A5024887220- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- DNA Repair Mechanisms
- Chromosomal and Genetic Variations
- Immune Cell Function and Interaction
- Hearing, Cochlea, Tinnitus, Genetics
- Connexins and lens biology
- Genomic variations and chromosomal abnormalities
- Prenatal Screening and Diagnostics
- Cancer Cells and Metastasis
- Hematopoietic Stem Cell Transplantation
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Corneal Surgery and Treatments
- Immune Response and Inflammation
- Effects of Radiation Exposure
- Metabolism and Genetic Disorders
- Molecular Biology Techniques and Applications
- Lung Cancer Treatments and Mutations
- Acute Lymphoblastic Leukemia research
- Animal Genetics and Reproduction
- Cancer Genomics and Diagnostics
- Pluripotent Stem Cells Research
Institute of Cytology and Genetics
2015-2025
Novosibirsk State University
2020-2025
Siberian Branch of the Russian Academy of Sciences
2014-2025
Immanuel Kant Baltic Federal University
2016-2019
Russian Academy of Sciences
2009-2013
Pathogenic variants in the SLC26A4 gene (OMIM #605646), leading to non-syndromic recessive hearing loss type 4 (DFNB4) and Pendred syndrome, significantly contribute etiology of many populations world. The spectrum prevalence different pathogenic are characterized by wide ethno-geographical variability. A high frequency some them certain regions world may indicate either their independent origin or be a consequence founder effect. proportion SLC26A4-associated Tuvinian patients (the Tyva...
Abstract Background Immunization of mice with tumor homogenate after combined treatment cyclophosphamide (CP) and double-stranded DNA (dsDNA) preparation is effective at inhibition growth challenged the treatment. It was assumed that this might be due to activation antigen-presenting cells. The purpose develop improved antitumor strategy using mice. We studied action cytostatics doxorubicin (Dox) plus CP subsequent dsDNA on growth. Methods Three-month old CBA/Lac were used in experiments....
Heat shock gene promoters represent a highly conserved and universal system for the rapid induction of transcription after various stressful stimuli. We chose pairs mammalian insect species that significantly differ in their thermoresistance constitutive levels Hsp70 to compare hsp promoter strength under normal conditions heat (HS). The first pair includes HSPA1 camel (Camelus dromedarius) humans. It was demonstrated HSPA1A HSPA1L function normally vitro human cell cultures exceed...
Abstract Background When cyclophosphamide and preparations of fragmented exogenous genomic double stranded DNA were administered in sequence, the regressive effect on tumor was synergic: this combined treatment had a more pronounced than alone. Our further studies demonstrated that stimulated maturation specific activities dendritic cells. This suggests cyclophosphamide, with DNA, leads to an immune response tumors grafted into subjects post treatment. Methods Three-month old CBA/Lac mice...
Pathogenic variants in the SLC26A4 gene leading to nonsyndromic recessive deafness (DFNB4), or Pendred syndrome, are some of most common causes hearing loss worldwide. Earlier, we found a high proportion SLC26A4-related with prevailing pathogenic variant c.919-2A>G (69.3% among all mutated alleles that have been identified) Tuvinian patients belonging indigenous Turkic-speaking Siberian people living Tyva Republic (Southern Siberia, Russia), which implies founder effect accumulation...
Mutations in the GJB2 gene encoding transmembrane protein connexin 26 (Cx26) are most common cause for hearing loss worldwide. Cx26 plays a crucial role ionic and metabolic homeostasis inner ear, indispensable normal process. Different pathogenic mutations can affect all stages of life cycle result nonsyndromic autosomal recessive (DFNB1) or dominant (DFNA3) deafness syndromes associating with skin disorders. This study aims to elucidate functional consequences rare variant c.516G>C...
We performed a multicenter, double-blind, placebo-controlled, phase II clinical trial of human dsDNA-based preparation Panagen in tablet form. In total, 80 female patients with stage II-IV breast cancer were recruited.Patients received three consecutive FAC (5-fluorouracil, doxorubicin and cyclophosphamide) or AC (doxorubicin adjuvant chemotherapies (3 weeks per course) 6 tablets 5 mg placebo daily (one every 2-3 hours, 30 mg/day) for 18 days during each chemotherapy course. Statistical...
We report on the results of a phase II clinical trial Panagen (tablet form fragmented human DNA preparation) in breast cancer patients (placebo group n = 23, 57). was administered as an adjuvant leukoprotective agent FAC and AC chemotherapy regimens. Pre-clinical studies clearly indicate that acts by activating dendritic cells induces development adaptive anticancer immune response. analyzed 5-year disease-free survival recruited into trial. Five-year placebo 40 % (n 15), compared with arm –...
X chromosome inactivation takes place in the early development of female mammals and depends on Xist gene expression. The mechanisms expression regulation have not been well understood so far. In this work, we compared promoter region vole Microtus rossiaemeridionalis other mammalian species. We observed three conserved regions which were characterized by computational analysis, DNaseI vitro footprinting, reporter construct assay. Regulatory factors potentially involved activation repression...
Abstract Neuronal tracing is a modern technology that based on the expression of fluorescent proteins under control cell type–specific promoters. However, random genomic integration reporter construct often leads to incorrect spatial and temporal marker protein. Targeted (or knock‐in) coding sequence supposed provide better by exploiting endogenous regulatory elements. Here we describe generation two systems: enhanced green protein (EGFP) pan‐neural class III β‐tubulin ( Tubb3 ) promoter...
Primary cultures of human glioblastoma were obtained from the surgical material patients K. (female, 61 years, Ds: relapse glioblastoma) and Zh. 60 glioblastoma). The effectiveness a new therapeutic approach aimed at destroying cancer cell community was evaluated on primary lines culture by employing strategy tumor-initiating stem synchronization domestic their eradication "3+1". key elements following indicator results: (1) evaluation presence cells in population analyzed ability to...
For medical genetic counseling, estimating the chance of a child being born with chromosome abnormality is crucially important. Cytogenetic diagnostics parents balanced karyotype are special case. Such rearrangements cannot be detected comprehensive screening. In current paper, we consider in two cases rearrangement patients and provide results detailed analysis complex chromosomal (CCR) involving three chromosomes small supernumerary marker (sSMC) patient impaired reproductive function. The...