A5100353919- Glycosylation and Glycoproteins Research
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Galectins and Cancer Biology
- NF-κB Signaling Pathways
- Peptidase Inhibition and Analysis
- Carbohydrate Chemistry and Synthesis
- Monoclonal and Polyclonal Antibodies Research
- Proteoglycans and glycosaminoglycans research
- Metabolomics and Mass Spectrometry Studies
- Analytical chemistry methods development
- Advanced biosensing and bioanalysis techniques
- Chromatography in Natural Products
- Catalytic Processes in Materials Science
- Lysosomal Storage Disorders Research
- Infant Nutrition and Health
- Respiratory viral infections research
- vaccines and immunoinformatics approaches
- Supramolecular Self-Assembly in Materials
- Synthesis and bioactivity of alkaloids
- Zeolite Catalysis and Synthesis
- Electrostatics and Colloid Interactions
- Infectious Diseases and Mycology
- Advanced NMR Techniques and Applications
Changchun University of Science and Technology
2025
Sphere Medical (United Kingdom)
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
Chungnam National University
2020-2022
Inner Mongolia University
2022
Yueyang Hospital
2021
Shanghai University of Traditional Chinese Medicine
2021
Peking University
2021
Peking University Third Hospital
2021
Dalian Institute of Chemical Physics
2002-2019
Phosphorylation is one of the most important post-translational modifications proteins, which modulates a wide range biological functions and activity proteins. The analysis phosphopeptides still challenging tasks in proteomics research by mass spectrometry. In this study, novel phosphopeptide enrichment approach based on strong interaction zirconium phosphonate (ZrP) modified surface with has been developed. ZrP porous silicon (ZrP-pSi) wafer was prepared to specifically capture from...
Large-scale characterization of phosphoproteins requires highly specific methods for the purification phosphopeptides because low abundance and substoichiometry phosphorylation. A phosphopeptide enrichment method using ZrO2 nanoparticles is presented. The high specificity this approach was demonstrated by isolation from digests model phosphoproteins. strong affinity to enables a complex peptide mixture in which two orders magnitude lower than that nonphosphopeptides. Superior selectivity...
In this work, a highly efficient profiling of N-linked glycans was achieved by facile and eco-friendly synthesized porous metal-free carbon material. The derived from well-defined nanorod zinc metal–organic framework via the metal removal under high-temperature carbonization, which exhibited specific surface area 1700 m2/g. After further oxidation, oxidized applied to selective isolation complex biological samples due strong interaction between glycan as well size-exclusion mechanism. Twenty...
Peptidome analysis has received increasing attention in recent years. Cancer diagnosis by serum peptidome also been reported peptides' profiling for discovery of peptide biomarkers. Tissue, which may have a higher biomarker concentration than blood, not investigated extensively means analysis. Here, method the mouse liver was developed combination size exclusion chromatography (SEC) prefractionation with nano-liquid chromatography−tamdem mass spectrometry (nanoLC−MS/MS) The extracted...
Graphene nanopore has been promising the ultra-high resolution for DNA sequencing due to atomic thickness and excellent electronic properties of graphene monolayer. The dynamical translocation phenomena and/or behaviors underneath blocked ionic current, however, have not well unveiled date electrophoretically through a nanopore. In this report, assessment on sensitivity current instantaneous statuses in 2.4 nm was carried out based all-atom molecular dynamics simulations. By filtering...
Abstract A mode of comprehensive 2‐D LC was developed by coupling a silica‐bonded HSA column to silica monolithic ODS column. This system combined the affinity property and high‐speed separation ability The chromatography with HSA‐immobilized stationary phase applied study interaction multiple components in traditional Chinese medicines (TCMs) according their protein first dimension. Then unresolved retained on were further separated second By hyphenating diode array detector MS detectors,...
Poren zeigen Wirkung: Mit Siliciumoxidpartikeln mit einer Porengröße von 20.5 Å (siehe TEM-Bild) gelingt die Anreicherung Peptiden Molekulargewichten 1 bis 12 kDa in Humanplasma; andere Plasmaproteine werden dagegen nicht angereichert. Durch seine Porenstruktur ist das Material besser für Peptidanreicherung geeignet als Adsorbens- und Ultrafiltrationsmethoden.
Human plasma contains a complex matrix of proteolytically derived peptides (plasma peptidome) that may provide correlate biological events occurring in the entire organism. Analyzing these from small amount serum/ is difficult due to complexity sample and low levels peptides. Here, we describe novel peptidome analysis approach using multiwalled carbon nanotubes (MWCNTs) as an alternative adsorbent capture endogenous human plasma. Harvested were analyzed by liquid chromatography-mass...
Abstract Three nitrophenol isomer‐imprinted polymers were prepared under the same conditions using 4‐vinylpyridine as a functional monomer. Different recognition capacities for template molecules observed three polymers. Another imprinting system with stronger acidity than isomers, 2‐hydroxybenzoic acid (salicylic acid) and 4‐hydroxybenzoic acid, was imprinted or acrylamide monomer respectively. Both acid‐imprinted two monomers showed ability molecule. However, when chosen monomer, salicylic...
Glycosylation is a valuable tool for modulating protein solubility; however, the lack of reliable research strategies has impeded efficient progress in understanding and applying this modification. This study aimed to bridge gap by investigating solubility model glycoprotein molecule, carbohydrate-binding module (CBM), through two-stage process. In first stage, an approach involving chemical synthesis, comparative analysis, molecular dynamics simulations library glycoforms was employed...
Using molecular dynamics simulations, we investigated an integrated bio-nano interface consisting of a β-sheet protein stacked onto graphene. We found that the stacking assembly model on graphene could be controlled by water molecules. The interlayer filled within interstices suppress vibration surface groups and impair CH···π interaction driving attraction intermolecular coupling would relaxed relative motion upon due to between surface. This effect reduced hindrance against graphene,...
Abstract Background Glycosylation is an important post‐translational modification of protein. The change in glycosylation involved the occurrence and development various diseases, this study verified that N ‐glycan markers might be a diagnostic marker psoriasis. Methods A total 76 psoriasis patients were recruited. We used Psoriasis Area Severity Index (PASI) scores to evaluate state psoriasis, 41 whom divided into three subgroups: mild, moderate, severe. At same time, healthy subjects...
In this work, an enrichment approach for the profiling of N-linked glycans was developed by utilizing a highly porous 3D graphene composite fabricated from oxide nanosheets and phenol-formaldehyde polymer via graphitization KOH activation. tailoring large surface area (ca. 2213 m2 g-1) 3D-layered mesoporous structure, demonstrated not only high efficiency in glycan but also size-exclusion effect against residual protein interference. For standard ovalbumin digest, 26 were identified with...
Other SectionsAbstractINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTSCONFLICT OF INTERESTReferences
In this study, diclofenac, a non-steroidal anti-inflammatory drug, was investigated for its potential effect on the gene expression and production of airway MUC5AC mucin. The human respiratory epithelial NCI-H292 cells were pretreated with diclofenac 30 min stimulated phorbol 12-myristate 13-acetate (PMA), following 24 h. PMA-induced nuclear factor kappa B (NF-kB) signaling pathway also investigated. Diclofenac suppressed mucins, induced by PMA through inhibition degradation inhibitory Bα...
Other SectionsAbstractINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONACKNOWLEDGMENTSCONFLICT OF INTERESTReferences