A5015338372- Pluripotent Stem Cells Research
- Muscle Physiology and Disorders
- Acute Myeloid Leukemia Research
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Retinoids in leukemia and cellular processes
- Chronic Lymphocytic Leukemia Research
- Hematopoietic Stem Cell Transplantation
- Chronic Myeloid Leukemia Treatments
- Neurogenetic and Muscular Disorders Research
- Amyotrophic Lateral Sclerosis Research
University of Wisconsin–Madison
2013-2024
Overactive RAS signaling is prevalent in juvenile myelomonocytic leukemia (JMML) and the myeloproliferative variant of chronic (MP-CMML) humans, both are refractory to conventional chemotherapy. Conditional activation a constitutively active oncogenic Nras (NrasG12D/G12D) murine hematopoietic cells promotes an acute neoplasm (MPN) that recapitulates many features JMML MP-CMML. We found NrasG12D/G12D-expressing HSCs, which serve as JMML/MP-CMML–initiating cells, show strong hyperactivation...
Human myogenic progenitors can be derived from pluripotent stem cells (PSCs) for use in modeling natural and pathological myogenesis, as well treating muscle diseases. Transgene-free methods of deriving different PSC lines often produce mixed populations that are heterogeneous differentiation potential, yet detailed accurate characterization human PSC-derived remains elusive the field. The isolation purification is thus an important methodological consideration when we investigate properties...
Introduction The changing composition of non-cell autonomous circulating factors in blood as humans age is believed to play a role muscle mass and strength loss. mechanisms through which these act age-related skeletal changes not fully understood. In this study, we used human myogenic progenitors derived from pluripotent stem cells study roles during the process differentiation. Methods Myogenic embryonic (ESCs) induced (iPSCs) were supplemented with serum samples aged or young Fischer 344 ×...
Abstract Overactive Ras signaling is prevalent in human juvenile and chronic myelomonocytic leukemias (JMML/CMML) that are refractory to conventional chemotherapy. Conditional activation of endogenous NrasG12D/G12D hematopoietic cells mice leads an acute myeloproliferative disease (MPN) recapitulates many features JMML variant CMML. We found as JMML/CMML initiating cells, stem (HSCs) show strong ERK1/2 hyperactivation, hyperproliferation, depletion with concomitant expansion downstream...