angiogenic processes are lacking. We propose an vitro angiogenesis process and test it by examining co-culture human retinal pigmental epithelial cells (ARPE-19) umbilical vein endothelial (HUVEC) inside microfluidic device. From characterisation APRE-19 monoculture, tight junction protein (ZO-1)...

Scanning ion conductance microscopy (SICM) using a nanopipette as probe and ionic current feedback signal was introduced novel technique to study live cells in physiological environment. To avoid contact between the pipette tip during conventional lateral scanning mode, we adopted standing approach (STA) mode which moved vertically first then retracted from cell surface at each measurement point on an XY plane. The STA ensured non-contact imaging of topography for wide range uneven...

Abstract Electrochemical sensor systems with integrated amplifier circuits play an important role in measuring physiological signals via situ human perspiration analysis. Signal processing circuitry based on organic thin-film transistors (OTFTs) have significant potential realizing wearable devices due to their superior mechanical flexibility and biocompatibility. Here, we demonstrate a novel potentiometric electrochemical sensing system comprised of potassium ion (K + ) employing OTFT-based...

We have developed gel sheet-supported C(2)C(12) myotube micropatterns and combined them with a microelectrode array chip to afford skeletal muscle cell-based bioassay system. Myotube line patterns cultured on glass substrate were transferred 100% efficiency the surface of fibrin sheets. The contractile behavior each pattern was individually controlled by localized electrical stimulation using arrays that had been previously modified electropolymerized poly(3,4-ethylenedioxythiophene)...

Abstract Wearable sensor device technologies, which enable continuous monitoring of biological information from the human body, are promising in fields sports, healthcare, and medical applications. Further thinness, light weight, flexibility low-cost significant requirements for making devices attachable onto tissues or clothes like a patch. Here we demonstrate flexible printed circuit system consisting an enzyme-based amperometric sensor, feedback control amplification circuits based on...

Contractile C(2)C(12) myotube line patterns embedded in a fibrin gel have been developed to afford physiologically relevant and stable bioassay system. The myotube/fibrin system was prepared by transferring monolayer from glass substrate while retaining the original of myotubes. To endow myotubes with contractile activity, series electrical pulses applied through pair carbon electrodes placed at either side separately. frequency magnitude contraction were functions pulse duration,...

Abstract A printed extended‐gate‐type organic transistor‐based enzyme sensor for the continuous monitoring of D‐glucose has been developed through a simple modification electrical circuit. The is composed glucose oxidase (GOx)/Prussian blue (PB)‐modified extended‐gate electrode and an Ag/AgCl reference electrode, which are connected to gate source electrodes transistor, respectively. GOx/PB‐modified were short‐circuited by external resistor spontaneously induce redox cycling PB. Continuous...

This review describes recent advances in biosensors for non-invasive human healthcare applications, especially focusing on sweat analysis, along with approaches fabricating these based printed electronics technology. Human contains various kinds of biomarkers. The relationship between a trace amount biomarkers partially partitioned from blood and diseases has been investigated by omic analysis. Recent progress wearable or portable enabled periodic continuous monitoring some while supporting...

A novel microfluidic device with an array of analytical chambers was developed in order to perform single-cell-based gene-function analysis. series processes carried out using the device, including electrophoretic manipulation single cells and electrochemical measurement gene function. poly(dimethylsiloxane) microstructure a channel (150 microm width, 10 height) chamber (100 x 20 (3)) were fabricated aligned on glass substrate Au microelectrodes. Two microelectrodes positioned employed as...

Electrochemical monitoring of cellular signal transduction under three-dimensional (3-D) cell culture conditions has been demonstrated by combining cell-based microarrays with a secreted alkaline phosphatase (SEAP) reporter system. The cells were genetically engineered to produce SEAP the control nuclear factor κB (NFκB) enhancer elements, and they embedded small volume collagen gel matrix on pyramidal-shaped silicon microstructure. Cellular expression triggered NFκB activation was assessed...

We report the fabrication of totally organic hydrogel-based microelectrodes poly(3,4-ethylenedioxythiophene) (PEDOT), which exhibit a lowered sheet resistivity about 100 Ω/□. The preparation process starts with electrodeposition conductive PEDOT (ca. 20 S cm-1) on Pt microelectrodes. After laminating hydrogels onto PEDOT-modified electrode substrates, second (low conductivity) layer was electrodeposited to anchor first film hydrogel. Finally, hydrogel micropatterns peeled off by taking...

Abstract A hydrogel‐based microchamber with organic electrodes for efficient electrical stimulations of human induced pluripotent stem cell‐derived cardiomyocytes (hiPSC‐CMs) is described. The made from molecularly permeable, optically transparent, and electrically conductive polyvinyl alcohol (PVA) hydrogel highly capacitive carbon electrode modified poly(3,4‐ethylenedioxythiophene) (PEDOT). Spheroids hiPSC‐CMs are cultured in microchambers, stimulated by the maturation. large interfacial...

Portable or wearable biosensors that can monitor physiological conditions from compounds non-invasively collected bodily fluids have been actively developed to realize predictive preventive personalized medicine. In particular, sweat is attracting much attention as a potential fluid source because it easily accessible the human skin surface and contains certain biomarkers partitioned blood. However, concentration of lower than in blood, highly sensitive biosensor required quantify them....