A5041755549- HIV Research and Treatment
- Adipose Tissue and Metabolism
- HIV/AIDS drug development and treatment
- RNA modifications and cancer
- Immune responses and vaccinations
- Protease and Inhibitor Mechanisms
- GDF15 and Related Biomarkers
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- COVID-19 Clinical Research Studies
- Lipid metabolism and disorders
- RNA Research and Splicing
- Diet and metabolism studies
- Endoplasmic Reticulum Stress and Disease
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer, Stress, Anesthesia, and Immune Response
- Immune cells in cancer
- Metabolism, Diabetes, and Cancer
- Nutrition and Health in Aging
- SARS-CoV-2 and COVID-19 Research
Icahn School of Medicine at Mount Sinai
2024
Emory University
2020
IFB Adiposity Diseases
2014
Leipzig University
2011
Abstract The early steps of HIV-1 infection, such as uncoating, reverse transcription, nuclear import, and transport to integration sites are incompletely understood. Here, we imaged entry replication complexes in cell lines, primary monocyte-derived macrophages (MDMs) CD4 + T cells. We show that viral traffic accumulate within speckles these precede the completion DNA synthesis. is dependent on interaction capsid proteins with host cleavage polyadenylation specificity factor 6 (CPSF6),...
An assembly of capsid proteins (CA) form the mature viral core enclosing HIV-1 ribonucleoprotein complex. Discrepant findings have been reported regarding cellular sites and extent disassembly (uncoating) in infected cells. Here, we combined single-virus imaging time-of-drug-addition assays to elucidate kinetic relationship between uncoating, reverse transcription, nuclear import complexes cell lines monocyte-derived macrophages (MDMs). By using cyclophilin A-DsRed (CDR) as a marker for CA,...
Antigenically distinct SARS-CoV-2 variants increase the reinfection risk for vaccinated and previously exposed population due to antibody neutralization escape. COVID-19 severity depends on many variables, including host immune responses, which differ depending genetic predisposition. To address this, we perform profiling of female mice with different backgrounds –transgenic K18-hACE2 wild-type 129S1– infected severe B.1.351, 30 days after exposure milder BA.1 or H1N1. Prior infection...
Abstract MF59 (AddaVax-like) is a clinically approved nanoemulsion adjuvant with an unclear mechanism of action. Here we introduce novel approach to investigating the role muscle regeneration in adjuvanted vaccine response. We found that AddaVax quadrivalent inactivated flu (QIV) induced Th2 immune response IL-4, -5, and -13 expression lungs 5 days after H1N1 lethal challenge. In contrast, IMDQ-PC (TLR7/8 agonist) Th1 lung higher IgG2a titers. BMDM cultures, unlike require IFNγ co-incubation...
Objective: The GRB14 locus (Growth Factor Receptor-Bound Protein 14) has been shown to be associated with waist hip ratio (WHR). To investigate possible mechanisms explaining the association, we assessed relationship between WHR-associated single nucleotide polymorphism (SNP) rs10195252, adipose tissue (AT) mRNA expression and metabolic traits related fat distribution (FD)
Background: Body fat distribution (FD) is one of the main predictors obesity associated complications.
Hintergrund/Fragestellung: Vaspin (visceral adipose tissue derived serine protease inhibitor) ist ein neues Adipokin, dass einen Link zwischen Adipositas, Insulinresistenz (IR) und Typ 2 Diabetes herstellt. Die pathophysiologische Rolle von bis heute jedoch weitgehend unbekannt. Um den Einfluss auf die Insulinsensitivität zu untersuchen, wurden db/db-Mäusen mit rekombinantem behandelt. Weiterhin sollte der Zusammenhang Serumvaspinkonzentrationen metabolischen Merkmalen in humanen Studien...
Background/Aim: Repin1 maps within a quantitative trait locus for total cholesterol, body weight, serum triglycerides and insulin in the rat. Sequencing of human repin1 revealed 12bp deletion coding region (rs3832490). We investigated functional consequences this variant.