A5054126797- Advanced biosensing and bioanalysis techniques
- Biochemical Analysis and Sensing Techniques
- Olfactory and Sensory Function Studies
- Receptor Mechanisms and Signaling
- Advanced Chemical Sensor Technologies
- Biosensors and Analytical Detection
- Protein purification and stability
- Neurobiology and Insect Physiology Research
- Advanced Biosensing Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Infant Nutrition and Health
- Electrochemical sensors and biosensors
- Ginseng Biological Effects and Applications
- Glycosylation and Glycoproteins Research
- SARS-CoV-2 detection and testing
- Ion Channels and Receptors
- Probiotics and Fermented Foods
- Electrochemical Analysis and Applications
- Bacteriophages and microbial interactions
- Neuroscience and Neuropharmacology Research
- Nicotinic Acetylcholine Receptors Study
- RNA and protein synthesis mechanisms
- Polysaccharides and Plant Cell Walls
- Animal health and immunology
- Pharmaceutical and Antibiotic Environmental Impacts
Tianjin University of Commerce
2014-2023
State Key Laboratory of Food Science and Technology
2015-2018
Tianjin University of Science and Technology
2016-2018
State Council of the People's Republic of China
2015
Tianjin People's Hospital
2015
How do functional foods affect human health? To answer this question it is important to understand what happens when food digested and taken up by the gastrointestinal (GI) tract. The gut a selective nutrient absorption system most signal transduction information exchange within body. It acts as transducer, neuroendocrine sensor, an immunological recognition presentation system. also complex comprising number of signaling networks involving GI cells immobilized in organs or transported...
The aim of this study was to determine the interaction between human umami receptor hT1R1 and a ligand while avoiding cross-talk among various signal pathways in cells. modified mounted onto amplification system on glassy carbon electrode surface, response current towards four ligands (sodium glutamate (MSG), disodium inosinate (IMP), guanylate (GMP), succinate (SUC)) measured. allosteric constants receptor-ligand were calculated by method sensing kinetics, results indicated that ability...
In the current study, a novel double-layer gold nanoparticles-electrochemical immunosensor electrode immobilized with tetrahydrocannabinol (THC) antibody derived from Balb/c mice was developed. To increase fixed quantity of antibodies and electrochemical signals, an biosensing signal amplification system utilized nanoparticles-thionine-chitosan absorbing horseradish peroxidase (HRP). addition, transmission electron microscope (TEM) used to characterize nanogold solution. evaluate quality...
Casein glycomacropeptide (GMP) is known to promote the in vitro growth of Bifidobacteria and Lactobacilli. In this paper, we used conven- tional culture techniques fluorescent situ hybridization (FISH) investigate effect casein GMP on mice fecal microbiota. The population structure intestinal microbiota, including Lactobacillus, Bifidobacteria, Enterococcus, coliforms Enterobacteriaceae, was tested compared. After consecutive administration for 15 days, numbers Lactobacillus increased...
In this study, an electrochemical sensor was developed by immobilizing colon cancer and the adjacent tissues (peripheral healthy on both sides of tumor) used to investigate receptor sensing kinetics glucose, sodium glutamate, disodium inosinate, lactate. The results showed that electrical signal triggered ligand-receptor interaction presented hyperbolic kinetic characteristics similar enzyme with its substrate. indicated activation constant values tissue differed two orders magnitude for...
In this paper, we utilized dimethylhydrazine (DMH)-induced colorectal cancer (CC) model rats to explore the effects of casein glycomacropeptide (CGMP) on cancer. Rats with CC were orally administrated 10, 50, or 100 mg/kg bw d CGMP, same volume phosphate-buffered saline for 15 weeks. The total numbers aberrant crypt foci (ACF) and crypts per focus in colon scored using a light microscope at low magnification after was stained methylene blue solutions. methylation level DNA extracted from...
The G protein cascade amplification system couples with several receptors to sense/amplify the cellular signal, implying universal application. In order explore whether GPCRs can trigger signal in tissues/cells from different species, bombykol receptor was isolated and purified antennas of male Bombyx mori, which subsequently self-assembled on cell membrane rat taste buds/rat vomeronasa/catfish tentacles/taste bud tissues rabbits/pig/cattle those lacking endogenous receptor, followed by...
Purpose – This paper aims to provide a detailed review of weak interaction biosensors and several common biosensor methods for magnifying signals, as well judiciously guide readers through selecting an appropriate detecting system signal amplification method according their research application purpose. Design/methodology/approach classifies the interactions between biomolecules, summarizes used in design compares performance different kinds biosensors. It highlights potential...
Endogenous and exogenous estrogens are widely present in food packaging, high levels of natural the misuse or illegal use synthetic can lead to endocrine disorders even cancer humans. Therefore, it is consequently important accurately evaluate presence food-functional ingredients toxins with estrogen-like effects. In this study, an electrochemical sensor based on G protein-coupled estrogen receptors (GPERs) was fabricated by self-assembly, modified double-layered gold nanoparticles, used...