We investigated the mechanism of cancer-associated fibroblasts (CAFs) in promoting invasion and metastasis pancreatic cancer cells a non-vascular manner. verified original generation isolated cultured CAFs normal (NFs) based on expression α-SMA vimentin, we examined cell glycolysis level through glucose consumption lactate production experiments. The mRNA protein CAF glycolytic enzymes, dehydrogenase pyruvate kinase m2, were by RT-PCR western blotting, respectively. In vitro culture...

The pluripotent state of embryonic stem cells (ESCs) is defined by its transcriptome and epigenome. chromatin reader Brd4 determines ESC identity. Although regulation in gene transcription has been well described, contribution to the landscape less known. Here, we show that Brd4's bromodomains partner with histone acetyltransferase P300, increasing enzymatic activities. Augmenting acetylation Brd4-P300 interaction recruits remodeler Brg1 altering structure. This pathway important for...

BMI-1 oncogene is a member of the polycomb-group gene family and transcriptional repressor. Overexpression has been identified in various human cancer tissues known to be involved cell proliferation, invasion, distant metastasis, chemosensitivity patient survival. Accumulating evidence revealed that also regulation self-renewal, differentiation tumor initiation stem cells (CSCs). However, molecular mechanisms underlying these biological processes remain unclear. The present review summarized...

Embryonic stem cell (ESC) pluripotency is controlled by defined transcription factors. During cellular differentiation, ESCs undergo a global epigenetic reprogramming. Female exemplify this process as one of the two X-chromosomes globally silenced during X chromosome inactivation (XCI) to balance X-linked gene disparity with XY males. The pluripotent factor OCT4 regulates XCI triggering pairing and counting. directly binds Xite Tsix, which encode long noncoding RNAs (lncRNAs) that suppress...

Abstract Long noncoding RNAs (lncRNAs) are emerging as key regulators of crucial cellular processes. However, the molecular mechanisms many lncRNA functions remain uncharacterized. Sox2ot is an evolutionarily conserved that transcriptionally overlaps pluripotency gene Sox2 , which maintains stemness embryonic stem cells and tissue-specific cells. Here, we show expressed in developing mouse cerebral cortex, where it represses neural progenitor (NP) proliferation promotes neuronal...

Accumulating evidence has shown that micro-RNAs (miRNAs) are involved in multiple processes cancer development and progression. Upregulation of miRNA-494 (miR-494) been identified as an oncogenic miRNA is associated with poor prognosis several types human cancer. However, the specific function miR-494 colorectal remains unclear. In this study we found expression tissues cell lines was much higher than normal control cells, respectively. addition, upregulation more frequently occurred tissue...

Accumulated evidence suggest that specific patterns of histone posttranslational modifications (PTMs) and their crosstalks may determine transcriptional outcomes. However, the regulatory mechanisms these "histone codes" in plants remain largely unknown.In this study, we demonstrate for first time a salinity stress inducible PHD (plant homeodomain) finger domain containing protein GmPHD5 can read code" underlying methylated H3K4. interacts with other DNA binding proteins, including GmGNAT1...

Aims Cancer development and progression is not only associated with the tumor cell proliferation but also depends on interaction between cells stromal microenvironment. A new understanding of role microenvironment suggests that loss caveolin-1 (Cav-1) as a key regulator may become potential therapy target. This study aims to elucidate whether Cav-1 expression in pancreatic cancer can be strong prognosis biomarker. Methods Tissue samples from 45 patients were studied. Parenchyma stroma...

Catalpol is expected to possess diverse pharmacological actions including anti-cancer, anti-inflammatory and hypoglycemic properties. Matrix metalloproteinase-2 (MMP-2) closely related the pathogenesis of ovarian cancer. In addition, microRNA-200 (miR-200) can modulate phenotype, proliferation, infiltration transfer various tumors. Here, OVCAR-3 cells were employed investigate whether effect catalpol (25, 50 100 μg/mL) promoted apoptosis cancer explore potential mechanisms. Our results...

Increasing researches have focused on cancer metastasis and development. The ectonucleotidase CD73 is one of the most common cell surface enzymes that are involved in immunosuppression. In this study, recombinant plasmid pET28a-CD73 was constructed protein overexpressed E. coli as an inclusion body then subjected to refolding. anti-CD73 monoclonal antibody (3F7) obtained by hybridoma technology. subtype identified IgG2a with affinity constant 5.75 nM. This could be applied immunofluorescence...

Abstract Background Histone modifications and histone variants are of importance in many biological processes. To understand the functions global dynamics higher plants, we elucidated post-translational histones soybean, a legume plant with much bigger genome than that Arabidopsis thaliana . Results In soybean leaves, mono-, di- tri-methylation at Lysine 4, 27 36, acetylation 14, 18 23 were detected HISTONE H3. was prone to being mono-methylated, while predominant 36. We also observed...

A growing number of studies have shown the prognostic importance Cell division cycle protein 45 (CDC45) in hepatocellular carcinoma (HCC). This study aims to investigate biological function and mechanism CDC45 HCC. The differential expression significance HCC normal tissues were analyzed by bioinformatics. was knocked down effects vitro vivo measured. Subsequently, using RNA m6A colorimetry Methylated Immunoprecipitation (MeRIP), levels modification total evaluated cells. RIP applied...

Abstract Multidrug resistance ( MDR ) often leads to chemotherapy failure of lung cancer and has been linking the cellular expression several DNA transcription‐ repair‐related genes such as Trps1 MGMT . However, their roles in formation are largely unknown. In this study, overexpression/knockdown, luciferase assay Ch IP were performed study relationship between , well formation. Our results demonstrated that both increased drug‐resistant cell line (H446/ CDDP ). Silencing resulted...

Abstract Background Neuronal activity-induced changes in gene expression patterns are important mediators of neuronal plasticity. Many genes can be activated or inactivated response to depolarization. Mechanisms that activate transcription well established, but activity-dependent mechanisms silence less understood. It is also not clear what the significance inhibiting these during activity. Methods Quantitative Real Time-PCR, western blot and immunofluorescence staining were performed...

ABSTRACT The GATA zinc-finger transcription factor GATA4 is expressed in a variety of tissues during mouse embryonic development and adult organs. These include the primitive endoderm blastocyst, visceral early post-implantation embryo, as well lateral plate mesoderm, developing heart, liver, lung gonads. Here, we generate novel Gata4 targeted allele used to both Gata4H2B-GFP transcriptional reporter Gata4FLAG fusion protein analyse dynamic expression domains. We demonstrate that faithfully...

Neuropilin-1 (NRP-1), a member of the NRP-family, has been reported to be vital for tumor angiogenesis, growth and metastasis. As co-receptor vascular endothelial factor (VEGF), NRP-1 can bind VEGF meditate development through VEGF-VEGF receptor 2 (VEGFR2) signaling pathway. Furthermore, is capable binding with platelet-derived (PDGF) regulate PDGF-PDGF (PDGR) pathway in angiogenesis. In present study, The DNA was obtained from paraffin-embedded tissues patients advanced gastric cancer...

Abstract Gastric cancer is the fifth most common and third leading cause of cancer-related mortality globally. Abnormal DNA methylation closely related to gastric cancer. The purpose study was investigate SYNE1 MAGI2 gene promoter its relationship with clinical-pathological factors, chemotherapy efficacy, survival, thus providing a new biomarker for prognosis efficacy in status adjacent tissues detected by MSP method 70 cases advanced paraffin specimens. rate region higher compared tissues....