A5083977526- Cancer Immunotherapy and Biomarkers
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Digestive system and related health
- Atherosclerosis and Cardiovascular Diseases
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Gut microbiota and health
- Immune responses and vaccinations
Cold Spring Harbor Laboratory
2020-2023
Tumor-draining lymph nodes (TDLNs) are important for tumor antigen–specific T cell generation and effective anticancer immune responses. However, TDLNs often the primary site of metastasis, causing suppression worse outcomes. Through cross-species single-cell RNA-Seq analysis, we identified features defining cancer heterogeneity, plasticity, evasion during breast progression node metastasis (LNM). A subset cells in exhibited elevated MHC class II (MHC-II) gene expression both mice humans....
Abstract Tumor-draining lymph nodes are critical sites for generating tumor antigen-specific T cells and associated with durable immune responses. However, often the first site of metastasis node metastases portend worse outcomes. Through cross species single cell gene expression analysis breast cancer progression to nodes, we uncovered features that define heterogeneity, plasticity, evasion cells. Notably, a subpopulation metastatic in were marked by high levels MHC class II (MHC-II) both...
Abstract Immune surveillance of the intestinal epithelium is regulated by reciprocal interactions between epithelial cells, immune system and microbiome. This highly specialized compartment maintains tolerance against dietary products commensal bacteria while protecting pathogens, cancer risk tissue damage. While recent studies identified epigenetic mechanisms that orchestrate self-renewal differentiation cells (IECs), little known about govern – how they influence regeneration. Here we...
Abstract Little is known about how interactions between diet, immune recognition, and intestinal stem cells (ISCs) impact the early steps of tumorigenesis. Here, we show that a high fat diet (HFD) reduces expression major histocompatibility complex II (MHC-II) genes in ISCs. This decline ISC MHC-II HFD correlates with an altered microbiome composition recapitulated antibiotic treated germ-free mice on control diet. Mechanistically, pattern recognition receptor IFNg signaling regulate...
Summary Tumor-draining lymph nodes are critical sites for generating tumor antigen-specific T cells and associated with durable immune responses. However, often the first site of metastasis node metastases portend worse outcomes. Through cross-species single cell gene expression analysis breast cancer progression to nodes, we uncovered features that define heterogeneity, plasticity, evasion cells. Notably, a subpopulation metastatic in were marked by high levels MHC class II (MHC-II) both...