A5101503187- Cellular transport and secretion
- Acute Myeloid Leukemia Research
- Pancreatic function and diabetes
- Hemoglobinopathies and Related Disorders
- Semiconductor Quantum Structures and Devices
- Dermatology and Skin Diseases
- Epigenetics and DNA Methylation
- Genomics, phytochemicals, and oxidative stress
- Calcium signaling and nucleotide metabolism
- Antioxidant Activity and Oxidative Stress
- Asthma and respiratory diseases
- Renal and related cancers
- Prenatal Screening and Diagnostics
- RNA modifications and cancer
- Advancements in Semiconductor Devices and Circuit Design
- Edible Oils Quality and Analysis
- Quantum and electron transport phenomena
- Pregnancy and preeclampsia studies
- Phytochemicals and Antioxidant Activities
- Tea Polyphenols and Effects
- Effects and risks of endocrine disrupting chemicals
- Signaling Pathways in Disease
- IL-33, ST2, and ILC Pathways
- Free Radicals and Antioxidants
- Vanadium and Halogenation Chemistry
Osaka University
1989-2025
Tohoku University
2013-2024
KOSÉ (Japan)
2021
Gunma University
2011-2020
Tokyo Institute of Technology
2017
Graduate School USA
2016
Sendai University
2016
Ochanomizu University
2013
Toho University
2006-2007
Tohoku Institute of Technology
2003
Nrf2 (NF-E2-related factor-2) transcription factor regulates oxidative/xenobiotic stress response and also represses inflammation. However, the mechanisms how alleviates inflammation are still unclear. Here, we demonstrate that interferes with lipopolysaccharide-induced transcriptional upregulation of proinflammatory cytokines, including IL-6 IL-1β. Chromatin immunoprecipitation (ChIP)-seq ChIP-qPCR analyses revealed binds to proximity these genes in macrophages inhibits RNA Pol II...
The initiation of human pregnancy is marked by the implantation an embryo into uterine environment; however, underlying mechanisms remain largely elusive. To address this knowledge gap, we developed hormone-responsive endometrial organoids (EMO), termed apical-out (AO)–EMO, which emulate in vivo architecture tissue. AO-EMO comprise exposed apical epithelium surface, dense stromal cells, and a self-formed endothelial network. When cocultured with embryonic stem cell–derived blastoids,...
Abstract Human placental villi have essential roles in producing hormones, mediating nutrient and waste exchange, protecting the fetus from exposure to xenobiotics. trophoblast organoids that recapitulate structure of could provide an important vitro tool understand development transplacental passage However, such do not currently exist. Here we describe generation using human stem (TS) cells. Following treatment with three kinds culture medium, TS cells form spherical a single outer layer...
Abstract The first cell fate commitment during mammalian development is the specification of inner mass and trophectoderm. This irreversible should be epigenetically regulated, but precise mechanism largely unknown in humans. Here, we show that naïve human embryonic stem (hES) cells can transdifferentiate into trophoblast (hTS) cells, primed hES cannot. Our transcriptome methylome analyses reveal a primate-specific miRNA cluster on chromosome 19 (C19MC) active silenced ones. Moreover, genome...
Abstract NF-E2-related factor-2 (Nrf2) regulates cellular responses to oxidative and electrophilic stress. Loss of Keap1 increases Nrf2 protein levels, Keap1-null mice die oesophageal hyperkeratosis because hyperactivation. Here we show that deletion in allows survival until adulthood, but the animals develop polyuria with low osmolality bilateral hydronephrosis. This phenotype is caused by defects water reabsorption are result reduced aquaporin 2 levels kidney. Renal tubular promotes...
Eukaryotic cells maintain protein homeostasis through the activity of multiple basal and inducible systems, which function in concert to allow adapt a wide range environmental conditions. Although transcriptional programs regulating individual pathways have been studied detail, it is not known how different are transcriptionally integrated such that deficiency one pathway can be compensated by change an auxiliary response. One plays essential role many proteostasis responses...
The placenta serves as the interface between mother and fetus, facilitating exchange of gases nutrients their separate blood circulation systems. Trophoblasts in play a central role this process. Our current understanding mammalian trophoblast development relies largely on mouse models. However, given diversification placentas, findings from cannot be readily extrapolated to other species, including humans. To fill knowledge gap, we performed CRISPR knockout screening human stem cells...
In a search for possible antitumor agents from natural sources, megastigmane glycosides and polyphenolic constituents isolated the leaves of Eriobotrya japonica (Rosaceae) were found to inhibit 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced activation Epstein−Barr virus early antigen in Raji cells. Roseoside procyanidin B-2 among active compounds an vitro assay; these further assessed activity vivo two-stage carcinogenesis assay on mouse skin. significantly delayed induced by...
Abstract Macrophages manifest distinct phenotype according to the organs in which they reside. In addition, flexibly switch their character adaptation changing environment. However, molecular basis that explains conversion of macrophage has so far been unexplored. We find CD169+ macrophages change by regulating level a transcription factor Maf both vitro and vivo C57BL/6J mice. When were exposed bacterial components, expressed an array acute inflammatory response genes Maf-dependent manner...
The elucidation of lipid oxidation mechanisms food is vital. In certain lipids, characteristic hydroperoxide isomers are formed by different (i.e., photo-oxidation or auto-oxidation). For example, linoleic acid photo-oxidized to 13-9Z, 11E-hydroperoxyoctadecadienoic (HPODE), 12-9Z,13E-HPODE, 10-8E,12Z-HPODE and 9-10E,12Z-HPODE, whereas 11E-HPODE, 13-9E,11E-HPODE, 9-10E,12Z-HPODE 9-10E,12E-HPODE auto-oxidation. Therefore, we considered that could be evaluated analyzing these positional...
Granuphilin, an effector of the small GTPase Rab27a, mediates stable attachment (docking) insulin granules to plasma membrane and inhibits subsequent fusion docked granules, possibly through interaction with a fusion-inhibitory Munc18-1/syntaxin complex. However, phenotypes exocytosis differ considerably between Rab27a- granuphilin-deficient pancreatic β cells, suggesting that other Rab27a effectors function in those cells. We found one putative family proteins, exophilin7/JFC1/Slp1, is...
Transcription factor GATA1 regulates the expression of a cluster genes important for hematopoietic cell differentiation toward erythroid and megakaryocytic lineages. Three functional domains have been identified in GATA1, transactivation domain located N terminus (N-TAD) two zinc finger middle molecule. Although N-TAD is known as solitary clinical observations Down syndrome leukemia suggest that there may be additional domains. In this study, we found reporter co-transfection assays activity...
“Sensitized thermal cells” do not rely on the Seebeck effect and require a temperature difference.
Direct observation of fluorescence-labeled secretory granule exocytosis in living pancreatic β-cells has revealed heterogeneous prefusion behaviors: some granules dwell beneath the plasma membrane before fusion, while others fuse immediately once they are recruited to membrane. Although former mode seems follow sequential docking-priming-fusion steps as found synaptic vesicle exocytosis, latter mode, which is unique not been explored well. Here, we show that melanophilin, one effectors...
Two GATA1-related leukemias have been described: one is an erythroleukemia that develops in mice as a consequence of diminished expression wild-type GATA1, whereas the other acute megakaryoblastic leukemia (AMKL) arises Down syndrome children somatic N-terminal truncation (DeltaNT) GATA1. We discovered expressing shortened GATA1 protein (DeltaNTR mice) phenocopies human transient myeloproliferative disorder (TMD) precedes AMKL children. In perinatal livers DeltaNTR mutant mice, immature...
Although the beneficial effects (e.g., lipid-lowering activity) of γ-oryzanol (OZ), a mixture ferulic acid esters plant sterols and triterpene alcohols, have been extensively investigated, few studies evaluated absorption metabolism OZ. Moreover, it is unclear whether OZ, once ingested, directly absorbed by intestine into bloodstream at sufficient level to exhibit activity. Here, we prepared OZ concentrate from purified rice bran oil (Rice Oil OZ), determined concentration in preparation...
Exophilin-8 has been reported to play a role in anchoring secretory granules within the actin cortex, due its direct binding activities Rab27 on granule membrane and F-actin motor protein, myosin-Va. Here, we show that exophilin-8 accumulates cortical network not by interaction with myosin-Va, but indirect specific form of myosin-VIIa through previously unknown partner, RIM-BP2. RIM-BP2 also associates exocytic machinery, Cav1.3, RIM, Munc13-1. Disruption exophilin-8–RIM-BP2–myosin-VIIa...
γ-Oryzanol (OZ), abundant in rice bran oil, has gained attention due to its physiological activities (e.g., lipid-lowering effects). However, the absorption and metabolism of orally ingested OZ have not yet been fully elucidated. In this study, diets containing normal or high contents were fed obesity model mice for 8 weeks, concentrations plasma organs analyzed by HPLC-MS/MS. To evaluate relationship between accumulation lipid vivo, liver also measured. As a result, intact was seen OZ,...
Transgenic mice expressing a constitutively active form of the aryl hydrocarbon receptor in keratinocytes (AhR-CA mice) develop severe dermatitis that substantially recapitulates pathology human atopic dermatitis. The neurotrophic factor artemin (Artn) is highly expressed epidermis AhR-CA and causes hypersensitivity to itch (alloknesis) by elongating nerves into epidermis. However, whether Artn gene regulated directly AhR or indirectly through complex regulation associated with remains...
Adenosine triphosphate (ATP) is a versatile molecule used mainly for energy and phosphate source. The hydrolysis of γ initiates the reactions these almost always start when ATP binds to protein. Therefore, there should be mechanism prevent spontaneous reaction lead pure source or To address questions, we extensively analyzed effect protein conformation based on sampling solution conformations obtained from molecular dynamics simulation structures bound found in structure database. comparison...