A5001480829- Electrospun Nanofibers in Biomedical Applications
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Wound Healing and Treatments
- Pancreatic function and diabetes
- Liver physiology and pathology
- Hydrogels: synthesis, properties, applications
- Reconstructive Surgery and Microvascular Techniques
- Angiogenesis and VEGF in Cancer
- Cardiac Ischemia and Reperfusion
- Advanced Drug Delivery Systems
- Body Contouring and Surgery
- Elbow and Forearm Trauma Treatment
- Orthopedic Surgery and Rehabilitation
- Polymer Surface Interaction Studies
- Adipose Tissue and Metabolism
- RNA Interference and Gene Delivery
- Graphene and Nanomaterials Applications
- Diabetes Management and Research
- Silk-based biomaterials and applications
- Phagocytosis and Immune Regulation
- Cardiac and Coronary Surgery Techniques
- Antimicrobial agents and applications
O'Brien Institute
2012-2024
St Vincents Institute of Medical Research
2017-2024
Centre for Eye Research Australia
2022
St Vincent's Hospital
2005-2020
The University of Melbourne
2005-2020
Australian Catholic University
2012-2019
University of Bergen
2019
Murdoch Children's Research Institute
2018
St. Vincent's Birmingham
2011
Background: Bacterial infection is a common and serious complication in orthopedic implants following traumatic injury, which often associated with extensive soft tissue damage contaminated wounds. Multidrug-resistant bacteria have been found these infected wounds, especially patients who multi trauma prolonged stay intensive care units.Purpose: The objective of this study was to develop coating on that effective against drug-resistant bacteria. Methods results: We applied nanoparticles...
We have previously described a mouse adipose tissue engineering model using silicon chamber enclosing the superficial epigastric pedicle in Matrigel based environment. shown that when Zymosan, sterile inflammatory agent, is added to chamber, angiogenesis and adipogenesis are significantly improved. As Zymosan interacts with toll-like receptors on macrophages, role of macrophages new development was assessed. Morphological histological results showed were presenting high numbers at 2 weeks...
We have recently shown that Matrigel-filled chambers containing fibroblast growth factor-2 (FGF2) and placed around an epigastric pedicle in the mouse were highly adipogenic. Contact of this construct with pre-existing tissue or a free adipose graft was required. To further investigate mechanisms underpinning formation new tissue, we seeded these human biopsies adipose-derived cell populations severe combined immunodeficient mice assessed origin resultant after 6 weeks using species-specific...
Tissue engineering and cell implantation therapies are gaining popularity because of their potential to repair regenerate tissues organs. To investigate the role inflammatory cytokines in new tissue development engineered tissues, we have characterized nature timing populations forming adipose a mouse chamber (TEC) gene protein expression newly developing tissues. EGFP-labeled bone marrow transplant mice MacGreen were implanted with TEC for periods ranging from 0.5 days 6 weeks. Tissues...
In in vivo tissue engineering, many implanted cells die because of hypoxic conditions immediately postimplantation. The aim this study was to determine whether delayed myoblast implantation, at day 4 or 7, improves survival compared with implantation 0 an arterio-venous loop (AB loop) chamber model. adult inbred Sprague-Dawley rats, AB inserted into a plastic (day 0). Group I, 0, two million DiI-labeled (neonatal inbred) myoblasts were around the loop. Groups II and III, created novel cell...
Cell constructs have been utilized as building blocks in tissue engineering to closely mimic the natural and also overcome some of limitations caused by two-dimensional cultures or using scaffolds. External forces can be used enhance cells' adhesion interaction thus provide better control over production these structures compared methods like cell seeding migration. In this paper, we demonstrate an efficient method generate uniform, three-dimensional magnetic forces. This produced spheroids...
Macrophages predominate among the cells that directly interact with biomaterials and are key orchestrators of host-biomaterial interactions. However, macrophage response to synthetic scaffolds in particular has not been well studied. The aim this study was therefore characterise several rat using immunohistological techniques for a panel markers subclass or activation, including ED1 (CD68), ED2 (CD163), CD80, mannose receptor inducible nitric oxide synthase (iNOS). Materials were implanted...
Background Inducible nitric oxide synthase (nitric 2, NOS 2) inhibition significantly suppresses chronically ischaemic skin flap survival, possibly because of reduced angiogenesis. Objectives To investigate the effect genetic 2 on cutaneous wound angiogenesis in two vivo murine models. The impact manipulation vascular endothelial growth factor (VEGF)‐A stimulated and fibroblast (FGF)‐2 was also investigated Matrigel® plug assay. Methods (i) Matrigel plugs/incisional wounds: groups 2−/− mice...
Tissue engineering is a complex and dynamic process that requires varied biomolecular cues to promote optimal tissue growth. Consequently, the development of delivery systems capable sequestering more than one biomolecule with controllable release profiles key step in advancement this field. This study develops multilayered polyelectrolyte films incorporating alpha-melanocyte stimulating hormone (α-MSH), an anti-inflammatory molecule, basic fibroblast growth factor (bFGF). The layers were...
Free fat grafting is popular, but it still unclear how works. Although focusing on graft survival seems an obvious direction for improving clinical results, the authors' research suggests that long-term volume retention in part attributable to new regeneration. Measures facilitate adipogenesis may therefore be equally important.To investigate relative roles of and regeneration grafts, authors measured fate human lipoaspirate implanted into scalps immunodeficient mice, with without stromal...
Recent studies have shown that type 1 diabetes can be reversed in a murine model by islet transplantation to vascularized tissue engineering chamber. In preliminary experiments using prevascularized chamber we observed grafts not functioning initially show delayed onset of function several weeks after implantation. We sought characterize this phenomenon.Islets were transplanted into chambers based on the epigastric vessels streptozotocin induced diabetic C57BL/6J mice. Animals with 500...
Many biomaterials used in tissue engineering cause a foreign body response vivo, which left untreated can severely reduce the effectiveness of regeneration. In this study, an anti-inflammatory hormone α-melanocyte stimulating (α-MSH) was physically adsorbed to surface biodegradable poly (lactic-co-glycolic) acid (PLGA) microspheres inflammatory responses material. The stability and adsorption isotherm peptide binding were characterized. secondary structure not perturbed by subsequent...
Nonvascularized fat grafting is a valuable technique for soft tissue reconstruction but poor survival of in the host environment remains problem. A process known as cell-assisted transfer used to enhance graft retention by adding stromal vascular fraction, an adipose-derived stem cell (ASC) rich content lipoaspirate. We have recently shown that use melatonin, reactive oxygen species scavenger, protects human ASCs from hydrogen peroxide-induced oxidative stress and death vitro its role...
Current cell-based treatment alternatives to organ transplantation for liver failure remain unsatisfactory. Hepatocytes have a strong tendency dedifferentiate and apoptose when isolated maintained in culture. In contrast, progenitor cells (LPCs) are robust, easy culture been shown replace damaged hepatocytes disease. this study we investigate whether LPCs can survive differentiate toward mature vivo implanted into heterotopic mouse tissue engineering chamber model. Healthy Balb/c mice those...