A5090958757- Photosynthetic Processes and Mechanisms
- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Algal biology and biofuel production
- Mitochondrial Function and Pathology
- Biochemical and Molecular Research
- Mass Spectrometry Techniques and Applications
- Microbial Metabolic Engineering and Bioproduction
- Vector-borne infectious diseases
- Genomics and Phylogenetic Studies
- Lysosomal Storage Disorders Research
- Diatoms and Algae Research
- Metalloenzymes and iron-sulfur proteins
- Microplastics and Plastic Pollution
- Protist diversity and phylogeny
- Marine and coastal ecosystems
- Microbial Community Ecology and Physiology
- Parasites and Host Interactions
- Biochemical and Structural Characterization
- Mosquito-borne diseases and control
- Microbial Fuel Cells and Bioremediation
- Metabolism and Genetic Disorders
- Biofuel production and bioconversion
- Parasitic Infections and Diagnostics
- Streptococcal Infections and Treatments
Aix-Marseille Université
2011-2023
Centre National de la Recherche Scientifique
2011-2023
University of Portsmouth
2021-2023
Bioénergétique et Ingénierie des Protéines
2007-2020
University of the Andes
2007-2017
Universidad de Los Andes
2009-2016
Institut de Microbiologie de la Méditerranée
2012
Universidad de Los Andes
1997-2012
In-Q-Tel
2011
Sorbonne Université
1998-2000
Abstract Enzymatic deconstruction of poly(ethylene terephthalate) (PET) is under intense investigation, given the ability hydrolase enzymes to depolymerize PET its constituent monomers near polymer glass transition temperature. To date, reported hydrolases have been sourced from a relatively narrow sequence space. Here, we identify additional PET-active biocatalysts natural diversity by using bioinformatics and machine learning mine 74 putative thermotolerant hydrolases. We successfully...
Glycolysis and glyconeogenesis play crucial roles in the ATP supply synthesis of glycoconjugates, important for viability virulence, respectively, human-pathogenic stages Trypanosoma brucei, cruzi, Leishmania spp. These pathways are, therefore, candidate targets antiparasite drugs. The glycolytic/gluconeogenic enzyme enolase is generally highly conserved, with similar overall fold identical catalytic residues all organisms. Nonetheless, potentially differences exist between trypanosomatid...
Enzyme-based depolymerization is a viable approach for recycling of poly(ethylene terephthalate) (PET). PETase from Ideonella sakaiensis (IsPETase) capable PET hydrolysis under mild conditions but suffers concentration-dependent inhibition. In this study, inhibition found to be dependent on incubation time, the solution conditions, and surface area. Furthermore, evident in other mesophilic PET-degrading enzymes varying degrees, independent level activity. The has no clear structural basis,...
Possible binding proteins of CP12 in a green alga, Chlamydomonas reinhardtii, were investigated. We covalently immobilized on resin and then used it to trap partners. Thus, we found an association between phosphoribulokinase (EC 2.7.1.19), glyceraldehyde 3-phosphate dehydrogenase 1.2.1.13) aldolase. Immunoprecipitation with purified antibodies supported these data. The dissociation constant fructose 1,6-bisphosphate 4.1.2.13) aldolase was measured by surface plasmon resonance is equal 0.48...
A bienzyme complex made up of phosphoribulokinase and glyceraldehyde-3-phosphate dehydrogenase has been isolated purified from chloroplasts Chlamydomonas reinhardtii. The contains four eight polypeptide chains. As is dimeric tetrameric, it concluded that the comprises two molecules. Its overall molecular mass 460 kDa, which in excellent agreement with its stoichiometry. Moreover, owing to nature enzymes, this must catalyse nonconsecutive reactions. tended spontaneously dissociate into free...
CP12, a member of the intrinsically disordered protein family, forms stable complex with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and phosphoribulokinase (PRK). To understand function conserved residues CP12 in formation GAPDH–CP12–PRK regulation enzymes within this complex, we have produced mutants by site-directed mutagenesis. The GAPDH, PRK recombinant proteins are able to reconstitute spontaneously ternary that has been described Chlamydomonas reinhardtii. Our analysis reveals...
Summary Diatoms are a widespread and ecologically important group of heterokont algae that contribute c . 20% to global productivity. Previous work has shown regulation their key C alvin cycle enzymes differs from the Plantae, in crude extracts, glyceraldehyde‐3‐phosphate dehydrogenase ( GAPDH ) can be inhibited by nicotinamide adenine dinucleotide phosphate reduced NADPH under oxidizing conditions. The freshwater diatom, A sterionella formosa , was studied using enzyme kinetics,...
Oxidized, free, stable phosphoribulokinase from Chlamydomonas reinhardtii was almost completely devoid of catalytic activity (0.06 s(-1)/site). However, when it bound to glyceraldehyde-3-phosphate dehydrogenase the same organism, displayed significant (3.25 Moreover, this complex tended spontaneously dissociate upon dilution; isolated increased up 56 s(-1)/site, subsequently decreased, and finally became inactive. Its intrinsic kinetic properties (Km k(cat)) changed with variation overall...
Chloroplasts retain elements of a bacterial stress response pathway that is mediated by the signalling nucleotides guanosine penta- and tetraphosphate ((p)ppGpp). In model flowering plant Arabidopsis, ppGpp acts as potent regulator plastid gene expression influences photosynthesis, growth development. However, little known about metabolism or its evolution in other photosynthetic eukaryotes. Here, we studied function diatom Phaeodactylum tricornutum using transgenic lines containing an...