A5046570826- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- Molecular Biology Techniques and Applications
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Cancer Cells and Metastasis
- Chronic Lymphocytic Leukemia Research
- Monoclonal and Polyclonal Antibodies Research
- Cell Image Analysis Techniques
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Adipokines, Inflammation, and Metabolic Diseases
- Nuclear Receptors and Signaling
- Lymphoma Diagnosis and Treatment
- Fiscal Policy and Economic Growth
- Lipid metabolism and disorders
- vaccines and immunoinformatics approaches
- GaN-based semiconductor devices and materials
- Metal and Thin Film Mechanics
- Transgenic Plants and Applications
- Acute Myeloid Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Anesthesia and Pain Management
- Genetics, Bioinformatics, and Biomedical Research
Seoul National University
2015-2023
Chungnam National University
2022
Korea Research Institute of Bioscience and Biotechnology
2018-2022
Korea University of Science and Technology
2018
Jeonbuk National University
2005
Kosin University
2004
Abstract Neoantigens are ideal targets for cancer immunotherapy because they expressed de novo in tumor tissue but not healthy and therefore recognized as foreign by the immune system. Advances next-generation sequencing bioinformatics technologies have enabled quick identification prediction of tumor-specific neoantigens; however, only a small fraction predicted neoantigens immunogenic. To improve predictability immunogenic neoantigens, we developed silico neoantigen workflows VACINUS pMHC...
Abstract Writing DNA plays a significant role in the fields of synthetic biology, functional genomics and bioengineering. clones on next-generation sequencing (NGS) platforms have potential to be rich cost-effective source sequence-verified DNAs as precursor for writing. However, it is still very challenging retrieve target clonal from high-density NGS platforms. Here we propose an enabling technology called ‘Sniper Cloning’ that enables precise mapping clone features non-contact rapid...
Determining mutational landscapes in a spatial context is essential for understanding genetically heterogeneous cell microniches. Current approaches, such as Multiple Displacement Amplification (MDA), offer high genome coverage but limited multiplexing, which hinders large-scale genomic studies. Here, we introduce barcoded MDA (bMDA), technique that achieves high-coverage analysis of low-input DNA while enhancing the multiplexing capabilities. By incorporating barcodes during MDA, bMDA...
Spatial mapping of genomic data to tissue context in a high-throughput and high-resolution manner has been challenging due technical limitations. Here, we describe PHLI-seq, novel approach that enables isolation genome-wide sequence analysis single cells or small numbers construct maps within cancer relation the images phenotypes cells. By applying reveal heterogeneity breast tissues at high resolution map landscape their corresponding spatial locations 3D tumor mass.
Abstract If a large amount of polymer radicals remain trapped after the irradiation ultrahigh molecular weight polyethylene (UHMWPE), may result in significant alteration its physical properties during long‐term shelf storage and implantation. An electron spin resonance spectroscopic study was undertaken to investigate remaining free UHMWPE beam up 500 kGy air an N 2 environment. Heat treatment employed at 110 145°C for various periods time decay radicals. The were rapidly decayed 1 h...
Abstract Epithelial ovarian cancer (EOC) is a silent but mostly lethal gynecologic malignancy. Most patients present with malignant ascites and peritoneal seeding at diagnosis. In the study, we used laser-aided isolation technique to investigate clonal relationship between primary tumor spheroids found in of an EOC patient. Somatic alteration profiles cancer-related genes were determined for eight spatially separated samples from tissues ten using next-generation sequencing. We observed high...
The demand for patterning functional materials precisely on surfaces of stimuli-responsive devices has increased in many research fields. In situ polymerization technology is one the most convenient ways to place a desired location with micron-scale accuracy. To fabricate surfaces, controlling concentration material much as important micropatterning them. However, and simultaneously requires an additional process, such preparing multiple co-flow microfluidic structures numbers solutions...
Staphylococcal enterotoxin B (SEB) is a potent bacterial toxin that causes inflammatory stimulation and toxic shock, thus it necessary to detect SEB in food environmental samples. Here, we developed sensitive immunodetection system using monoclonal antibodies (mAbs). Our study the first employ baculovirus expression vector (BEVS) produce recombinant wild-type SEB. BEVS facilitated high-quantity pure production from suspension-cultured insect cells, produced was characterized by mass...
BackgroundLittle is known about the mutational frequency of myeloid differentiation factor 88 (MYD88) and C-X-C chemokine receptor type 4 (CXCR4) corresponding characteristics in Asian individuals afflicted with Waldenström macroglobulinemia (WM). We investigated WM according to status MYD88/CXCR4, attempted determine lineage commitment among hematopoietic cells by MYD88L265P single-cell sequencing on bone marrow (BM) smear slides.Materials MethodsCXCR4 mutations (muts) were detected using...
Purpose: Cancer stem like cells (CSCs), with unlimited self-renewal potential and other cell characteristics, occur in several cancers including hepatocellular carcinoma (HCC).Although CSCs can initiate tumors, malignant proliferation, relapse multidrug resistance, the ways how to activate them still remain unknown.This study aims evaluate whether CSC acquire tumorigenic characters under tumor hypoxia, analyzed by microarray analysis.Materials Methods: were purified from HCC patients...
Analyzing archived peripheral blood smears is a potential route towards gaining cell morphology and genome information of types from various diseases. Yet, acquiring whole morphologically targeted cells was difficult, especially for rare types. The main causes such difficulty were the inevitable usage stains leading to amplification inhibition, insufficient isolation performance previously introduced laser microdissection (LMD) techniques. Here, we introduce new laser-based technique (WGA)...
This study employs panel data from 2000 to 2019 examine and analyze the impact of foreign aid on growth in six South Asian nations, which are Bangladesh, Bhutan, India, Nepal, Pakistan, Sri Lanka. The motivation this is furnish empirical support for resolving ongoing discourse relationship between economic growth. To end, article three regression models: pooled ordinary least-squares, random effect, fixed estimates them using White cross-sectional standard errors covariance. results reveal...
A spatially resolved analysis of the heterogeneous cancer genome, in which data are connected to three-dimensional space a tumour, is crucial understand biology and clinical impact heterogeneity on patients. However, despite recent progress transcriptomics, spatial mapping genomic high-throughput high-resolution manner has been challenging due current technical limitations. Here, we describe novel approach, phenotype-based laser-aided isolation sequencing (PHLI-seq), enables single-cell or...
Abstract Background Epithelial ovarian cancer (EOC) is a silent but mostly lethal gynecologic malignancy. Most patients present with malignant ascites and peritoneal seeding at diagnosis. In the study, we used laser-aided isolation technique to investigate clonal relationship between primary tumor spheroids found in of an EOC patient. Somatic alteration profiles cancer-related genes were determined for eight spatially separated samples from tissues ten using next-generation sequencing....
Although cervical epidural steroid injection with local anaesthetic is considered a safe technique and widely practiced, complications may occur. We report patient that experienced unexpected hemiparesis due to upper cord injury involving the medulla during block steroid. The lost her consciousness just after assisted ventilation was required. Left hemiplegia also present. After four months of follow-up, she able walk without help for short distance, but swallowing difficulty, hoarseness,...