A5045405072- Dysphagia Assessment and Management
- Ear Surgery and Otitis Media
- Bone Tissue Engineering Materials
- Tracheal and airway disorders
- Orthopedic Infections and Treatments
- Bacterial biofilms and quorum sensing
- Dental materials and restorations
- Voice and Speech Disorders
- Nasal Surgery and Airway Studies
- Dental Implant Techniques and Outcomes
- Mycobacterium research and diagnosis
- Sinusitis and nasal conditions
- Diamond and Carbon-based Materials Research
- Infectious Diseases and Mycology
- Esophageal and GI Pathology
- Orthopaedic implants and arthroplasty
- Amyotrophic Lateral Sclerosis Research
- Infection Control and Ventilation
- Gastrointestinal Bleeding Diagnosis and Treatment
- Otolaryngology and Infectious Diseases
- Tuberculosis Research and Epidemiology
- Airway Management and Intubation Techniques
- Metal and Thin Film Mechanics
- Salivary Gland Disorders and Functions
- Antimicrobial Resistance in Staphylococcus
Helsinki University Hospital
2014-2024
University of Helsinki
2014-2024
Kainuun keskussairaala
2008-2017
Hospital Universitario Fundación Jiménez Díaz
2007-2009
ABSTRACT To improve the microbiological diagnosis of device-related osteoarticular infections, we have developed a protocol based on sonication device samples, followed by concentration and inoculation sonicate in broad variety media quantitative manner. Sixty-six samples from 31 patients were included study (17 them with clinical infection). The procedure had sensitivity 94.1%, which is better than that conventional cultures (88.2%). One case contamination six cases unexpected positive...
The prevalence of human rhino-, entero-, and coronaviruses was investigated by RT-PCR in nasopharyngeal aspirates from 107 children without concurrent respiratory symptoms. were admitted to the hospital for elective surgery. parents filled a questionnaire about occurrence symptoms four weeks before two after rate viral detection 45% with related past or recent infection whereas 20% samples taken any infections positive picornavirus RNA, P = 0.008. Thirty-one (29%) 18% rhinovirus, 11%...
Hypothesis: An albumin coating on titanium implants will inhibit bacterial adhesion the implant surface. Background: Bacterial, protein, and platelet otologic tympanostomy tubes is a major reason for sequelae can eventually lead to removal. The role of in prevention protein surface has already been tested by authors. In present study authors examined vitro adherence Staphylococcus aureus Pseudomonas aeruginosa an albumin-coated uncoated Methods: Human serum (HSA)-coated surfaces were exposed...
Hydroxyapatite (HA) and biphasic calcium phosphate (BCP) ceramic materials are widely employed as bone substitutes due to their porous osteoconductive structure. Their porosity the lowering of surrounding pH a result surgical trauma may, however, predispose these bacterial infections. For this reason, influence on adherence common Gram-positive bacteria surfaces requires investigation. Mercury intrusion porosimetry measurements revealed that pore size distribution both bioceramics had,...
A study to evaluate the biofilm-development ability in three different media (Middlebrook 7H9, sterile tap water and PBS-5% glucose) was performed with 19 collection strains from 15 species on non-pigmented rapidly growing mycobacteria (NPRGM). microtiter plate assay developed percentage of covered surface wells days day 1 69. All were able develop biofilm all tested media. Middlebrook 7H9 showed fastest growth, followed by glucose. sigmoid growth curve detected both water. difference could...
We designed a study to determine the role of mastoid mucosal biofilm in chronic otitis media (COM).Biofilm formation has been found several airway infections. COM is associated with chronic, recalcitrant infection mucosa, and surgery often required.COM patients were divided into 2 groups: one suppurative (CSOM) cholesteatoma presence. All had involvement preoperative computed tomographic scan. The control group consisted undergoing cochlear implantation, no previous history media. Mastoid...
Abstract Staphylococci cause the majority of nosocomial implant‐related infections initiated by adhesion planktonic bacteria to implant surface. It was hypothesized that plasma accelerating filtered pulsed arc discharge method enables combination advantageous properties diamond with antisoiling polymers. Diamond‐like carbon polytetrafluoroethylene hybrid (DLC‐PTFE‐h) coating produced. The S. aureus ATCC 25923 (10 8 colony‐forming units/mL) surfaces diminished from 2.32%, 2.35%, and 2.57%...
Implantation of a biomaterial provides an adhesion substratum both to host cell integration and contaminating bacteria. We studied simultaneous competitive Staphylococcus aureus in serial 1:10 dilutions 108 colony forming units (CFU)/mL human osteogenic sarcoma (SaOS-2) or primary osteoblast (hOB) cells, 1x105 cells/mL, the surfaces titanium, polydimethylsiloxane polystyrene. The bacterial adherence proliferation, cytotoxicity production reactive oxygen species (ROS) were using fluorometric...
Abstract Staphylococcus aureus device‐related infection is a common complication in implantology. Bacterial adhesion on implant surfaces the initial step infective process. The aim was to develop method suitable for quantitative bacterial adherence studies and test new diamond‐like carbon (DLC) coating against commonly used metallic biomaterials with regards adhesion. Patterned silicon chips spots of tantalum, titanium, chromium, DLC were produced using ultraviolet lithography physical vapor...
Abstract The bacterial adherence onto different multifunctional silica‐based bioceramics has been evaluated. Staphylococcus aureus and epidermidis were chosen, as they cause the majority of implant‐related infections in this field. Two SiO 2 mesoporous materials (MCM‐41, SBA‐15), an ordered ‐CaO‐P O 5 glass (OMG), a biphasic magnetic bioceramic (BMB), incubated with S. for 90 min, subsequently sonicated to quantify number adhered bacteria on each material. It was found that (10 8 CFU/mL)...
<h3>Objective</h3>To determine the incidence of biofilm on vocal fold epithelium patients with chronic laryngitis. Bacteria can grow in protected by a glycoprotein mass. Recent studies have shown importance otolaryngologic infections. Because laryngitis is often recurrent and occasionally purulent, we hypothesized that it related.<h3>Design</h3>Prospective, controlled, blinded study. Epithelial biopsy specimens from true folds undergoing diagnostic laryngomicroscopy were prepared for...
To assess the susceptibility of salivary stones to bacterial biofilm formation, which may be involved in development gland infection, and investigate a relation between microbiological aspects patient characteristics. This prospective study comprises 54 patients with sialolithiasis attended Helsinki University Hospital during 2014–2016. A total 55 were removed, studied for formation using fluorescence microscopy sonication. The isolated organisms quantified identified matrix-assisted laser...
Objective: To investigate the microbiota of healthy external auditory canal (EAC) culture-independently and to evaluate usefulness swabbing method in collecting EAC samples. Study Design: Cohort study. Patients: Fifty asymptomatic working-age volunteers. Intervention: Samples were harvested with DNA-free swabs from volunteers’ EACs. Main Outcome Measures: Amplicon sequencing 16S rRNA gene was used characterize microbial communities Results: The is feasible for sample collection. analyzed 41...
Abstract Our aim was to validate a Finnish version of the Eating Assessment Tool (F-EAT-10) for clinical use and test its reliability validity in multicenter nationwide study. Normative data were acquired from 180 non-dysphagic participants (median age 57.0 years, 62.2% female). Dysphagia patients ( n = 117, median 69.7 53.0% female) referred fiberoptic endoscopic evaluation swallowing (FEES) completed F-EAT-10 before examination after 2 weeks. Patients underwent 100-ml water swallow (WST)...
Staphylococcus aureus is the most common cause of surgical site infections and its treatment challenging due to emergence multi-drug resistant strains such as methicillin-resistant S. (MRSA). Natural berry-derived compounds have shown antimicrobial potential, e.g., ellagitannins sanguiin H-6 lambertianin C, main phenolic in Rubus seeds, activity. The aim this study was evaluate effect C fractionated from cloudberry on MRSA growth, a biofilm development different growth media vitro vivo by...