A5010337152- Glioma Diagnosis and Treatment
- 3D Printing in Biomedical Research
- Meningioma and schwannoma management
- Barrier Structure and Function Studies
- Neurofibromatosis and Schwannoma Cases
- Brain Metastases and Treatment
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Nanoparticle-Based Drug Delivery
- CAR-T cell therapy research
- Cancer Research and Treatments
- Microtubule and mitosis dynamics
- Enzyme function and inhibition
- Cancer Mechanisms and Therapy
- Virus-based gene therapy research
- Protease and Inhibitor Mechanisms
- Radiomics and Machine Learning in Medical Imaging
- Cancer Genomics and Diagnostics
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Melanoma and MAPK Pathways
- Cancer-related cognitive impairment studies
- Ear and Head Tumors
- Cell Adhesion Molecules Research
- Protein Kinase Regulation and GTPase Signaling
Universitätsklinikum Würzburg
2011-2025
University of Würzburg
2012-2023
Klinik und Poliklinik für Neurologie
2022-2023
Biologie Labor
2020
Max Delbrück Center
2013-2017
University Medical Center
2017
Harvard University
2013
The Netherlands Cancer Institute
2013
Dana-Farber Cancer Institute
2013
Umeå University
2013
Trophoblast cell (CTB) invasion into the maternal endometrium plays a crucial role during human embryo implantation and placentation. As for all invasive types, ability of CTB to infiltrate uterine wall is facilitated by activity matrix metalloproteinases (MMPs), which regulated tissue inhibitors MMPs (TIMPs). There evidence expression several TIMPs in decidua. However, published data are limited. Therefore, set foundation future research, we screened panel healthy deciduas obtained first,...
Glioblastomas are characterized by an aggressive local growth pattern, a marked degree of invasiveness and poor prognosis. Tumor is facilitated the increased activity proteolytic enzymes which involved in destruction extracellular matrix surrounding healthy brain tissue. Elevated levels metalloproteinases (MMPs) were found glioblastoma (GBM) cell-lines, as well GBM biopsies compared with low-grade astrocytoma (LGA) normal samples, indicating role malignant progression. A careful review...
BackgroundGlioblastomas exhibit a high level of chemotherapeutic resistance, including to the antimitotic agents vincristine and taxol. During mitotic agent-induced arrest, glioblastoma cells are able perform damage-control self-repair continue proliferation. Monopolar spindle 1 (MPS1/TTK) is checkpoint kinase gatekeeper arrest.
Despite the availability of numerous therapeutic substances that could potentially target CNS disorders, an inability these agents to cross restrictive blood–brain barrier (BBB) limits their clinical utility. Novel strategies overcome BBB are therefore needed improve drug delivery. We report, for first time, how Tumor Treating Fields (TTFields), approved glioblastoma (GBM), affect BBB’s integrity and permeability. Here, we treated murine microvascular cerebellar endothelial cells (cerebEND)...
In a recent study, we showed in an vitro murine cerebellar microvascular endothelial cell (cerebEND) model as well vivo rats that Tumor-Treating Fields (TTFields) reversibly open the blood-brain barrier (BBB). This process is facilitated by delocalizing tight junction proteins such claudin-5 from membrane to cytoplasm. investigating possibility same effects could be observed human-derived cells, 3D co-culture of BBB was established consisting primary brain cells (HBMVEC) and immortalized...
Tumor treating fields (TTFields) are approved for glioblastoma (GBM) therapy. TTFields disrupt cell division by inhibiting spindle fiber formation. Spindle assembly checkpoint (SAC) inhibition combined with antimitotic drugs synergistically decreases glioma growth in culture and mice. We hypothesized that SAC will increase efficacy. Human GBM cells (U-87 MG, GaMG) were treated (200 kHz, 1.7 V/cm) and/or the inhibitor MPS1-IN-3 (IN-3, 4 µM). Cells counted after 24, 48, 72 h of treatment at 24...
Abstract Background The most threatening metastases in breast cancer are brain metastases, which correlate with a very poor overall survival, but also limited quality of life. A key event for the metastatic progression into is migration cells across blood–brain barrier (BBB). Methods We adapted and validated CD34 + cells-derived human vitro BBB model (brain-like endothelial cells, BLECs) to analyse effects patient serum on properties. collected samples from healthy donors, patients primary...
Background. While chimeric antigen receptor (CAR) T cells have achieved encouraging remission rates in hematological malignancies, they demonstrated limited success treating Glioblastoma (GBM), particularly due to high intra- and intertumoral heterogeneity. In this study, we identified a relevant preserved target antigen, Podoplanin (PDPN), evaluated the potential of PDPN- GD2-CAR cell blend overcome GBM Methods. Target screening included clinical samples, healthy tissues lines, as well...
Two protein kinases that are involved in proliferation and oncogenesis but so far were thought to be functionally independent Raf CK2. The signaling pathway is known play a critical role such fundamental biological processes as cellular differentiation. Abnormal activation of this potentially oncogenic. Protein kinase CK2 exhibits enhanced levels solid human tumors proliferating tissue. In two‐hybrid screen mouse‐embryo cDNA library we detected an interaction between A‐Raf CK2β subunit. This...
Metastasis-associated in colon cancer 1 (MACC1) has been established as an independent prognostic indicator of metastasis formation and metastasis-free survival for patients with other solid tumors. However, no data are available concerning MACC1 expression human astrocytic Glioblastoma multiforme (GBM) is the most prevalent primary brain tumor adulthood, due to its invasive rapid growth, have unfavorable prognoses. Although these tumors rarely metastasize, their migratory behavior similar...
Progressive deterioration of the central nervous system (CNS) is commonly associated with aging. An important component neurovasculature blood-brain barrier (BBB), majorly made up endothelial cells joined together by intercellular junctions. The relationship between senescence and changes in BBB has not yet been thoroughly explored. Moreover, lack vitro models for study mechanisms involved those impede further more in-depth investigations field. For this reason, we herein present an model...
Gene expression studies related to cancer diagnosis and treatment are becoming more important. Housekeeping genes that absolutely reliable essential for these normalize gene expression. An incorrect choice of housekeeping leads interpretation errors experimental results including evaluation quantification pathological Here, we examined (a) the degree regulation GAPDH in human glioblastoma cells under hypoxic conditions vitro comparison other like β-actin, serving as loading controls, (b)...
// Maria Breun 1 , Alexandra Schwerdtfeger Donato Daniel Martellotta Almuth F. Kessler Jose M. Perez Camelia M Monoranu 2, 3 Ralf-Ingo Ernestus Cordula Matthies Mario Löhr 1, * and Carsten Hagemann Department of Neurosurgery, University Hospital Würzburg, 97080 Germany 2 Neuropathology, Institute Pathology, Comprehensive Cancer Center (CCC), Mainfranken, Würzburg These authors contributed equally to this work Correspondence to: Breun, email: Breun_M@ukw.de Keywords: vestibular schwannoma;...
Gene expression studies related to cancer diagnosis and treatment are important. In order conduct such experiment accurately, absolutely reliable housekeeping genes essential normalize gene expression. The most important characteristics of their presence in all cells levels remain relatively constant under different experimental conditions. However, no single this group manifests always stable Incorrect choice leads interpretation errors results including evaluation quantification...
Two square-planar palladium(II) and platinum(II) azido complexes [M(N3)(L)] with L = N-phenyl-2-[1-(2-pyridinyl)ethylidene]hydrazine carbothioamide reacted four different electron-poor alkynes R-C≡C-R' R R' COOCH3, COOEt, COOCH2CH2OCH3 or CF3, COOEt in a [3 + 2] cycloaddition "iClick" reaction. The resulting triazolate [M(triazolateR,R')(L)] were isolated by simple precipitation and/or washing high purity good yield. Six out of the eight new compounds feature ligand coordinated to metal...
Despite its significant overexpression in several malignant neoplasms, the expression of RPS27 central nervous system (CNS) is widely unknown. We identified cell types expressing CNS under normal and disease conditions. acquired specimens healthy brain (NB), adult pilocytic astrocytoma (PA) World Health Organization (WHO) grade I, anaplastic PA WHO III, gliomas II/III with or without isocitrate dehydrogenase (IDH) mutation, glioblastoma multiforme (GBM). protein was examined by...