A5015195827- Ferroptosis and cancer prognosis
- Heme Oxygenase-1 and Carbon Monoxide
- Inflammasome and immune disorders
- RNA modifications and cancer
- Erythrocyte Function and Pathophysiology
- Zebrafish Biomedical Research Applications
- interferon and immune responses
- Trace Elements in Health
- Analytical Chemistry and Sensors
- Protein Kinase Regulation and GTPase Signaling
- Gout, Hyperuricemia, Uric Acid
- Hormonal Regulation and Hypertension
- 3D Printing in Biomedical Research
- Drug Transport and Resistance Mechanisms
- Gas Sensing Nanomaterials and Sensors
- Hemoglobinopathies and Related Disorders
- Renal and related cancers
- Cell death mechanisms and regulation
- Space Exploration and Technology
- Cancer-related molecular mechanisms research
- Cancer, Lipids, and Metabolism
- Sphingolipid Metabolism and Signaling
- Immune cells in cancer
- Pluripotent Stem Cells Research
- GDF15 and Related Biomarkers
University Hospital Carl Gustav Carus
2019-2024
TU Dresden
2013-2024
University Medical Center Groningen
2024
Friedrich Schiller University Jena
1999-2017
Klinik und Poliklinik für Psychotherapie und Psychosomatik
2013
Abstract Acute kidney injury (AKI) is morphologically characterized by a synchronized plasma membrane rupture of cells in specific section nephron, referred to as acute tubular necrosis (ATN). Whereas the involvement necroptosis well characterized, genetic evidence supporting contribution ferroptosis lacking. Here, we demonstrate that loss suppressor protein 1 ( Fsp1 ) or targeted manipulation active center selenoprotein glutathione peroxidase 4 Gpx4 cys/- sensitize kidneys ferroptosis,...
Significance The currently used treatment regimen for the cancers of adrenal cortex involves mitotane, a nonspecific derivative pesticide DDT (1,1-(dichlorobiphenyl)-2,2-dichloroethane), which has an unclear mechanism action. Our studies in cell culture and genetic analysis public databases demonstrate that human adrenocortical carcinomas (ACCs) are remarkably sensitive to recently defined death pathway referred as ferroptosis, indicating induction ferroptosis could be promising approach...
Interleukin-4-induced-1 (IL4i1) is an amino acid oxidase secreted from immune cells. Recent observations have suggested that IL4i1 pro-tumorigenic via unknown mechanisms. As has homologs in snake venoms (L-amino oxidases [LAAO]), we used comparative approaches to gain insight into the mechanistic basis of how conserved regulate cell fate and function. Using mammalian expressed recombinant proteins, found venom LAAO kills cells hydrogen peroxide generation. By contrast, non-cytotoxic instead...
Dexamethasone is widely used as an immunosuppressive therapy and recently COVID-19 treatment. Here, we demonstrate that dexamethasone sensitizes to ferroptosis, a form of iron-catalyzed necrosis, previously suggested contribute diseases such acute kidney injury, myocardial infarction, stroke, all which are triggered by glutathione (GSH) depletion. GSH levels were significantly decreased dexamethasone. Mechanistically, identified up-regulated the metabolism regulating protein dipeptidase-1...
Abstract Signaling pathways of regulated necrosis, such as necroptosis and ferroptosis, contribute to acute kidney injury (AKI), but the role pyroptosis is unclear. Pyroptosis mediated by pore-forming protein gasdermin D (GSDMD). Here, we report a specific pattern GSDMD-protein expression in peritubular compartment mice that underwent bilateral ischemia reperfusion (IRI). Along similar lines, whole lysates increased during first 84 h following cisplatin-induced AKI. Importantly, unlike...
Small interfering RNAs (siRNAs) are widely used in biomedical research and clinical trials. Here, we demonstrate that siRNA treatment is commonly associated with significant sensitization to ferroptosis, independently of the target protein knockdown. Genetically targeting mitochondrial antiviral-signaling (MAVS) reversed siRNA-mediated sensitizing effect, but no activation canonical MAVS signaling, which involves phosphorylation IkBα interferon regulatory transcription factor 3 (IRF3), was...
The correct functioning of Ras proteins requires post‐translational modification the GTP hydrolases (GTPases). These modifications provide hydrophobic moieties that lead to attachment inner side plasma membrane. In this study we investigated role processing in interaction with various putative Ras‐effector proteins. We describe a specific, GTP‐independent between post‐translationally modified Ha‐ and Ki‐Ras4B G‐protein responsive phosphoinositide 3‐kinase p110γ. Our data demonstrate...
Abstract The cytosolic protein rubicon (RUBCN) has been implicated in the removal of necrotic debris and autoimmunity. However, role RUBCN models acute kidney injury (AKI), a condition that typically involves tubules, was not investigated. Here, we demonstrate RUBCN-deficient mice are hypersensitive to renal damage induced by ischemia-reperfusion (IRI) cisplatin-induced AKI. Combined deficiency mixed lineage kinase domain-like (MLKL) partially reversed sensitivity IRI model suggesting...
To introduce embryoid bodies derived from mouse embryonic stem (ES) cells, which differentiate blood vessel-like structures and leukocytes, as a novel in vitro model system for biocompatibility, inflammation, angiogenesis studies.Punched spherical discs of bioabsorbable polymers (ε-caprolactone L-lactide different compositions) with diameter 2 mm thickness 0.2 were inoculated cocultivation. As reference material biocompatible, nonbioabsorbable, bioincompatible materials, polymer punched...
Both immunosuppressive and cytoreductive effects of γ-irradiation contribute to engraftment allogeneic haematopoietic stem progenitor cells. We hypothesized that a release host cells from the niche prior conditioning would permit after less intensive conditioning. Administration AMD3100 SEW2871 on days -4 -2 followed by irradiation day -1 in non-myeloablative zebrafish transplant model resulted reduced radiation minimum dose 10 Gy 15 being sufficient for engraftment. Targeting SDF-1...
Abstract Funding Acknowledgements Type of funding sources: Public grant(s) – EU funding. Main source(s): European Research Council CDK4/6 inhibitor Ribociclib induces cardiotoxicity in dynamic engineered heart tissues, mitigated by E2F1 overexpression Background Ribociclib, a novel chemotherapeutic agent for metastatic breast cancer, functions as cyclin-dependent kinase 4/6 (CDK4/6) inhibitor1. Its mechanism action involves the CDK4/6-Rb-E2F1 pathway. use has very recently been associated...
Respiratory gas analyses are used to explore the influence of increased gravity on physiology breathing. Due mass, size and intricacy standard measurement systems, previous studies were limited a maximum acceleration of + 5 gz (head-to-foot direction). Furthermore, no in situ mainstream measurements oxygen carbon dioxide concentration possible under hypergravity conditions so far. This paper shows first study which demonstrates respiratory sensor system developed at Institute for Aerospace...
Abstract Signaling pathways of regulated necrosis, such as necroptosis and ferroptosis contribute to acute kidney injury (AKI), but the role pyroptosis is unclear. Pyroptosis mediated by pore-forming protein gasdermin D (GSDMD). Here, we report a specific pattern GSDMD-protein expression in peritubular compartment mice that underwent bilateral ischemia reperfusion (IRI). Along similar lines, GSDMD whole lysates increased during first 84 hours following cisplatin-induced AKI. Importantly,...