A5055522361- Immune cells in cancer
- Pregnancy and preeclampsia studies
- Angiogenesis and VEGF in Cancer
- Birth, Development, and Health
- Atherosclerosis and Cardiovascular Diseases
- Cancer, Lipids, and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Neonatal Respiratory Health Research
- Single-cell and spatial transcriptomics
- Zebrafish Biomedical Research Applications
- Congenital heart defects research
- Apelin-related biomedical research
- Mitochondrial Function and Pathology
- Advanced MRI Techniques and Applications
- MicroRNA in disease regulation
- Inflammasome and immune disorders
- Cardiac Imaging and Diagnostics
- Pregnancy and Medication Impact
- Nuclear Physics and Applications
- Cardiac Structural Anomalies and Repair
- Preterm Birth and Chorioamnionitis
- Systemic Lupus Erythematosus Research
- Electrostatic Discharge in Electronics
- Extracellular vesicles in disease
- Aortic Disease and Treatment Approaches
Pfizer (United States)
2024
Novartis (Germany)
2024
University of South Australia
2021-2024
The University of Adelaide
2020-2024
Royal Adelaide Hospital
2024
South Australian Health and Medical Research Institute
2019-2023
Abstract Colchicine is a broad‐acting anti‐inflammatory agent that has attracted interest for repurposing in atherosclerotic cardiovascular disease. Here, we studied its ability at human equivalent dose of 0.5 mg/day to modify plaque formation and composition murine atherosclerosis investigated actions on macrophage responses atherogenic stimuli vitro. In induced by high‐cholesterol diet, Apoe −/− mice treated with colchicine had 50% reduction aortic oil Red O + area compared saline control...
Abstract Magnetic resonance imaging (MRI) assessment of fetal blood oxygen saturation (SO 2 ) can transform the clinical management high‐risk pregnancies affected by growth restriction (FGR). Here, a novel MRI method assesses feasibility identifying normally grown and FGR fetuses in sheep is then applied to humans. scans are performed pregnant ewes at 110 140 days (term = 150d) gestation women 28 +3 ± +5 weeks measure feto‐placental SO . Birth weight collected and, sheep, measured with gas...
Abstract Babies born with fetal growth restriction (FGR) are at higher risk of developing cardiometabolic diseases across the life course. The reduction in substrate supply to fetus that causes FGR not only alters cardiac and structure but may have deleterious effects on metabolism function. Using a sheep model placental induce FGR, we investigated key metabolic functional markers be altered FGR. We also employed phase‐contrast magnetic resonance imaging MRI assess left ventricular output...
Maternal asthma is associated with increased rates of neonatal lung disease, and fetuses from asthmatic ewes have fewer surfactant-producing cells lower surfactant-protein B gene (SFTPB) expression than controls. Antenatal betamethasone increases surfactant production in preterm babies, we therefore tested this therapy experimental maternal asthma. Ewes were sensitised to house dust mite allergen, an phenotype induced by fortnightly allergen challenges; controls received saline. Pregnant...
Abstract The cellular origins of vasa vasorum are ill-defined and may involve circulating or local progenitor cells. We previously discovered that murine aortic adventitia contains Sca-1 + CD45 progenitors produce macrophages. Here we investigated whether they also vasculogenic. In aortas C57BL/6 mice, cells were localised to lacked surface expression endothelial markers (<1% for CD31, CD144, TIE-2). contrast, did show TIE-2 VEGFR2 in atherosclerotic ApoE −/− aortas. Although from aorta...
Intrauterine growth restriction (IUGR) is a result of limited substrate supply to the developing fetus in utero, and can be caused by either placental, genetic or environmental factors. Babies born IUGR have poor long-term health outcomes, including being at higher risk cardiovascular disease. Limited not only changes structure heart but may also affect metabolism function heart. We utilised two imaging modalities, two-photon microscopy phase-contrast MRI (PC-MRI), assess alterations cardiac...
The adult mammalian heart is unable to undergo cardiac repair, limiting potential treatment options after damage. However, the fetal capable of repair. In preparation for birth, cardiomyocytes (CMs) major maturational changes that include exit from cell cycle, hypertrophic growth, and mitochondrial maturation. timing regulation such events in large mammals not fully understood. present study, we aimed assess this critical CM transition period using pigs as a preclinically relevant model....
Converging evidence indicates that extra-embryonic yolk sac is the source of both macrophages and endothelial cells in adult mouse tissues. Prevailing views are these embryonically derived maintained after birth by proliferative self-renewal their differentiated states. Here we identify clonogenic endothelial-macrophage (EndoMac) progenitor adventitia embryonic postnatal aorta, independent Flt3-mediated bone marrow hematopoiesis derive from an early CX
Eukaryotic elongation factor 2 kinase (eEF2K) is an atypical protein that controls synthesis in cells under stress. Although well studied cancer, less known about its roles chronic inflammatory diseases. Here, we examined regulation of macrophage cholesterol handling the context atherosclerosis. eEF2K mRNA expression and activity were upregulated murine bone marrow-derived macrophages (BMDMs) exposed to oxidized low-density lipoprotein (oxLDL). When incubated with oxLDL, BMDMs from knockout...
We investigated the accuracy of cardiac MRI for detecting myocardial infarction (MI) induced by coronary artery ligation surgery in a sheep model. 3D-inversion recovery (IR) FLASH sequence and first-pass perfusion imaging was feasible provided accurate information about size location MI immediately after at 15-day follow-up. The IR-FLASH detected infarcts (91.2% sensitivity, 99.9% specificity) compared with gold-standard pathology staining findings. This may prove useful preclinical research...
Abstract Background Macrophages and endothelial cells share an intimate relationship during neovessel formation in different pathophysiological conditions. Recent studies have determined that some tissues, both cell types are derived embryonically from yolk sac (YS) progenitor maintained postnatally without contribution circulating sources. The mechanism by which this local “self-maintenance” occurs is unknown. Purpose We previously identified mouse arteries contain macrophage their...
Background: Yolk sac (YS) progenitors are a source of macrophages and endothelial cells in some tissues that thought to be maintained postnatally by self-renewal their differentiated states. How this is achieved remains poorly understood. Methods Results: Single-cell digests from mouse aortas selectively formed macrophage colony-forming units (CFU-M) methylcellulose, which self-renewed secondary cultures single cells. CFU-M comprised homogeneous population...