A5031620334- Surfactants and Colloidal Systems
- Polymer crystallization and properties
- Spectroscopy and Quantum Chemical Studies
- Barrier Structure and Function Studies
- Connexins and lens biology
- Lipid Membrane Structure and Behavior
- Thermodynamic properties of mixtures
- Hepatitis B Virus Studies
- Bacteriophages and microbial interactions
- Pancreatic function and diabetes
- Hepatitis C virus research
- Textile materials and evaluations
- High-Velocity Impact and Material Behavior
- Electrostatics and Colloid Interactions
- Nanopore and Nanochannel Transport Studies
- Retinal Diseases and Treatments
- Nuclear Physics and Applications
- Force Microscopy Techniques and Applications
- Gut microbiota and health
- Molecular Junctions and Nanostructures
- Neurological Disease Mechanisms and Treatments
- Reproductive System and Pregnancy
- Fuel Cells and Related Materials
- Glaucoma and retinal disorders
- Fiber-reinforced polymer composites
Kansai University
2010-2024
Nagoya University
2014-2023
Fujimoto Dental and Oral Surgery Clinic
2017-2023
John Wiley & Sons (United States)
2021
University of Occupational and Environmental Health Japan
2020
Chiba University
2017-2018
Tokyo University of Science
2018
Ritsumeikan University
2014
Fukui Prefectural University
1999-2007
National Institute of Advanced Industrial Science and Technology
2006
Occludin is the only known integral membrane protein localizing at tight junctions (TJ), but recent targeted disruption analysis of occludin gene indicated existence as yet unidentified proteins in TJ. We therefore re-examined isolated junction fraction from chicken liver, which was first identified. Among numerous components this fraction, a broad silver-stained band ∼22 kD detected with through 4 M guanidine-HCl extraction well sonication followed by stepwise sucrose density gradient...
Two related integral membrane proteins, claudin-1 and -2, recently were identified as novel components of tight junction (TJ) strands. Here, we report six more claudin-like indicating the existence a claudin gene family. Three these reported previously RVP1, Clostridium perfringens enterotoxin receptor, TMVCF, but their physiological functions not determined. Through similarity searches followed by PCR, isolated full length cDNAs mouse TMVCF well three proteins designated them claudin-3 to...
Three integral membrane proteins, clau- din-1, -2, and occludin, are known to be components of tight junction (TJ) strands. To examine their ability form TJ strands, cDNAs were introduced into mouse L fibroblasts lacking TJs. Immunofluorescence microscopy revealed that both FLAG-tagged claudin-1 -2 highly concentrated at cell contact sites as planes through a homophilic interaction. In freeze-fracture replicas these sites, well-developed networks strands identified similar strand in situ...
ABSTRACT Endothelial cells provide a crucial interface between blood and tissue environments. Free diffusion of substances across endothelia is prevented by the endothelial tight junction, permeability which varies enormously depending on tissue. blood-brain barrier possess junctions severely limited permeability, whereas those non-neural are considerably leakier, but molecular basis for this difference not clear. Occludin major transmembrane protein localizing at junction. In study, we...
Occludin is the only known integral membrane protein of tight junctions (TJs), and now believed to be directly involved in barrier fence functions TJs. Occludin-deficient embryonic stem (ES) cells were generated by targeted disruption both alleles occludin gene. When these subjected suspension culture, they aggregated form simple, then cystic embryoid bodies (EBs) with same time course as EB formation from wild-type ES cells. Immunofluorescence microscopy ultrathin section electron revealed...
Members of the newly identified claudin gene family constitute tight junction (TJ) strands, which play a pivotal role in compartmentalization multicellular organisms. We oligodendrocyte-specific protein (OSP) as claudin-11, new member, due to its sequence similarity claudins well ability form TJ strands transfected fibroblasts. Claudin-11/OSP mRNA was expressed brain and testis. Immunofluorescence microscopy with anti–claudin-11/OSP polyclonal antibody (pAb) anti-neurofilament mAb revealed...
We propose a new electron microscopic method, the sodium dodecylsulphate (SDS)-digested freeze-fracture replica labeling technique, to study two-dimensional distribution of integral membrane proteins in cellular membranes. Unfixed tissue slices were frozen with liquid helium, freeze-fractured, and replicated platinum/carbon evaporator. They digested 2.5% SDS solubilize unfractured membranes cytoplasm. While detergent dissolved cytoplasm, it did not extract fractured halves. After...
ABSTRACT Occludin is an integral membrane protein localizing at tight junctions with four transmembrane domains. When chicken occludin was overexpressed in insect cells by recombinant baculovirus infection, peculiar multilamellar structures accumulated the cytoplasm. Partial isolation of these indicated that introduced highly enriched structures. Thin section electron microscopy revealed each lamella transformed from intracellular membranous cisternae whose luminal space completely...
The number of AMPA receptor (AMPAR) is the major determinant synaptic strength at glutamatergic synapses, but little known about absolute and density AMPARs in individual synapses. Using SDS-digested freeze-fracture replica labeling, which has high detection efficiency comparable with electrophysiological noise analysis for functional AMPAR, we analyzed three kinds excitatory synapses molecular layer adult rat cerebellum. In parallel fiber (PF)–Purkinje cell (PC) found large variability...
The hierarchical self-assemblies of hydrophobically modified pullulan, such as cholesteryl-bearing pullulan (CHP) and hexadecyl group-bearing (C16P), were investigated. In a dilute aqueous solution, they formed hydrogel nanoparticles. semidilute solution above approximately 2% (w/w), the viscosity CHP or C16P drastically increased. At higher concentration, macroscopic gels: gave structure in which nanoparticles are linked, while macrogel has mainly fibrous network structure. rheological...
Occludin is the only integral membrane protein identified to date as a component of tight junctions (TJs). Here, we examined distribution and expression occludin in murine testis bearing well-developed TJ. In adult mouse testis, was concentrated at TJ strands, which are located most basal regions lateral membranes Sertoli cells. immunoblotting, showed characteristic multiple banding pattern, suggesting that highly phosphorylated testis. 1-wk-old distributed diffusely cells, even this stage,...
Our new molecular dynamics (MD) simulation program, MODYLAS, is a general-purpose program appropriate for very large physical, chemical, and biological systems. It equipped with most standard MD techniques. Long-range forces are evaluated rigorously by the fast multipole method (FMM) without using Fourier transform (FFT). Several methods have also been developed extremely fine-grained parallelism of calculation. The virtually buffering-free communications arithmetic operations, minimal...
Small viruses that belong, for example, to the Picornaviridae, such as poliovirus and foot-and-mouth disease virus, consist simply of capsid proteins a single-stranded RNA (ssRNA) genome. The capsids are quite stable in solution protect genome from environment. Here, based on long-time large-scale 6.5 × 106 all-atom molecular dynamics calculations Mahoney strain poliovirus, we show microscopic properties viral at level. First, found equilibrium rapid exchange water molecules across capsid....
In this study, we investigated the molecular dynamics of polyrotaxane (PR), composed α-cyclodextrins (CDs) and a poly(ethylene glycol) (PEG) axial chain, in solution by means quasi-elastic neutron scattering (QENS) measurements full-atomistic (MD) simulations. From QENS experiments, estimated diffusion coefficients CD PEG monomers PR, which are quantitative agreement with those obtained MD By analyzing simulation results, succeeded, for first time, observing quantifying sliding motion along...
Cinnamycin is a unique toxin in that its receptor, phosphatidylethanolamine (PE), resides the inner layer of plasma membrane. Little known about how recognizes PE and causes cytotoxicity. We showed cinnamycin induced transbilayer phospholipid movement target cells leads to exposure leaflet toxin. Model membrane studies revealed lipid concentration-dependent manner. Re-orientation phospholipids was accompanied by an increase incidence β-sheet structure cinnamycin. When surface concentration...
The authors propose a novel method to evaluate the position-dependent diffusion constant by analyzing unperturbed segments of trajectory determined additional flat-bottom potential. accuracy this is first established studying homogeneous systems, where reference value can be obtained Einstein relation. applicability new heterogeneous systems then demonstrated hydrophobic solute near wall. proposed also comprehensively compared with popular conventional methods, whereby significance present...
Journal Article Immunohistochemical localization of superoxide dismutase in the human ovary Get access Masahide Shiotani, Shiotani 1 1To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Yoichi Noda, Noda Department Gynecology and Obstetrics, Kyoto UniversitySakyo-ku, 606, Japan Katsuhiko Narimoto, Narimoto Kimitoshi Imai, Imai Mori Takahide, Takahide Kazushi Fujimoto, Fujimoto 2Department Anatomy, Faculty Medicine, Kazuo...
The origin of high solubility poly(oxyethylene) in water has been an open question. Although it is thought that the arises from increase trans-gauche-trans (tgt) conformer water, relationship between tgt and unclear. In this study, we have investigated conformational equilibria 1,2-dimethoxyethane, which a model molecule for poly(oxyethylene), by using Raman spectroscopy, determined change population free energy each with aid density functional theory calculations. transfer pure liquid to...