A5031220657- Cardiomyopathy and Myosin Studies
- Single-cell and spatial transcriptomics
- Birth, Development, and Health
- Mitochondrial Function and Pathology
- Chemotherapy-induced cardiotoxicity and mitigation
- CRISPR and Genetic Engineering
- Selenium in Biological Systems
- PARP inhibition in cancer therapy
- Advanced Breast Cancer Therapies
- IL-33, ST2, and ILC Pathways
- RNA Research and Splicing
- Cardiac electrophysiology and arrhythmias
- Pluripotent Stem Cells Research
- Molecular Biology Techniques and Applications
- Whipple's Disease and Interleukins
- Advanced Proteomics Techniques and Applications
- Galectins and Cancer Biology
- Polymer Nanocomposite Synthesis and Irradiation
- Congenital heart defects research
University Medical Center Groningen
2021-2025
University of Groningen
2021-2023
Harvard University
2019
Massachusetts General Hospital
2019
Phospholamban (PLN) p.Arg14del (R14del, R14∆/+) is the most commonly identified pathogenic variant that causes cardiomyopathy in Netherlands. Many disease characteristics are still unclear, including phenotypic triggers, progression and disease-specific biomarkers. We aim to gain a better understanding of R14∆/+ pathophysiology by establishing cohort across spectrum. The Disease spECifIc PatHways modifiERs PhosphoLambaN r14del (DECIPHER-PLN) includes 101 participants, categorised as...
Doxorubicin is an essential cancer treatment, but its usefulness hampered by the occurrence of cardiotoxicity. Nevertheless, pathophysiology underlying doxorubicin-induced cardiotoxicity and respective molecular mechanisms are poorly understood. Recent studies have suggested involvement cellular senescence.The aims this study were to establish whether senescence present in patients with investigate if could be used as a potential treatment target.Biopsies from left ventricles severe compared...
Abstract Aims Heart failure (HF) is recognized as an inflammatory disease in which cytokines play important role. In animal HF models, interleukin‐17A (IL‐17) has been linked to deterioration of cardiac function and fibrosis, whereas knock‐out IL‐17 showed beneficial effects. However, there limited evidence involvement patients with HF. This study aims investigate the clinical characteristics, outcomes, pathophysiological processes associated circulating concentrations Methods results was...
Members of the fetal-gene-program may act as regulatory components to impede deleterious events occurring with cardiac remodeling, and constitute potential novel therapeutic heart failure (HF) targets. Mitochondrial energy derangements occur both during early fetal development in patients HF. Here we aim elucidate role DIO2, a member fetal-gene-program, pluripotent stem cell (PSC)-derived human cardiomyocytes on mitochondrial dynamics energetics, specifically. RNA sequencing pathway...
A fundamental goal in the biological sciences is to determine how individual cells with varied gene expression profiles and diverse functional characteristics contribute development, physiology, disease. Here, we report a novel strategy assess cell physiology single living cells. Our approach utilizes fluorescently labeled mRNA-specific anti-sense RNA probes dsRNA-binding protein identify of specific genes real-time at single-cell resolution via FRET. We use this technology distinct...
Abstract Funding Acknowledgements Type of funding sources: Public grant(s) – EU funding. Main source(s): European Research Council CDK4/6 inhibitor Ribociclib induces cardiotoxicity in dynamic engineered heart tissues, mitigated by E2F1 overexpression Background Ribociclib, a novel chemotherapeutic agent for metastatic breast cancer, functions as cyclin-dependent kinase 4/6 (CDK4/6) inhibitor1. Its mechanism action involves the CDK4/6-Rb-E2F1 pathway. use has very recently been associated...
Abstract Background/Purpose: Phospholamban (PLN) p.Arg14del (R14del, R14Δ/+) is a pathogenic variant that can cause cardiomyopathy, characterized by PLN protein aggregation, ultimately leading to heart failure (HF). The exact pathophysiology unknown, we aim uncover R14Δ/+ disease mechanisms and study potential treatment using antisense oligonucleotides (PLN-ASOs). Methods Phosphoproteomics was performed on human tissue from end-stage patients (N=6) versus other etiologies of HF (N=10) as...
Objectives: To elucidate the role of DIO2, member fetal-gene-program, in PSC-derived human cardiomyocytes and on mitochondrial dynamics energetics, specifically. Background: Members fetal-gene-program may act as regulatory components to impede deleterious events that occur with cardiac remodeling constitute potential novel therapeutic heart failure (HF) targets. Mitochondrial energy metabolic derangements both during early fetal development patients HF. In HF, roadblocks structural damage...