The most important objective of the present study was to explain why cationic lipid (CL)-mediated delivery plasmid DNA (pDNA) is better than that linear in gene therapy, a question that, until now, has remained unanswered. Herein for first time we experimentally show different types CLs, pDNA, contrast DNA, compacted with large amount its counterions, yielding lower effective negative charge. This feature been confirmed through number physicochemical and biochemical investigations....

Lipoplexes formed by the pEGFP-C3 plasmid DNA (pDNA) and lipid mixtures containing cationic gemini surfactant of 1,2-bis(hexadecyl dimethyl ammonium) alkanes family referred to as C16CnC16, where n=2, 3, 5, or 12, zwitterionic helper lipid, 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE) have been studied from a wide variety physical, chemical, biological standpoints. The study has carried out using several experimental methods, such zeta potential, gel electrophoresis, small-angle...

Background and aims Obesity is linked to elevated levels of inflammatory serum markers such as C-reactive protein (CRP), interleukin-6 (IL-6), tumor necrosis factor alpha (TNFa). Adiponectin resistin are adipokines related obesity. It has been described that adipose tissue presents a high production secretion these diverse pro-inflammatory molecules, which may have local effects on the physiology fat cells well systemic other organs. Our aim was evaluate impact lifestyle modifications, by...

Lipoplex-type nanoaggregates prepared from pEGFP-C3 plasmid DNA (pDNA) and mixed liposomes, with a gemini cationic lipid (CL) [1,2-bis(hexadecyl imidazolium) alkanes], referred as (C16Im)2Cn (where Cn is the alkane spacer length, n = 2, 3, 5, or 12, between imidazolium heads) DOPE zwitterionic lipid, have been analyzed by zeta potential, gel electrophoresis, SAXS, cryo-TEM, fluorescence anisotropy, transfection efficiency, confocal microscopy, cell viability/cytotoxicity experiments to...

Lipoplex nano-aggregates have been analyzed through biophysical characterization (electrostatics, structure, size and morphology), biological studies (transfection efficiency cell viability) in five cancer lines. Lipoplexes were prepared from pEGFP-C3 plasmid DNA (pDNA) mixed liposomes, constituted by a zwitterionic lipid (DOPE) gemini cationic (GCL) synthesized this work, [bis(hexadecyl dimethyl ammonium) oxyethylene], referred to as (C16Am)2(C2O)n, (where n is the oxyethylene spacer...

Abstract Background A major bottleneck in drug delivery is the breakdown and degradation of system through endosomal/lysosomal network host cell, hampering correct interest. In nature, bacterial pathogen Listeria monocytogenes has developed a strategy to secrete Listeriolysin O (LLO) toxin as tool escape eukaryotic lysosomal upon infection, allowing it grow proliferate unharmed inside cell. Results As “proof concept”, we present here use purified His-LLO H311A mutant protein its conjugation...

Easy and direct BODIPY post-multifunctionalization without photophysics interference for the rapid design synthesis of functional organic dyes, including forefront CPL-bright chiral-at-boron BODIPYs.

The BODIPY family of organic dyes has emerged as a cornerstone in photonics research development, driving innovation and advancement various fields high socio-economic interest. However, the majority of...

Lipoplexes constituted by calf-thymus DNA (CT-DNA) and mixed cationic liposomes consisting of varying proportions the lipid 3β-[N-(N',N'-dimethylaminoethane)-carbamoyl]cholesterol hydrochloride (DC-Chol) zwitterionic lipid, 1,2-dioleoyl-sn-glycero-3-phosphoetanolamine (DOPE) have been analyzed means electrophoretic mobility, SAXS, fluorescence anisotropy experiments, as well theoretically calculated phase diagrams. Both experimental theoretical studies run at several liposome lipoplex...

Magnetic silica nanoparticles show great promise for drug delivery. The major advantages correspond to their magnetic nature and ease of biofunctionalization, which favors ability interact with cells tissues. We have prepared DNA fragments attached on previously polyelectrolyte-primed surface. remarkable feature these materials is the compromise between positive charges polyelectrolytes negative DNA. This dual-agent formulation dramatically changes overall cytotoxicity chemical degradation...

Lipoplexes of plasmid DNA and mixed liposomes, with a gemini cationic lipid the 1,2-bis(hexadecyl imidazolium) oxyethylene series, improves their biological activity.

Lipoplexes constituted by plasmid DNA pEGFP-C3 (pDNA) or linear double-stranded calf thymus (ctDNA) and mixed cationic liposomes consisting of several percentages the lysine derived lipid C6(LL)2 zwitterionic 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) have been analyzed both experimental theoretical approaches. Experimental studies, electrophoretic mobility/zeta potential, small angle X-ray scattering (SAXS), cryogenic transmission electron microscopy (cryo-TEM), negatively stained...

The mixed system consisting of two anionic surfactants identical headgroups but with 10 and 12 carbon atoms on the hydrophobic tail, sodium decanoate (C10Na) dodecanoate (C12Na), has been studied in aqueous solution at 298.15 K by means conductivity fluorescence spectroscopy experiments from a theoretical point view. monomeric micellar phases aggregates were analyzed through experimental determination total critical micelle concentration, cmc*, degree ionization micelle, β, aggregation...

A double approach, experimental and theoretical, has been followed to characterize from a physicochemical standpoint the compaction process of DNA by means cationic colloidal aggregates. The vectors are liposomes constituted mixture novel lipid, 1,2-dioleoyl-sn-glycero-3-ethylphosphocholine (chloride salt) (DOEPC) zwitterionic 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE). wide variety high precision techniques have used carry out analysis: electrophoretic mobility, small-angle...

The design of efficient drug delivery vectors requires versatile formulations able to simultaneously direct a multitude molecular targets and bypass the endosomal recycling pathway cells. Liposomal-based need decoration lipid surface with specific peptides fulfill functional requirements. unspecific binding is often accompanied uncontrolled thus preventing mechanisms successful therapy.We present simple synthesis anchor cysteine-terminal thiol-reactive lipids for adequate quantitative...

Organo-modified mesoporous silica nanoparticles, loaded with ibuprofen into the pores and functionalized on external surface a stable Gd(<sc>iii</sc>)–DOTA-monoamide chelate, were prepared explored as potential theranostic probes.

(1) Background and aims: Obesity high body max index (BMI) have been linked to elevated levels of inflammation serum markers such as C-reactive protein (CRP), interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-alpha), adiponectin, resistin. It has described that adipose tissue presents a production secretion these diverse pro-inflammatory molecules, which may local effects on the physiology fat cell also systemic other organs. Our aim was evaluate impact lifestyle modifications,...

The redox microenvironment within a cell graft can be considered as an indicator to assess whether the is metabolically active or hypoxic. We present redox-responsive MRI probe based on porous silica microparticles whose surface has been decorated with Gd-chelate through disulphide bridge. Such are designed interspersed therapeutic cells biocompatible hydrogel. onset of reducing conditions hydrogel paralleled by increased clearance Gd, that detected MRI.

Arterial stiffness is linked with the development of cardiovascular and noncardiovascular diseases. In clinical practice, measurement carotid-femoral pulse wave velocity (cf-PWV) has become a widely used study for assessment risk in elderly population. Our aim was to evaluate whether maintaining healthy life, based on Mediterranean Diet (MedDiet) regular practice physical activity, are associated arterial an elderly, metabolically overweight or obesity (MHOe) population.A transversal,...

The fluorescent dye 10-N-nonyl acridine orange (NAO) is widely used as a mitochondrial marker. NAO was reported to have cytotoxic effects in cultured eukaryotic cells when incubated at high concentrations. Although the biochemical response of NAO-induced toxicity has been well identified, underlying molecular mechanism not yet explored detail.We use optical techniques, including fluorescence confocal microscopy and lifetime imaging (FLIM) both model membranes built up giant unilamellar...

A very simple, small and symmetric, but highly bright, photostable functionalizable molecular probe for plasma membrane (PM) has been developed from an accessible, lipophilic clickable organic dye based on BODIPY.

Nonyl acridine orange (NAO) is a lipophilic and positively charged molecule widely used as mitochondrial fluorescent probe. NAO cytotoxic at micromolar concentration might be potentially mitochondria-targeted drug for cancer therapy. However, the use of under in vivo conditions would compromised by unspecific interactions with off-target cells negatively proteins present bloodstream. To tackle this limitation, we have synthesized analogues carrying an imidazole group their specific binding...

Lipoplexes are non-viral vectors based on cationic lipids used to deliver DNA into cells, also known as lipofection. The positively charge of the hydrophilic head-group provides ability condensate negatively charged structured complexes. polar head can carry a large variety chemical groups including amines well guanidino or imidazole groups. In particular, gemini consist two positive heads linked by spacer with different length. As for hydrophobic aliphatic chains, they be unsaturated...