A5051540676- Particle Accelerators and Free-Electron Lasers
- Particle accelerators and beam dynamics
- Pluripotent Stem Cells Research
- Health, Medicine and Society
- Superconducting Materials and Applications
- Laser-Plasma Interactions and Diagnostics
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- Renal and related cancers
- Magnetic confinement fusion research
- Particle Detector Development and Performance
- Molecular Biology Techniques and Applications
- Gene Regulatory Network Analysis
- Advanced X-ray Imaging Techniques
- Congenital heart defects research
- Reproductive Biology and Fertility
- Gyrotron and Vacuum Electronics Research
- Pulsed Power Technology Applications
- Nuclear Physics and Applications
- Environmental DNA in Biodiversity Studies
- Forensic and Genetic Research
- Phagocytosis and Immune Regulation
- Zebrafish Biomedical Research Applications
- Radiation Therapy and Dosimetry
- Advanced Surface Polishing Techniques
University of Cambridge
2023-2025
The Francis Crick Institute
2022-2025
Memorial Sloan Kettering Cancer Center
2018-2025
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2006-2023
Institut de Recherche sur les Lois Fondamentales de l'Univers
2016-2023
CEA Paris-Saclay
2007-2023
Université de Nîmes
2023
American Academy of Forensic Sciences
2023
Centre Hospitalier Universitaire de Nîmes
2023
University of Oxford
2017-2021
Our understanding of the molecular events driving cell specification in early mammalian development relies mainly on mouse studies, and it remains unclear whether these mechanisms are conserved across mammals, including humans. We have shown that establishment polarity via aPKC is a event initiation trophectoderm (TE) placental programme mouse, cow human embryos. However, transducing into fate embryos unknown. Here, we examined evolutionary conservation Hippo signalling, which thought to...
Recent advances in single-cell omics have transformed characterisation of cell types challenging-to-study biological contexts. In contexts with limited samples, such as the early human embryo inference transcription factor-gene regulatory network (GRN) interactions is especially difficult. Here, we assessed application different linear or non-linear GRN predictions to simulated and transcriptome datasets. We also compared how expression normalisation impacts on predictions, finding that...
Abstract Studies in the mouse demonstrate importance of fibroblast growth factor (FGF) and extra-cellular receptor tyrosine kinase (ERK) specification embryo-fated epiblast yolk-sac-fated hypoblast cells from uncommitted inner cell mass (ICM) prior to implantation. Molecular mechanisms regulating early lineages human development are comparatively unclear. Here we show that exogenous FGF stimulation leads expanded molecular marker expression, at expense epiblast. Conversely, specifically...
Cell fate decisions in early mammalian embryos are tightly regulated processes crucial for proper development. While FGF signaling plays key roles embryo patterning, its downstream effectors remain poorly understood. Our study demonstrates that the transcription factors Etv4 and Etv5 critical mediators of cell lineage specification maturation mouse embryos. We show loss compromises primitive endoderm formation at pre-implantation stages. Furthermore, Etv4/5 deficiency delays naïve...
SUMMARY Many mammals can control the timing of gestation and birth by pausing embryonic development at blastocyst stage. It is unknown whether capacity to pause conserved, in general across mammals, more specifically humans. Activity growth regulating mTOR pathway governs developmental mouse ( 1 ). Here we show a stage-specific delay progression human via inhibition. In this context, blastoids pluripotent stem cells naïve naïve-like, but not primed, states be induced enter dormant state,...
The T-box transcription factor (TF) Eomes is a key regulator of cell fate decisions during early mouse development. cis-acting regulatory elements that direct expression in the anterior visceral endoderm (AVE), primitive streak (PS) and definitive (DE) have yet to be defined. Here, we identified three gene-proximal enhancer-like sequences (PSE_a, PSE_b VPE) faithfully activate tissue-specific transgenic embryos. However, targeted deletion experiments demonstrate PSE_a are dispensable, only...
Abstract Background Despite their spread in daily practice, few data is available on clinical factors associated with peripherally inserted central catheter (PICC)-related bloodstream infections (PR-BSI). We aimed to assess the PR-BSI incidence, microbiology, and a focus symptoms. Methods conducted retrospective cohort study French university hospital. screened all PICC insertions performed from April 1st, 2018, 2019, included adult patients. assessed using Cox model, negative positive...
Many mammals can temporally uncouple conception from parturition by pacing down their development around the blastocyst stage. In mice, this dormant state is achieved decreasing activity of growth-regulating mTOR signaling pathway. It unknown whether ability conserved in general and humans particular. Here, we show that pathway induces human pluripotent stem cells (hPSCs) blastoids to enter a with limited proliferation, developmental progression, capacity attach endometrial cells. These...
Experiments at the FLASH facility DESY have demonstrated that higher order modes induced in superconducting cavities can be used to provide a variety of beam and cavity diagnostics. The axes determined from orbit produces minimum power dipole HOM modes. phase amplitude obtain high resolution position information, monopole measure relative accelerator rf. For most accelerators, existing mode couplers necessary signals, downmix digitizing electronics are straightforward, similar those for...
The use of peripherally inserted central catheters (PICCs) has increased in cancer patients. This study aimed to compare the incidence PICC-related bloodstream infections (PICCR-BSIs) patients treated with chemotherapy and noncancer We performed a secondary analysis from retrospective, single-center, observational cohort. PICCR-BSI rates were compared after 1:1 propensity-score matching. Then, factors associated assessed Cox model. Among 721 PICCs (627 patients) included analysis, 240 placed...
ABSTRACT The GATA zinc-finger transcription factor GATA4 is expressed in a variety of tissues during mouse embryonic development and adult organs. These include the primitive endoderm blastocyst, visceral early post-implantation embryo, as well lateral plate mesoderm, developing heart, liver, lung gonads. Here, we generate novel Gata4 targeted allele used to both Gata4H2B-GFP transcriptional reporter Gata4FLAG fusion protein analyse dynamic expression domains. We demonstrate that faithfully...
Metals can pose challenges while conducting forensic DNA analysis. The presence of metal ions in evidence-related extracts degrade or inhibit PCR as applied to quantification (real-time qPCR) and/or STR amplification, leading low success profiling. Different were spiked into 0.2 and 0.5 ng human genomic an "inhibition study" the impact was evaluated by qPCR using Quantifiler™ Trio Quantification Kit (Thermo Fisher Scientific) in-house SYBR Green assay. This study reports on a contradictory...
Abstract Recent advances in single-cell-omics have been transformative to characterise cell types challenging study biological contexts. It is technically difficult infer transcription factor-gene regulatory interactions from these datasets, especially contexts with limited single-cell sample number such as the early human embryo. Here we systematically assessed application of four different linear or non-linear gene network (GRN) prediction strategies simulated and embryo transcriptome...
The beam-based alignment and feedback systems, essential operations for the future colliders, require high resolution beam position monitors (BPMs). In framework of European CARE/SRF program, a reentrant cavity BPM with its associated electronics was developed by CEA/DSM/Irfu in collaboration DESY. design, fabrication, test this monitor are detailed within paper. This is designed to be inserted cryomodule, work at cryogenic temperature clean environment. It has achieved better than $10\text{...