A5064324022- Immunotherapy and Immune Responses
- Glioma Diagnosis and Treatment
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- T-cell and B-cell Immunology
- Traumatic Brain Injury and Neurovascular Disturbances
- Cancer Cells and Metastasis
- Immune Response and Inflammation
- Cancer Research and Treatments
- Anatomy and Medical Technology
- Virus-based gene therapy research
- Cerebrospinal fluid and hydrocephalus
- Medical Imaging and Analysis
- MicroRNA in disease regulation
- Metabolomics and Mass Spectrometry Studies
- Spinal Dysraphism and Malformations
- Cell Adhesion Molecules Research
- Facial Trauma and Fracture Management
- 3D Printing in Biomedical Research
- Dental Radiography and Imaging
- Cancer Immunotherapy and Biomarkers
- Head and Neck Surgical Oncology
- Bone Tissue Engineering Materials
- Artificial Intelligence in Healthcare and Education
- Additive Manufacturing and 3D Printing Technologies
Novosibirsk Scientific Research Institute of Traumatology and Orthopedics. Ya.L. Tsivyan
2016-2025
Ministry of Health of the Russian Federation
2020-2025
Cellular Research (United States)
2020
Russian Academy of Sciences
2020
Research Institute of Fundamental and Clinical Immunology
2020
It has been established previously that up to 40% of mouse CD34+ hematopoietic stem cells are capable internalizing exogenous dsDNA fragments both in vivo and ex vivo. Importantly, when mice treated with a combination cyclophosphamide dsDNA, the repair interstrand crosslinks progenitors is attenuated, their pluripotency altered. Here we show for first time among various actively proliferating mammalian cell populations there subpopulations fragments. In context cancer, such...
The metabolomic profiles of glioblastoma and surrounding brain tissue, comprising 17 samples 15 peritumoral tissue samples, were thoroughly analyzed in this investigation. LC-MS/MS method was used to analyze over 400 metabolites, revealing significant variations metabolite content between tumor tissues. Statistical analyses, including the Mann–Whitney Cucconi tests, identified several particularly ceramides, that showed differences Pathway analysis using KEGG database, conducted with...
Virotherapy is one of the perspective technologies in treatment malignant neoplasms. Previously, we have developed oncolytic vaccinia virus VV-GMCSF-Lact and its high cytotoxic activity antitumor efficacy against glioma was shown. In this work, using immortalized patient-derived cells with different sensitivity to VV-GMCSF-Lact, evaluated effect chemotherapy agents. Additionally, studied combination temozolomide which most preferred drug for treatment. Experimental results indicate that...
This work compares the metabolic profiles of plasma and cerebrospinal fluid (CSF) patients with high-grade (III IV) gliomas conditionally healthy controls using wide-range targeted screening low molecular metabolites by HPLC-MS/MS. The obtained data were analyzed robust linear regression Huber’s M-estimates, a number correlated content in CSF was identified. statistical analysis shows significant correlation metabolite samples for majority metabolites. Several shown to have high control...
Glioblastoma is one of the most aggressive brain tumors. Given poor prognosis this disease, novel methods for glioblastoma treatment are needed. Virotherapy actively developed approaches cancer therapy today. VV-GMCSF-Lact a recombinant vaccinia virus with deletions viral thymidine kinase and growth factor genes insertions granulocyte-macrophage colony-stimulating oncotoxic protein lactaptin genes. The has high cytotoxic activity against human cells various histogenesis antitumor efficacy...
Glioma is the most common and heterogeneous primary brain tumor. The development of a new relevant preclinical models necessary. As research moves from cultures adherent gliomas to more model, neurospheres, it necessary understand changes that cells undergo at transcriptome level. In present work, we used three patient-derived two immortalized glioblastomas, while their cultivation was carried out under culture neurosphere (NS) conditions. When comparing transcriptomes monolayer (ML) NS cell...
Poorly differentiated cell populations including tumor-initiating stem cells have been demonstrated to display a unique ability natively internalize fragmented double-stranded DNA. Using this feature as marker, we show that 0.1% 6% of human glioblastoma from the bioptates can effectively fluorescently labeled DNA probe. Of these, using samples 3 patients, 66% 100% are also positive for CD133, well-established surface marker glioma cells. primary malignant brain lesions (33 patients),...
Besides initiation of tumor-specific T cell immunity, dendritic cells (DCs) are endowed with tumoricidal activity. Previously, we showed that monocyte-derived DCs high-grade glioma patients generated in the presence interferon alpha (IFNα) (IFN-DCs) have impaired cytotoxic activity against tumor necrosis factor (TNFα)-sensitive HEp-2 cells. Herein, demonstrate decreased transmembrane TNFα (tmTNFα) expression, but not soluble (sTNFα) production by patient IFN-DCs, determines defective...
<h3>Objective:</h3> Glioma is a highly invasive tumor, frequently disposed in essential areas of the brain, which makes its surgical excision extremely difficult; meanwhile adjuvant therapy remains quite ineffective. <h3>Methods:</h3> In current report, new therapeutic approach curing malignant neoplasms has been performed on U87 human glioblastoma model. This approach, termed "Karanahan", aimed at eradication cancer stem cells (CSCs), were recently shown to be capable internalizing...
Besides an antigen-presenting function and ability to induce antitumor immune responses, dendritic cells (DCs) possess a direct tumoricidal activity. We previously reported that monocyte-derived IFNα-induced DCs (IFN-DCs) of glioblastoma multiforme patients express low levels membrane TNFα molecule (mTNFα) have impaired TNFα/TNF-R1-mediated cytotoxicity against immortalized tumor cell line HEp-2. However, whether the observed defect could affect killer activity glioma patient autologous...
In present work, a comparative analysis of cytostatic and cytotoxic activity IL-4 IFNα-induced dendritic cells (IL-4- IFN-DCs) has been performed in healthy donors patients with brain tumors. Cytostatic was evaluated, as capacity DCs to inhibit growth NK-resistant HEp-2 A-549 tumor cell lines, whereas measured by assessment lysis. As compared IL4-DC, IFN-DCs from displayed more pronounced effect similar against cells. the tumors, were characterized decreased activity. Meanwhile, malignant...
We report a clinical case of cerebral actinomycotic granuloma that was preoperatively diagnosed as an intracerebral tumor. On the basis histological examination performed after lesion resection, diagnosis made. The article presents long-term outcomes surgery. literature data on cases various forms actinomycosis are provided.Представлено клиническое наблюдение актиномикотической гранулемы головного мозга, которое на дооперационном этапе диагностировано как внутримозговая опухоль. После...
Primary cultures of human glioblastoma were obtained from the surgical material patients K. (female, 61 years, Ds: relapse glioblastoma) and Zh. 60 glioblastoma). The effectiveness a new therapeutic approach aimed at destroying cancer cell community was evaluated on primary lines culture by employing strategy tumor-initiating stem synchronization domestic their eradication "3+1". key elements following indicator results: (1) evaluation presence cells in population analyzed ability to...
Although major progress has been made in the standard treatment for glioblastomas, encompassing maximal surgical resection, chemotherapy and radiation therapy, it is possible to increase survival rates significantly only a few patients. Therefore, necessary explore new therapeutic modalities, one of which immunotherapy. The aim study was evaluate efficacy combined use autologous pooled tumor lysates comprehensive patients with glioblastoma.All (n=58, including 30 males 28 females aged 18-70...
The cosmetic outcome of cranioplasty performed with individual implants is an important component in assessing the results surgical reconstructions scull defects. Developing temporalis muscle atrophy affects harmonic face contour and, cases using patient-specific (PSI), designed taking into account bone symmetry, may discredit this technique due to unsatisfactory results. In regard, urgent task find possibility compensating for a visual defect by modifying shape order achieve good 54 were...
Techniques for surgical correction of Chiari malformation vary significantly. A number authors report about occipital bone reconstruction at the final stages. The article provides an overview cranioplasty techniques in interventions malformation.
The aim of the study was to evaluate efficacy a novel technique for preparation, staining, and visualization tissues containing extra-skeletal mineralization areas, all-metal implants or their prototypes subsequent examination using scanning electron microscopy in backscattered mode.After fixation 10% formalin (24 h), biomaterial (a titanium nickelide plate with surrounding after subcutaneous implantation, patented alloy plates cranioplasty, primary secondary calcified atherosclerotic...
Background. Despite its long history, cranioplasty remains a topical problem of neurosurgery, due to the increasing number traumatic brain injury, consequences which are main driver in preserving population patients with postcranioectomy skull defects. As rule, these defects extensive, and use individual implants cases is method choice. Over past 5 years, products created by medical additive manufacturing has increased, this regard, assessment long-term results surgical interventions their...