A5076040532- 3D Printing in Biomedical Research
- Neuroscience and Neural Engineering
- Microfluidic and Bio-sensing Technologies
- Retinal Development and Disorders
- Advanced Sensor and Energy Harvesting Materials
- Retinal Diseases and Treatments
- Microfluidic and Capillary Electrophoresis Applications
- Electrospun Nanofibers in Biomedical Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Retinal and Macular Surgery
- Tissue Engineering and Regenerative Medicine
- Nanofabrication and Lithography Techniques
- Cellular Mechanics and Interactions
- Electrochemical sensors and biosensors
- Muscle activation and electromyography studies
- Conducting polymers and applications
- Glaucoma and retinal disorders
- Photoreceptor and optogenetics research
- Advanced Drug Delivery Systems
- Corneal Surgery and Treatments
- Pregnancy and preeclampsia studies
- Additive Manufacturing and 3D Printing Technologies
- Electrochemical Analysis and Applications
- Bone Tissue Engineering Materials
- Analytical Chemistry and Sensors
Tokyo Medical and Dental University
2022-2024
Institute of Science Tokyo
2024
The University of Tokyo
2022-2023
Tohoku University
2012-2021
Graduate School USA
2012-2021
Sendai University
2015-2017
Brigham and Women's Hospital
2010-2012
Harvard University
2010-2012
Massachusetts Institute of Technology
2010-2011
Harvard–MIT Division of Health Sciences and Technology
2010-2011
Dielectrophoresis is used to align carbon nanotubes (CNTs) within gelatin methacrylate (GelMA) hydrogels in a facile and rapid manner. Aligned GelMA-CNT show higher electrical properties compared with pristine randomly distributed CNTs GelMA hydrogels. The muscle cells cultured on these materials demonstrate maturation As service our authors readers, this journal provides supporting information supplied by the authors. Such are peer reviewed may be re-organized for online delivery, but not...
To engineer tissue-like structures, cells must organize themselves into three-dimensional (3D) networks that mimic the native tissue microarchitecture. Microfabricated hydrogel substrates provide a potentially useful platform for directing biomimetic architecture in vitro. Here, we present microgrooved methacrylated gelatin hydrogels as suitable to build muscle-like fibrous structures facile and highly reproducible fashion. Microgrooved with two different ridge sizes (50 100 μm) were...
The initiation of human pregnancy is marked by the implantation an embryo into uterine environment; however, underlying mechanisms remain largely elusive. To address this knowledge gap, we developed hormone-responsive endometrial organoids (EMO), termed apical-out (AO)–EMO, which emulate in vivo architecture tissue. AO-EMO comprise exposed apical epithelium surface, dense stromal cells, and a self-formed endothelial network. When cocultured with embryonic stem cell–derived blastoids,...
Abstract Human placental villi have essential roles in producing hormones, mediating nutrient and waste exchange, protecting the fetus from exposure to xenobiotics. trophoblast organoids that recapitulate structure of could provide an important vitro tool understand development transplacental passage However, such do not currently exist. Here we describe generation using human stem (TS) cells. Following treatment with three kinds culture medium, TS cells form spherical a single outer layer...
We present a soft lithographic method to fabricate multiphenotype cell arrays by capturing cells within an array of reversibly sealed microfluidic channels. The technique uses reversible sealing elastomeric polydimethylsiloxane (PDMS) molds on surfaces sequentially deliver various fluids or onto specific locations substrate. Microwells the substrate were used capture and immobilize low shear stress regions inside By using channels it was possible deposit multiple types, such as hepatocytes,...
Establishing the 3D microscale organization of cells has numerous practical applications, such as in determining cell fate (e.g., proliferation, migration, differentiation, and apoptosis) making functional tissue constructs. One approach to spatially pattern is by dielectrophoresis (DEP). DEP characteristics that are important for manipulation, high accuracy, speed, scalability, ability handle both adherent non-adherent cells. However, widespread application this method largely restricted...
Hydrogel‐based, molecular permeable electronic devices are considered to be promising for electrical stimulation and recording of living tissues, either in vivo or vitro. This study reports the fabrication first hydrogel‐based that remain highly electrically conductive under substantial stretch bending. Using a simple technique involving combination chemical polymerization electropolymerization poly (3,4‐ethylenedioxythiophene) (PEDOT), tight bonding composite PEDOT polyurethane (PU) an...
A microengineered hybrid hydrogel array is developed containing asymmetrical extracellular matrices [polyethylene-glycol (PEG) and gelatin methacrylate (GelMA)] to mimic the anisotropic stem cell niche induce patterned differentiation of embryoid bodies (EBs). The around individual aggregate mouse EBs polarity demonstrate potential microscale engineered materials for forming tissues.
Abstract Angiogenesis plays a critical role in many diseases, including macular degeneration. At present, the pathological mechanisms remain unclear while appropriate models dissecting regulation of angiogenic processes are lacking. We propose an vitro angiogenesis process and test it by examining co-culture human retinal pigmental epithelial cells (ARPE-19) umbilical vein endothelial (HUVEC) inside microfluidic device. From characterisation APRE-19 monoculture, tight junction protein (ZO-1)...
Abstract The first cell fate commitment during mammalian development is the specification of inner mass and trophectoderm. This irreversible should be epigenetically regulated, but precise mechanism largely unknown in humans. Here, we show that naïve human embryonic stem (hES) cells can transdifferentiate into trophoblast (hTS) cells, primed hES cannot. Our transcriptome methylome analyses reveal a primate-specific miRNA cluster on chromosome 19 (C19MC) active silenced ones. Moreover, genome...
Engineered skeletal muscle tissues could be useful for applications in tissue engineering, drug screening, and bio-robotics. It is well-known that cells are able to differentiate under electrical stimulation (ES), with an increase myosin production, along the formation of myofibers contractile proteins. In this study, we describe use interdigitated array electrodes as a novel platform electrically stimulate engineered tissues. The resulting were analyzed quantified terms their myotube...
Controlling the cellular microenvironment can be used to direct organization, thereby improving function of synthetic tissues in biosensing, biorobotics, and regenerative medicine. In this study, we were inspired by microstructure biological properties extracellular matrix develop freestanding ultrathin polymeric films (referred as "nanomembranes") that flexible, cell adhesive, had a morphologically tailorable surface. The resulting nanomembranes exploited flexible substrates on which...
Engineered muscle tissues demonstrate properties far from native tissue. Therefore, fabrication of with enhanced functionalities is required to enable their use in various applications. To improve the formation mature higher functionalities, we co-cultured C2C12 myoblasts and PC12 neural cells. While alignment was obtained by culturing cells micropatterned methacrylated gelatin (GelMA) hydrogels, studied effects (PC12) on maturation tissues. Myoblasts cultured presence showed improved...
Organ-on-chip (OoC) platforms are a new class of advanced microfluidic devices that have been attracting significant attention for drug development since they can allow faster and more patient specific evaluation the effects drugs due to mimicking organ human models. As most devices, great majority OoC is made from polydimethylsiloxane (PDMS), easiness handle its physicochemical properties. Nevertheless, PDMS leach uncrosslinked oligomer absorb small hydrophobic molecules, which makes it...