The ethylene response factor (ERF) family is one of the largest plant-specific transcription families, playing an important role in plant development and to stresses. ERF76 gene a member poplar ERF family. First, we validated that expressed leaf root tissues responsive salinity stress. We then successfully cloned cDNA fragment containing open reading frame from di-haploid Populus simonii × nigra proved protein targeted nucleus. Finally, transferred into same clone by Agrobacterium-mediated...

Abstract Background: Health education has been considered as the effectiveness method to increase self-care skills of diabetes patients. However, limited studies investigate association health via Wechat platform on increased basic and glycemic control rate in patients with type 2 diabetes. Methods: A total number 120 were randomized into intervention (health by plus usual care) group (usual care). Biochemical parameters including fasting plasma glucose (FPG), 2-hour (2hPG), glycosylated...

Drought is one of the main environmental factors limiting plant growth and development. The AP2/ERF transcription factor (TF) ERF194 play key roles in poplar drought-stress tolerance. However, physiological mechanism remains to be explored. In this study, ERF194-overexpression (OX), suppressed-expression (RNA interference, RNAi), non-transgenic (WT) clone 717 were used study physiology role drought Morphological methods systematically analyze status, antioxidant enzyme activity,...

Cadmium (Cd) is a nonessential microelement and low concentration Cd2+ has strong toxicity to plant growth. Plant metallothioneins, class of molecular, cystein(Cys)-rich heavy-metal binding proteins, play an important role in both metal chaperoning scavenging reactive oxygen species (ROS) with their large number cysteine residues therefore, protect plants from oxidative damage. In this study, metallothionein gene, TaMT3, isolated Tamarix androssowii was transformed into tobacco (Nicotiana...

The Heat Shock Factor (HSF) transcription factor family plays crucial roles in plant growth and development, as well protecting against adverse stresses. However, studies on the functions regulatory mechanisms of HSF genes are limited poplar. Here, we identified classified 30 factors Populus trichocarpa based recent genomic data annotation information conducted a comprehensive analysis these proteins, including phylogenetic physicochemical properties analysis, domain characterization,...

Superoxide dismutase (SOD) is widely present in plants and plays a crucial role defending against oxidative stress preventing tissue damage. This study discovered that the

The aggravation of soil salinization limits the growth and development plants. AP2/ERF transcription factors (TFs) have been identified play essential roles in plant stress response processes. In this study, function PagERF16 was detected using overexpressing (OX) RNAi transgenic poplar 84K hybrids. Plant growth, stomatal conductance, antioxidant enzymes activity, co-expressed TFs were analyzed morphological, physiological, molecular methods. OX showed a more robust lateral root system with...

Abstract The water deficits limit the growth and development of agricultural forest organisms. AP2/ethylene response factor (ERF) family has been identified as one largest plant-specific transcription factors (TFs) essential for plant stress response. function PtaERF194 in drought tolerance was detected overexpression (OX) RNA interference (RNAi) transgenic poplar 717 hybrids (Populus tremula × Populus alba). Plant growth, stem vessels, water-use efficiency (WUE), chlorophyll content...

Abstract Poplar is one of the main urban and rural greening shade tree species in northern hemisphere, but its growth development always restricted by salt stress. R2R3-MYB transcription factor family commonly involved many biological processes during plant stress endurance. In this study, PagMYB151 (Potri.014G035100) members related to expressed both nucleus cell membrane was cloned from Populus alba × P. glandulosa perfect tolerance mechanism. Morphological physiological indexes regulated...

Salt stress is one of the major abiotic stresses that limits plant growth and development. The MYB transcription factor family plays essential roles in development, as well tolerance processes. In this study, cDNA 84K poplar (Populus abla × Populus glandulosa) was used a template to clone full length PagMYB205 gene fragment, transgenic lines with overexpression (OX) or inhibited expression (RNAi, RNA interference) were cultivated. role development salt detected using morphological...

Research Highlights: Sequence phylogeny, genome organisation, gene structure, conserved motifs, promoter cis-element and expression profiling of poplar NACs related to salt stress were detected. In addition, two salt-induced was analysed. Background Objectives: NAC transcription factor (TF) proteins are involved in a wide range functions during plant development stress-related endurance processes. To understand the function Populus TFs tolerance, we characterised structure profile total 289...

Comparative plastomics can be used to explicitly dissect various types of plastome variation. In the present study, variation pattern Ziziphus jujuba var. spinosa (also called sour jujube) and its phylogenomic relationship with Chinese jujube were investigated. Plastomes 21 individuals sequenced assembled. The length plastomes ranged between 159399 161279 bp. exhibited collinearity structure, gene order content. most divergent regions located in intergenic spacers, such as trnR-UCU-atpA...

GATA transcription factors are ubiquitously present in eukaryotic organisms and play a crucial role multiple biological processes, such as plant growth, stress response, hormone signaling. However, the study of poplar is currently limited to small number proteins, despite their evident functional importance. In this investigation, we utilized most recent genome annotation stringent criteria identify 38 factor genes poplar. Subsequently, conducted comprehensive analysis gene family,...

176 ERF genes from Populus were identified by bioinformatics analysis, 13 of these in di-haploid simonii × P. nigra investigate real-time RT-PCR, the results demonstrated that highly responsive to salt stress, drought stress and ABA treatment, all expressed root, stem, leaf tissues, whereas their expression levels markedly different various tissues. In roots, PthERF99, 110, 119, 168 primarily downregulated under treatment but specifically upregulated high condition. Interestingly, poplar...

In order to study genetic diversity of white birch (Betula platyphylla), 544 primer pairs were designed based on the genome-wide Solexa sequences. Among them, 215 showed polymorphism between five genotypes and 111 that presented clear visible bands in genotyping 41 plants collected from 6 different geographical regions. A total 717 alleles obtained at loci with a range 2 12 per locus. The results statistic analysis polymorphic frequency ranged 17% 100% mean 55.85%; information content (PIC)...

Abstract Background Risk stratification of patients with acute myocardial infarction (AMI) is great clinical significance. Hypothesis The present study aimed to establish an optimized risk score predict short‐term (6‐month) death among rural AMI from China. Methods We enrolled 6581 and extracted relevant data. Patients were divided chronologically into a derivation cohort ( n = 5539), the multivariable prediction model, validation 1042), validate score. Results Six variables identified as...

Ethylene response factors (ERFs) are plant-specific transcription (TFs) that play important roles in plant growth and stress defense have received a great amount of attention recent years. In this study, seven ERF genes related to abiotic tolerance were identified plants the Populus genus. Systematic bioinformatics, including sequence phylogeny, genome organisation, gene structure, ontology (GO) annotation, etc. detected. Expression-pattern these analyzed using RT-qPCR cross validated...

MYB transcription factors (TF) are recognized as significant regulators in facilitating plant adaptation to various biotic and abiotic stresses. In order elucidate the involvement of TFs growth, development, salt tolerance poplar, PagMYB73A was cloned from 84K poplar (<italic>Populus alba × P. glandulosa</italic>) utilized target gene for generating overexpressing transgenic lines (OE-1 , OE-8 OE-12). Compared wild type (WT) plants under normal conditions, OE exhibited enhanced height...