A5029980341- Nanoplatforms for cancer theranostics
- Nanoparticle-Based Drug Delivery
- Photoacoustic and Ultrasonic Imaging
- Ultrasound and Hyperthermia Applications
- Radiation Therapy and Dosimetry
- Ultrasound Imaging and Elastography
- Advanced Fluorescence Microscopy Techniques
- Medical Imaging Techniques and Applications
- Lanthanide and Transition Metal Complexes
- Cell Image Analysis Techniques
- MRI in cancer diagnosis
- Anatomy and Medical Technology
- Receptor Mechanisms and Signaling
- Optical Coherence Tomography Applications
- Advanced Electron Microscopy Techniques and Applications
- Advanced Radiotherapy Techniques
- Melanoma and MAPK Pathways
- Characterization and Applications of Magnetic Nanoparticles
- Photodynamic Therapy Research Studies
- Metabolism, Diabetes, and Cancer
- Phagocytosis and Immune Regulation
- Cancer, Hypoxia, and Metabolism
- 14-3-3 protein interactions
- HER2/EGFR in Cancer Research
- Advanced MRI Techniques and Applications
The University of Texas MD Anderson Cancer Center
2023
Center for Systems Biology
2016-2018
Harvard University
2016-2018
Massachusetts General Hospital
2016-2018
University Health Network
2013-2018
University of Toronto
2007-2018
Princess Margaret Cancer Centre
2013-2018
3M (United States)
2016
Maastricht University
2013
Ontario Institute for Cancer Research
2013
Tumor hypoxia is a negative prognostic factor in multiple cancers, due part to its role causing resistance radiotherapy. Hypoxia arises tumor regions distal blood vessels as oxygen consumed by more proximal cells. Reducing the rate of consumption therefore potential strategy reduce hypoxia. We hypothesized that anti-diabetic drug metformin, which reduces through inhibition mitochondrial complex I, would improve radiation response increasing oxygenation.Tumor was measured xenografts before...
Radiation therapy enhances nanotherapeutic drug delivery in a tumor-associated macrophage–dependent fashion.
The discovery of the enhanced permeability and retention (EPR) effect has resulted in development nanomedicines, including liposome-based formulations drugs, as cancer therapies. use liposomes substantial increases accumulation drugs solid tumors; yet, significant improvements therapeutic efficacy have yet to be achieved. Imaging tumor revealed that this poor or variable performance is part due heterogeneous inter-subject intra-tumoral liposome accumulation, which occurs a result an abnormal...
Nanomedicine drug delivery systems are capable of transporting significant payloads to solid tumors. However, only a modest increase in antitumor efficacy relative the standard care has been observed. In this study, we demonstrate that single dose radiation or mild hyperthermia can substantially improve tumor uptake and distribution nanotherapeutics, resulting improved treatment efficacy. The nanomedicine was driven by reduction interstitial fluid pressure (IFP) small perturbation...
In conventional confocal/multiphoton fluorescence microscopy, images are typically acquired under ideal settings and after extensive optimization of parameters for a given structure or feature, often resulting in information loss from other image attributes. To overcome the problem selective data display, we developed new method that extends imaging dynamic range optical microscopy improves signal-to-noise ratio. Here demonstrate how real-time sequential high facilitates automated...
In vivo and direct imaging of the murine spinal cord its vasculature using multimodal (optical acoustic) techniques could significantly advance preclinical studies cord. Such intrinsically high resolution complementary technologies provide a powerful means quantitatively monitoring changes in anatomy, structure, physiology function living over time after traumatic injury, onset disease, or therapeutic intervention. However, longitudinal intact rodent models has been challenging, requiring...
Herein we describe fluorescent derivatives of vemurafenib to probe therapeutic BRAF inhibition in live cells and vivo.The compounds were evaluated compared by determining target binding, mutant melanoma cell lines imaging.We show that vemurafenib-BODIPY is a superior imaging drug visualize the targets vivo non-resistant resistant tumors.
Abstract Liposome-based anticancer agents take advantage of the increased vascular permeability and transvascular pressure gradients for selective accumulation in tumors, a phenomenon known as enhanced retention(EPR) effect. The EPR effect has motivated clinical use nano-therapeutics, with mixed results on treatment outcome. High interstitial fluid (IFP) been shown to limit liposome drug delivery central tumour regions. Furthermore, high IFP is an independent prognostic biomarker efficacy...
Purpose: This paper presents the design of a micro‐CT guided small animal robotic needle positioning system. In order to simplify and maintain targeting error, novel implementation remote center motion is used in The system has been developed with objective achieving mean error <200 μm while maintaining high degree user friendliness. Methods: robot compact enough operate within 25 cm diameter bore. Small animals can be imaged an intervention performed without need transport from one...
The heterogeneous intra-tumoral accumulation of liposomes is a critical determinant their efficacy. Both the chaotic tumor microcirculation and elevated IFP are linked to distribution nanotechnology-based drug delivery systems such as liposomes. In present study, relationship between microcirculation, IFP, nanoparticles was investigated through in vivo experimentation. This accomplished by evaluation using dynamic contrast enhanced computed tomography (DCE-CT) measurement novel image-guided...
Abstract VisualSonics has recently developed a preclinical photoacoustic (PA) imaging system called the VevoLAZR that combines sensitivity of optical and high resolution micro-ultrasound. The incorporates 40 MHz (centre frequency) ultrasound transducer linear array probe (LZ550) tuneable 680-970 nm nanosecond pulsed-laser. We used this to study in vivo changes tumor oxygen saturation haemoglobin density caused by exposure radiation therapy (RT). For this, DsRed-Me180 human cervical tumors...
The ability to perform quantitative haemodynamic measurements in the microcirculation using high frequency ultrasound and microbubbles is dependent on suppress tissue signals concentration of agent. While suppression has been accomplished by exploiting nonlinear properties microbubbles, it still unclear as optimal dose facilitate flow studies. A response study contrast Agent MicroMarker was performed renal cortex a mouse get an indication range doses. transmit pulse 30 MHz, 20% bandwidth...
The flexibility and sophistication of modern radiotherapy treatment planning delivery methods have advanced techniques to improve the therapeutic ratio. Contemporary dose optimization calculation algorithms facilitate plans which closely conform three-dimensional distribution target, with beam shaping devices image guided field targeting ensuring fidelity accuracy delivery. Ultimately, conformity is limited by maximum deliverable gradient; shallow gradients challenge deliver a tumoricidal...
The goal of this project was to determine the utility microbubble contrast agents in relation mouse imaging with high frequency ultrasound. ability perform quantitative haemodynamic measurements using ultrasound and microbubbles is dependent on behaviour agent vivo. objective study investigate acoustic response kinetic clinical Definitytrade mice exposed 30 MHz In vitro experiments showed that behaved predominantly as linear scatterers for pressures < 300 kPa signal loss after 600 pulses...
The purpose of this preliminary study was to quantify the destruction a microbubble contrast agent (Definity TM , 0.01 % volume fraction) by 20 or 25 MHz ultrasound under varying conditions exposure, including peak negative pressure (0.3 2.1 MPa), frequency (20 MHz), number cycles (NC= 1, 5, 10, 15 cycles), flow rate (6 12 mm/s) and frame (FR= 31, 47, 79 110 Hz). Experiments were performed in 800 µm diameter phantom, using two Vevo770 scanners (VisualSonics Inc.) equipped with an RMV710...
Purpose: To non-invasively quantify the interstitial volume fraction (IVF), vascular (VVF), and fluid pressure (IFP) in solid tumours. These properties have been shown to influence delivery of micro- macro-molecular weight drugs predict for radiation therapy outcome. Method Materials: A (MW) CT Liposome contrast agent was injected into orthotopic cervix tumour bearing mice (N=4) followed by injection a micro-MW iohexol agent. In-vivo kinetics two agents were measured using micro computed...