A5088441940- Protease and Inhibitor Mechanisms
- S100 Proteins and Annexins
- Blood Coagulation and Thrombosis Mechanisms
- Signaling Pathways in Disease
- Cell Adhesion Molecules Research
- Adipokines, Inflammation, and Metabolic Diseases
- Galectins and Cancer Biology
- Peptidase Inhibition and Analysis
- Phagocytosis and Immune Regulation
- Cancer-related gene regulation
- Ubiquitin and proteasome pathways
- Adipose Tissue and Metabolism
- Antimicrobial Peptides and Activities
- Wound Healing and Treatments
- Invertebrate Immune Response Mechanisms
- Regulation of Appetite and Obesity
- Antioxidant Activity and Oxidative Stress
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Tissue Engineering and Regenerative Medicine
- Tendon Structure and Treatment
- 3D Printing in Biomedical Research
- Sepsis Diagnosis and Treatment
- Pluripotent Stem Cells Research
- Glycosylation and Glycoproteins Research
- Pancreatitis Pathology and Treatment
Scripps Research Institute
2014-2025
Scripps (United States)
2023
Scripps Institution of Oceanography
2023
King's College London
2016
Centro Cardiologico Monzino
2016
Institute of Experimental Cardiology
2016
Imperial College London
2016
University of Florence
2016
University of Notre Dame
2006
Inflammation resolution is an active process that functions to restore tissue homeostasis. Clearance of apoptotic leukocytes by efferocytosis at inflammatory sites plays important role in inflammation and induces remarkable macrophage phenotypic functional changes. Here, we investigated the effects deletion either plasminogen (Plg) or Plg receptor, Plg-RKT, on inflammation. In a murine model pleurisy, numbers total mononuclear cells recruited pleural cavity were significantly decreased both...
Sepsis is a lethal syndrome characterized by systemic inflammation and abnormal coagulation. Despite therapeutic advances, sepsis mortality remains substantially high. Herein, we investigated the role of plasminogen/plasmin (Plg/Pla) system during sepsis. Plasma levels Plg were significantly lower in mice subjected to severe compared with nonsevere sepsis, whereas IL-6, marker severity, higher correlated negatively IL-6 both septic patients, plasminogen activator inhibitor-1 positively IL-6....
An emerging area of research has documented a novel role for the plasminogen activation system in regulation neurotransmitter release. Prohormones, secreted by cells within sympathoadrenal system, are processed plasmin to bioactive peptides that feed back inhibit secretagogue-stimulated Catecholaminergic prototypic prohormone-secreting cells. Processing prohormones is enhanced presence catecholaminergic cells, and enhancement requires binding plasmin(ogen) cellular receptors. Consequently,...
Abstract Wound healing is a complex physiologic process that proceeds in overlapping, sequential steps. Plasminogen promotes fibrinolysis and potentiates the inflammatory response during wound healing. We have tested hypothesis novel plasminogen receptor, Plg-R KT , regulates key steps Standardized burn wounds were induced mice time dependence of closure was quantified. Healing −/− significantly delayed proliferation phase. Expression cytokines dysregulated tissue. Consistent with cytokine...
The plasminogen activation system regulates the activity of serine protease, plasmin. role receptors in cancer progression is being increasingly appreciated as key players modulation tumor microenvironment. interaction with cells to promote requires presence proteins exposing C-terminal lysines on cell surface. Plg-RKT a structurally unique receptor because it an integral membrane protein that synthesized and binds via lysine exposed Here, we have investigated expression human breast tumors...
Plasminogen activation is markedly enhanced on cell surfaces. Cell‐bound plasmin plays a key role in migration. Plg‐R KT novel transmembrane plasminogen receptor induced when promonocytes differentiate. Here we investigated the of macrophage migration using an anti‐Plg‐R mAb that competes with for binding to cells. Chemotaxis was stimulated by MCP‐1 transwells U937 monocytoid cells expressed high levels . In presence plasminogen, decreased treatment compared isotype control. peritoneal...
Plg‐R KT is a novel transmembrane plasminogen receptor that binds via carboxy‐terminal lysine residue. In order to evaluate the role of in vivo we generated ‐/‐ mice using homologous recombination and backcrossed ten generations into C57BL/6J background. The were viable fertile. Among 2,340 littermates from heterozygous matings 611 +/+ (26%), 1,136 +/‐ (49%) 593 (25%). This distribution was not significantly different (P>0.05, Chi‐square test) expected Mendelian 1:2:1 ratios indicate...
The plasminogen receptor, Plg-RKT, is a unique cell surface receptor that broadly expressed in cells and tissues throughout the body. Plg-RKT localizes on surfaces promotes its activation to broad-spectrum serine protease, plasmin. In this study, we show overexpression of protects mice from high fat diet (HFD)-induced adipose metabolic dysfunction. During first 10 weeks HFD, body weights overexpressed (Plg-RKT-OEX) were lower than those control (CagRosaPlgRKT). After CagRosaPlgRKT...
A key step in thrombolysis is the interaction of plasminogen with substrate, fibrin. This results marked enhancement activation. To study this mechanism we developed a monoclonal antibody (mAb 109) that reacts fibrin‐bound presence ≥ 50‐fold molar excess soluble plasminogen. The objective our was to define epitope recognized by mAb 109 reports conformational change upon binding Plasminogen denatured urea and reduced alkylated followed digestion trypsin. Using western blotting specific...