A5100662734- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Seed Germination and Physiology
- Mesenchymal stem cell research
- Plant tissue culture and regeneration
- 3D Printing in Biomedical Research
- Viral Infectious Diseases and Gene Expression in Insects
- GABA and Rice Research
- Powdery Mildew Fungal Diseases
- RNA Interference and Gene Delivery
- Plant Genetic and Mutation Studies
- Genetic Mapping and Diversity in Plants and Animals
- Plant Physiology and Cultivation Studies
- Magnetic and Electromagnetic Effects
- Sperm and Testicular Function
- Wheat and Barley Genetics and Pathology
- RNA modifications and cancer
- Chromosomal and Genetic Variations
- Genetics, Aging, and Longevity in Model Organisms
- Advanced oxidation water treatment
- Venomous Animal Envenomation and Studies
- Cholesterol and Lipid Metabolism
- Water Treatment and Disinfection
Anhui Normal University
2024
Wannan Medical College
2024
Yangzhou University
2015-2023
Beijing Forestry University
2008-2021
Zhongnan Hospital of Wuhan University
2021
Wuhan University
2021
Hubei Provincial Women and Children's Hospital
2021
Zhejiang University
2006-2019
Poultry Research Institute
2019
Southern Medical University
2017
Camellia oleifera Abel. is an important woody oil species; however, the shortage of rapid and industrialized seedling culture a large constraint on development tea industry. Somatic embryogenesis (SE) one main powerful biotechnological tools for plant mass regeneration, but largely unknown SE in C. limits scale production clonal plants. In this study, we described high-efficiency system via direct indirect pathways investigated effect genotype, explant age phytohormones SE. pathway, somatic...
Maspin (Mp) is a member of the serpin family with inhibitory functions against cell migration, metastasis and angiogenesis. To identify its role in embryonic development vivo, we generated maspin knockout mice by gene targeting. In this study, showed that homozygous loss expression was lethal at peri-implantation stage. specifically expressed visceral endoderm after implantation; deletion interfered formation endodermal layer, thereby disrupting morphogenesis epiblast. vitro, ICM Mp(-/-)...
Smooth muscle cell (SMC) differentiation and dedifferentiation play a critical role in the pathogenesis of cardiovascular diseases. The lack good simple vitro SMC system has hampered progress field for years. generation such an would be invaluable exploring molecular mechanisms dedifferentiation. Recently, establishment induced pluripotent stem (iPS) cells offered novel therapeutic strategy to generate patient-specific lines. Here we have investigated whether iPS are able differentiate into...
Nitric oxide (NO) is an important signaling molecule involved in many physiological processes plants. generation and flavonoid accumulation are two early reactions of plants to ultraviolet-B (UV-B) irradiation. However, the source UV-B-triggered NO role UV-B-induced not fully understood. In order evaluate origin generation, we examined responses nitrate reductase (NR) activity expression levels NIA1 NIA2 genes leaves Betula pendula Roth (silver birch) seedlings UV-B The data show that...
Here, we conducted the first large-scale leaf phosphoproteome analysis of two bread wheat cultivars by liquid chromatography-tandem mass spectrometry. Altogether, 1802 unambiguous phosphorylation sites representing 1175 phosphoproteins implicated in various molecular functions and cellular processes were identified gene ontology enrichment analysis. Among phosphoproteins, 141 contained 3-10 sites. The located more frequently N- C-terminal regions than internal regions, ∼70% outside conserved...
The allogeneic transplantation of primordial germ cells (PGCs) derived from somatic overcomes the limitation avian cloning. Here, we transdifferentiate chicken embryo fibroblasts (CEFs) black feathered Langshan chickens to PGCs and transplant them into White Plymouth Rock embryos produce viable offspring with characteristics inherited donor. We express Oct4/Sox2/Nanog/Lin28A (OSNL) reprogram CEFs induced pluripotent stem (iPSCs), which are further differentiate by BMP4/BMP8b/EGF. DNA...
Background The incidence of smartphone addiction has been widely studied, but the research on relationship between and psychological distress internet gaming disorder is limited. This study investigated characteristics prevalence its relation with disorder. Furthermore, it provides scientific basis for intervention measures in schools, families, society. Methods A random cluster sampling method was applied to investigate 656 medical students from grades 1 4 at Wannan Medical College Anhui...
Abstract Microenvironment (or niche)-providing chemokines regulate many important biological functions of tissue-specific stem cells. However, to what extent influence human pluripotent cells (hPSCs) is not yet completely understood. In this study, we applied protein array screen found within the cytokine pool in culture supernatant hPSCs. Our results showed that were predominant components, and came from three sources: hPSCs, feeder cells, media. Chemotaxis analysis IL-8, SDF-1α, IP-10...
Abstract This research was conducted to determine the optimum moisture content (MC) that gave maximum longevity seeds. Three species were used represent seeds with different dry matter reserves, which gives them sorption properties: maize ( Zea mays L.), elm Ulmus pumila L.) and safflower Carthamus tinctorius L.). The of elm, safflower, embryos MC ranging from 0.00–0.15 g H 2 O/g weight (DW) stored at 35 °C for periods time. results showed seed embryo storage varied between (0.057 DW...
Bone morphogenetic protein 4 (BMP4) has been reported to regulate adipose development, but its role in preadipocyte proliferation not explored vitro. Here, we investigated the effect of BMP4 on chicken using immortalized preadipocytes (ICP1 cells) as a cell model. We report that expression increases during proliferation. Overexpression and knockdown promotes inhibits proliferation, respectively. In addition, overexpression decreased number at G0/G1 phase cycle, increased proportion cells S...
Smooth muscle cells (SMCs) are important cell type for regenerative medicine. Previous studies showed that retinoic acid (RA) induces differentiation of SMCs from monolayer-cultured embryonic stem (ESCs) with high efficiency. However, the underlying mechanisms still poorly defined. Here, we identified Wnt signaling as a primary regulator RA-induced ESC differentiation. The activation inhibited epithelial-mesenchymal transition during differentiation, leading to inhibition SMC and promoting...
The MAPK/Erk signaling pathway is considered as a key regulator of the pluripotency and differentiation embryonic stem (ES) cells, while dual-specificity protein phosphatases (DUSPs) are negative regulators MAPK. Although DUSPs potential embryogenesis regulators, their functions in regulation ES cell have not been demonstrated. present study revealed that Dusp5 was expressed mouse (mES) cells its expression correlated with undifferentiated state these cells. Exogenous enhanced mES...
Mouse embryonic stem cells (mESCs) have played a key role in the newly emerging fields of cell research. The traditional derivation and culture mESCs been based on use mouse fibroblasts (MEFs) treated with exogenous leukemia inhibitory factor (LIF). However, rapid senescence MEFs, coupled high cost LIF, has significantly hampered widespread Thus, we present novel LIF-free system for general applications, comprising fibroblast-like derived from rabbit spleen (RSFs). We demonstrated that...
An ultraviolet/biological (UV/B) reactor was used to treat the secondary effluent of wastewater treatment plants (WWTPs).
Somatic cells can be reprogrammed into induced pluripotent stem (iPSCs) in vitro. Previously, a lentivirus induction strategy of introducing Oct4, Sox2, Nanog and Lin28 (OSNL) the iPSC process has been shown as possible way to produce chicken iPSCs from embryonic fibroblasts, but efficiency this method was found significantly limiting. In order help resolve obstacle, study seeks clarify associated regulation mechanisms optimizes reprogramming iPSCs. This showed that glycolysis expression...