The mixture of phosphopeptides enriched from proteome samples are very complex. To reduce the complexity it is necessary to fractionate phosphopeptides. However, conventional enrichment methods typically only enrich but not In this study, application strong anion exchange (SAX) chromatography for and fractionation was presented. It found that were highly by SAX majority unmodified peptides did bind onto SAX. Compared with Fe(3+) immobilized metal affinity (Fe(3+)-IMAC), almost double...

Pores for effect: Silica particles with a pore size of 20.5 Å (see TEM image) are effective enriching peptides in human plasma over wide range molecular weights from 1 to 12 kDa, while repelling most other proteins outside. The structure the material makes it superior peptide enrichment compared adsorbent- and ultrafiltration-based methods.

Phosphorylation is one of the most important post-translational modifications proteins, which modulates a wide range biological functions and activity proteins. The analysis phosphopeptides still challenging tasks in proteomics research by mass spectrometry. In this study, novel phosphopeptide enrichment approach based on strong interaction zirconium phosphonate (ZrP) modified surface with has been developed. ZrP porous silicon (ZrP-pSi) wafer was prepared to specifically capture from...

Abstract Background Grain protein content (GPC) is an important quality determinant for barley used as malt, feed well food. It controlled by a complex genetic system. GPC differs greatly among genotypes and also variable across different environments. imperative to understand the control of identify with less variation under Results In this study, 59 cultivated 99 Tibetan wild were genome-wide association study (GWAS) multi-platform candidate gene-based analysis, in order molecular markers...

Exploring bone proteome is an important and challenging task for understanding the mechanisms of physiological/pathological process tissue. However, classical methods protein extraction soft tissues cells are not applicable Therefore, method development efficient critical analysis. We found in this study that efficiency was improved significantly when tissue demineralized by hydrochloric acid (HCl). A sequential developed large-scale analysis The first HCl solution then extracted using three...

Large-scale characterization of phosphoproteins requires highly specific methods for the purification phosphopeptides because low abundance and substoichiometry phosphorylation. A phosphopeptide enrichment method using ZrO2 nanoparticles is presented. The high specificity this approach was demonstrated by isolation from digests model phosphoproteins. strong affinity to enables a complex peptide mixture in which two orders magnitude lower than that nonphosphopeptides. Superior selectivity...

A 150 μm internal diameter capillary monolithic column with a strong cation-exchange stationary phase was prepared by direct in situ polymerization of ethylene glycol methacrylate phosphate and bisacrylamide trinary porogenic solvent consisting dimethylsulfoxide, dodecanol, N,N'-dimethylformamide. This exhibits higher dynamic binding capacity, faster kinetic adsorption peptides, more than 10 times permeability the packed commercially available particles. It applied as trap nanoflow liquid...

There are vast archives of formalin-fixed tissues spanning many conceivable conditions such as different diseases, time courses, and treatment allowing acquisition the necessary numbers samples to carry out biomarker discovery study. However, conventional protein analysis approach is not applicable for proteins in tissue because formalin fixation process resulted cross-linking proteins, thus, intact cannot be efficiently extracted. In this study, several protocols were investigated extract...

A nanoliter trypsin-based monolithic microreactor coupled with μRPLC−MS/MS was reported for shotgun proteome analysis. The proteins were rapidly digested by the microreactor, and resulting protein digests directly loaded onto a μRPLC column separation followed detection of eluted peptides tandem mass spectrometer. digestion efficiency stability demonstrated using bovine serum albumin as model protein. When compared an incubation time more than 10 h free trypsin in conventional approach,...

The immunomodulatory drug (IMiD) thalidomide and its structural analogs lenalidomide pomalidomide are highly effective in treating clinical indications. Thalidomide binds to cereblon (CRBN), a substrate receptor of the cullin-4 really interesting new gene (RING) E3 ligase complex. Here, we examine effect on CRBN ubiquitination functions human cell lines. We find that ubiquitin modification includes K48-linked polyubiquitin chains blocks formation CRBN-ubiquitin conjugates. Furthermore, show...

An improved strategy for the preparation of octadecylated silica monolith capillary column with high homogeneity was proposed. Column performance evaluated by nanoscale HPLC. The design constructing an integrated nanoelectrospray emitter on first introduced. In comparison separated configuration where is connected to monolithic aid a zero dead volume union, has inherent advantage minimized extracolumn thus providing separation quality. BSA tryptic digest, and peak capacity 313 30-cm...

Abstract Immobilized metal affinity chromatography (IMAC) is a commonly used technique for phosphoproteome analysis due to its high adsorption of phosphopeptides. Miniaturization IMAC column essential the small amount sample. Nanoscale was prepared by chemical modification silica monolith with iminodiacetic acid (IDA) followed immobilization Fe 3+ ion inside capillary. It demonstrated that ‐IDA monolithic capillary could specifically capture phosphopeptides from tryptic digest α‐casein...

Fe3+-immobilized mesoporous molecular sieves MCM-41 with particle size of ca. 600 nm and pore 3 is synthesized applied to selectively trap separate phosphopeptides from tryptic digest proteins. For the capture phosphopeptides, typically 10 μL solution was first diluted 1 mL by ACN/0.1% TFA (50:50, v/v) incubated 0.1% acetic acid dispersed for h under vibration. trapped separated centrifugation. The deposition washed a volume 300 containing 100 mM NaCl in followed remove nonspecifically bound...

The transmission spectrum of apples is affected by the fruit's size, which leads to poor prediction performance soluble solids content (SSC) models built for their different apple sizes. In this paper, three sets near infrared (NIR) spectra with various diameters were collected applying NIR spectroscopy detection equipment compare differences among diameter groups. corrected studying extinction rates within apples. used develop a partial least squares model content. Compared without size...

Da-Yuan-Yin is a Chinese traditional prescription.This study explores the therapeutic effects of decoction polyphenol fraction (DYY-4) on acute lung injury (ALI) in mice induced by lipopolysaccharide (LPS).The (n = 10) were orally administrated with DYY-4 (15, 30, and 60 mg/kg) or DXM (5 mg/kg), half an hour after LPS (2 instilled intratracheally. The protein content levels inflammatory factors, complements, mRNA expression inducible nitric oxide synthase (iNOS) cyclooxygenase-2 (COX-2),...

This study aims to comprehensively evaluate the internal quality changes in apples during storage via near-infrared spectroscopy. Specifically, we focus on performance differences different apple varieties under diverse conditions and construct predictive models determine optimal period. By using spectroscopy technology, 384 samples of four (Xinjiang Akesu, Wafangdian Huangyuanshuai, Shandong Fuji, Luochuan Fuji) were analyzed monitor their soluble solid content (SSC) fruit firmness within 7...