A5014179463- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- PI3K/AKT/mTOR signaling in cancer
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
- Fungal Plant Pathogen Control
- Genomics and Phylogenetic Studies
- Plant Virus Research Studies
- Insect Resistance and Genetics
- Microbial Metabolic Engineering and Bioproduction
- RNA and protein synthesis mechanisms
- Algal biology and biofuel production
- Microbial Community Ecology and Physiology
- Plant Stress Responses and Tolerance
- Protist diversity and phylogeny
- Legume Nitrogen Fixing Symbiosis
- Polysaccharides and Plant Cell Walls
- Mitochondrial Function and Pathology
- Plant Pathogens and Resistance
- Potato Plant Research
- Light effects on plants
- Plant Surface Properties and Treatments
- Polyamine Metabolism and Applications
Centre National de la Recherche Scientifique
2004-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2011-2024
Aix-Marseille Université
2009-2024
Institut de Biosciences et Biotechnologies
2011-2024
CEA Cadarache
2002-2023
John Innes Centre
2004-2017
Institute of Environmental Biology and Biotechnology
2010-2015
Ecologie Microbienne Lyon
2015
Zhejiang University
2007
State Key Laboratory of Plant Physiology and Biochemistry
2007
TOR (target of rapamycin) protein kinases were identified in yeasts, mammals, and Drosophila as central controllers cell growth response to nutrient factors. Here we show that Arabidopsis thaliana possesses a single gene encoding able complex with yeast 12-kDa FK506-binding rapamycin despite the insensitivity vegetative rapamycin. Analysis two T-DNA insertion mutants shows disruption AtTOR leads premature arrest endosperm embryo development. A T-DNA-mediated translational fusion GUS reporter...
Root hairs and rhizoids are cells with rooting functions in land plants. We describe two basic helix-loop-helix transcription factors that control root hair development the sporophyte (2n) of angiosperm Arabidopsis thaliana rhizoid gametophytes (n) bryophyte Physcomitrella patens. The phylogeny plants supports hypothesis early were bryophyte-like possessed a dominant gametophyte later rose to dominance. If this is correct, our data suggest increase morphological complexity body Paleozoic...
Summary An Arabidopsis thaliana line bearing a transposon insertion in the gene coding for isozyme form of plant‐specific cap‐binding protein, eukaryotic initiation factor (iso) 4E (eIF 4E), has been isolated. This mutant completely lacks both eIF(iso)4E mRNA and but was found to have phenotype fertility indistinguishable from wild‐type plants under standard laboratory conditions. In contrast, amount related eIF4E protein increase seedling extracts. Furthermore, polysome analysis shows that...
The TOR (target of rapamycin) protein, a large phosphatidylinositol 3-kinase-like protein kinase (PIKK) that is conserved in eukaryotes and central regulator growth metabolism. analysis function plant development has been limited by the fact plants are very poorly sensitive to rapamycin. As domain highly conserved, this study analysed dose-dependent effect three sets first- second-generation ATP-competitive inhibitors (called asTORis for active-site inhibitors) recently developed human on...
The evolution of multicellular organisms was made possible by the underlying gene regulatory networks. In animals, core networks consists kernels, stable subnetworks transcription factors that are highly conserved in distantly related species. However, plants it is not clear when and how kernels evolved. We show here RSL (ROOT HAIR DEFECTIVE SIX-LIKE) form an ancient land plant kernel controlling caulonema differentiation moss Physcomitrella patens root hair development flowering Arabidopsis...
Tip growth is a mode of cell expansion in which all restricted to small area that forms tip an elongating cell. In green plants, has been shown occur root hairs, pollen tubes, rhizoids, and caulonema. Each these types longitudinally elongated shape, oriented microtubules actin microfilaments, characteristic cytoplasmic organization at the growing required for growth. Chloronema are cylindrical shaped cells form during development moss protonema. Since there no published reports on precise...
Abstract Background The eukaryotic TOR pathway controls translation, growth and the cell cycle in response to environmental signals such as nutrients or growth-stimulating factors. protein kinase can be inactivated by antibiotic rapamycin following formation of a ternary complex between TOR, FKBP12 proteins. is also found higher plants despite fact that they are insensitive. Previous findings using yeast two hybrid system suggest plant homolog unable form with while FRB domain still able...
Land plants are anchored to their substratum from which essential inorganic nutrients taken up. These functions carried out by a system of rhizoids in early diverging groups land plants, such as mosses, liverworts and hornworts. Physcomitrella patens RHD SIX-LIKE1 (PpRSL1) PpRSL2 transcription factors necessary for rhizoid development mosses. Similar proteins, AtRHD6 AtRSL1, control the root hairs Arabidopsis thaliana. Auxin positively regulates hair independently AtRSL1 A. thaliana but...
ABSTRACT TARGET OF RAPAMYCIN (TOR) is a conserved eukaryotic phosphatidylinositol-3-kinase-related kinase that plays major role in regulating growth and metabolism response to environment plants. We performed genetic screen for Arabidopsis ethylmethane sulfonate mutants resistant the ATP-competitive TOR inhibitor AZD-8055 identify new components of plant pathway. found loss-of-function DYRK (dual specificity tyrosine phosphorylation regulated kinase)/YAK1 are and, reciprocally, YAK1...
One-fifth of the tRNAs used in plant mitochondrial translation is coded for by chloroplast-derived tRNA genes. To understand how aminoacyl–tRNA synthetases have adapted to presence these mitochondria, we cloned an Arabidopsis thaliana cDNA coding a methionyl–tRNA synthetase. This enzyme was chosen because chloroplast-like elongator Met genes been described several species, including A. . We demonstrate here that isolated codes both chloroplastic and synthetase (MetRS). The protein...
Potyviruses are important plant pathogens that rely on many cellular processes for successful infection. TOR (target of rapamycin) signalling is a key eukaryotic energy-signalling pathway controlling such as translation and autophagy. The dependence potyviruses active was examined. Arabidopsis lines downregulated by RNAi were challenged with the watermelon mosaic virus (WMV) turnip (TuMV). WMV accumulation found to be severely altered while TuMV only slightly delayed. In another approach,...
Chloroplasts are the powerhouse of plant cell, and their activity must be matched to growth avoid photooxidative damage. We have identified a posttranslational mechanism linking eukaryotic target rapamycin (TOR) kinase that promotes guanosine tetraphosphate (ppGpp) signaling pathway prokaryotic origins regulates chloroplast photosynthesis in particular. find RelA SpoT homolog 3 (RSH3), nuclear-encoded enzyme responsible for ppGpp biosynthesis, interacts directly with TOR complex via...
We characterized two Arabidopsis thaliana cDNAs coding for class I valyl‐tRNA synthetase and II threonyl‐tRNA synthetase. The proteins display characteristics of cytosolic enzymes, yet possess an N‐terminal extension relative to their prokaryotic homologs. proximal part the is a mitochondrial‐targeting signal. Through transient expression GFP fusions in tobacco cells, we demonstrated that both genes encode mitochondrial forms enzymes by alternative use in‐frame initiation codons. A long,...
Lipid-specific live cell dyes are an important tool for the study of algal lipid metabolism, monitoring production, and identification strains with high yields. Nile Red BODIPY have emerged as principal these purposes. However, they suffer from a number shortcomings including specificity, penetration, interference chlorophyll autofluorescence, incompatibility widely used genetically encoded reporters in green blue regions spectrum such fluorescent protein red protein. We tested new dye,...
Chloroplasts retain elements of a bacterial stress response pathway that is mediated by the signalling nucleotides guanosine penta- and tetraphosphate ((p)ppGpp). In model flowering plant Arabidopsis, ppGpp acts as potent regulator plastid gene expression influences photosynthesis, growth development. However, little known about metabolism or its evolution in other photosynthetic eukaryotes. Here, we studied function diatom Phaeodactylum tricornutum using transgenic lines containing an...
Abstract The nucleotides guanosine tetraphosphate and pentaphosphate (together known as (p)ppGpp or magic spot) are produced in plant plastids from GDP/GTP ATP by RelA-SpoT homologue (RSH) enzymes. In the model Arabidopsis regulates chloroplast transcription translation to affect growth, is also implicated acclimation stress. However, little about metabolism its evolution other photosynthetic eukaryotes. Here we studied marine diatom Phaeodactylum tricornutum . We identified three expressed...