A5080152929- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Pneumocystis jirovecii pneumonia detection and treatment
- Biosensors and Analytical Detection
- vaccines and immunoinformatics approaches
- Advanced Biosensing Techniques and Applications
- Infectious Diseases and Tuberculosis
- Bacterial Genetics and Biotechnology
- Pneumonia and Respiratory Infections
- Diagnosis and treatment of tuberculosis
Chinese Center For Disease Control and Prevention
2022-2025
Diabetes mellitus (DM) is a major risk factor for tuberculosis (TB), However, limited research exists on their clinical and strain characteristics. This study aims to investigate the correlation between these factors in TB-DM patients Changping District. Whole genome sequencing (WGS) drug susceptibility tests (DST) were performed culture-positive strains. Spearman analysis was used examine lineage, cavities, hemoptysis population. The specificity, sensitivity, confidence intervals predicting...
Introduction A new class of Mycobacterium tuberculosis (MTB) antigen-based skin tests was recommended by WHO for the diagnosis TB infection. However, their performance in some settings remains suboptimal. Our study focused on screening novel MTB recombinant antigens and evaluating preclinical efficacy infection detection. Methods We constructed Escherichia coli BL21 expression vectors to produce a series antigens. assessed ability detect through tests. Model animals sensitized with BCG were...
Abstract Background Pretomanid is a key component of new regimens for the treatment drug-resistant tuberculosis (TB) which are being rolled out globally. However, there limited information on prevalence pre-existing resistance to drug. Methods To investigate pretomanid rates in China and its underlying genetic basis, as well generate additional minimum inhibitory concentration (MIC) data epidemiological cutoff (ECOFF)/breakpoint setting, we performed MIC determinations Mycobacterial Growth...
Introduction Early and accurate diagnosis of tuberculosis (TB) is crucial for initiating timely treatment preventing new infections. In this study, we introduced the iFIND TBR assay, an automated all-in-one detection approach that simultaneously detect Mycobacterium (MTB) rifampicin (RIF) resistance. Methods The limits (LOD), sensitivity, specificity, RIF-R rpoB mutation were tested on DNA or sputum samples spiked with known numbers M.tuberculosis H37Rv. Frozen clinical from patients...
Early diagnosis of drug susceptibility for tuberculosis (TB) patients could guide the timely initiation effective treatment. We evaluated a novel multiplex xMAP TIER (Tuberculosis-Isoniazid-Ethambutol-Rifampicin) assay based on Luminex system to detect first-line anti-tuberculous resistance. Deoxyribonucleic acid samples from 353 Mycobacterium clinical isolates were amplified by polymerase chain reaction, followed hybridization and analysis through system. Compared with broth microdilution...
Mycobacterial membrane proteins play a pivotal role in the bacterial invasion of host cells; however, precise mechanisms underlying certain remain elusive. Mycolicibacterium smegmatis (Ms) msmeg5257 is hemolysin III family protein that homologous to Mycobacterium tuberculosis (Mtb) Rv1085c, but it has an unclear function growth. To address this issue, we utilized CRISPR/Cas9 gene editor construct Δmsmeg5257 strains and combined RNA transcription LC-MS/MS profiling determine functional Ms The...