A5075963647- RNA Interference and Gene Delivery
- S100 Proteins and Annexins
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Cancer Immunotherapy and Biomarkers
- Protein purification and stability
- Neonatal Respiratory Health Research
- Immune Response and Inflammation
- Enzyme Structure and Function
- Cell Adhesion Molecules Research
- Bacteriophages and microbial interactions
- Protease and Inhibitor Mechanisms
- Protein Structure and Dynamics
- Immune Cell Function and Interaction
- Peptidase Inhibition and Analysis
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- Viral Infectious Diseases and Gene Expression in Insects
- CAR-T cell therapy research
- Bacterial Genetics and Biotechnology
- RNA modifications and cancer
- Antimicrobial Peptides and Activities
Okayama University
2016-2025
Nagasaki University Hospital
2019
Hiroshima University Hospital
2019
Kawasaki Medical School
2019
The University of Tokyo
2019
Nagasaki Prefectural Shimabara High School
2019
Nagasaki University
2019
Faculty (United Kingdom)
2005-2009
Engineering (Italy)
2005-2009
Nippon Shokubai (Japan)
2005-2008
There is a rapid global rise in obesity, and the link between obesity diabetes remains somewhat obscure. We identified an adipocytokine, designated as visceral adipose tissue-derived serpin (vaspin), which member of serine protease inhibitor family. Vaspin cDNA was isolated by from white tissues (WATs) Otsuka Long-Evans Tokushima fatty (OLETF) rat, animal model abdominal with type 2 diabetes. Rat, mouse, human vaspins are made up 392, 394, 395 amino acids, respectively; exhibit ≈40% homology...
Accumulating evidence indicates that dysfunction of mitochondria is a common feature Parkinson disease. Functional loss familial disease-linked gene, BRPK/PINK1 (PINK1), results in deterioration mitochondrial functions and eventual neuronal cell death. A chaperone protein has been shown to be substrate PINK1 kinase activity. In this study, we demonstrated another action point the cytoplasm. Phosphorylation Akt at Ser-473 was enhanced by overexpression PINK1, activation crucial for protection...
The tissue-specific expression of five human pancreatic-type RNases and RNase inhibitor was analyzed by Northern hybridization against poly(A)+RNA prepared from 16 normal tissues. widespread 1 observed in almost all the 4 angiogenin showed a similar distribution abundantly present liver. This suggested identity cell types producing these two molecules. However, no relativity appeared to be between vascularization tissues expression. A narrow range eosinophil-derived neurotoxin gene observed....
We previously revealed a novel signal pathway involving S100A11 for inhibition of the growth normal human keratinocytes (NHK) caused by high Ca(++) or transforming factor beta. Exposure to either agent resulted in transfer nuclei, where it induced p21(WAF1). In contrast, has been shown be overexpressed many cancers. To address this apparent discrepancy, we analyzed possible new functions S100A11, and provide herein evidence that 1) is actively secreted NHK; 2) extracellular acts on NHK...
For expression of genes in mammalian cells, various vectors have been developed using promoters including CMV, EF-1α, and CAG widely used. However, such sometimes fail to attain sufficient levels depending on the nature cargo and/or host cell types. In present study, we aimed develop a potent promoter system that enables high ubiquitously many different We found insertion an additional downstream gene greatly enhanced levels. Among constructs tested, C-TSC cassette (C: CMV-RU5′ located...
Metastatic breast cancer is the leading cause of cancer-associated death in women. The progression this fatal disease associated with inflammatory responses that promote cell growth and dissemination, eventually to a reduction overall survival. However, mechanism(s) inflammation-boosted remains unclear. In study, we found for first time an extracellular cytokine, S100A8/A9, accelerates metastasis upon binding surface receptor, melanoma adhesion molecule (MCAM). Our molecular analyses...
Since metastasis accounts for the majority of cancer-associated deaths, studies on mechanisms are needed to establish innovative strategies cancer treatment. We previously reported that melanoma cell adhesion molecule (MCAM) functions as a critical receptor S100A8/A9, and binding S100A8/A9 MCAM results in migration cells lung tissue. However, role original skin lesion is still not clear. In this study, we aimed determine importance S100A8/A9-MCAM axis dissemination early step metastasis....
Background Our earlier research revealed that the secreted lysyl oxidase-like 4 (LOXL4) is highly elevated in triple-negative breast cancer (TNBC) acts as a catalyst to lock annexin A2 on cell membrane surface, which accelerates invasive outgrowth of through binding integrin-β1 surface. However, whether this machinery subject LOXL4-mediated intrusive regulation remains uncertain. Methods Cell invasion was assessed using transwell-based assay, protein–protein interactions by an...
Abstract The production of disulfide‐containing recombinant proteins often requires refolding inclusion bodies before purification. A pre‐refolding purification step is crucial for effective because impurities in the interfere with and subsequent This study presents a new procedure using reversible S ‐cationization technique protein solubilization by reversed‐phase high performance liquid chromatography. pre‐folding improves yield effectively removing inhibitors from contaminates bacterial...
Carboxyl groups of bovine RNase A were amidated with ethylenediamine (to convert negative charges carboxylate anions to positive ones), 2-aminoethanol eliminate charges), and taurine keep respectively, by a carbodiimide reaction. Human 1 was also modified ethylenediamine. Surprisingly, the RNases all cytotoxic toward 3T3-SV-40 cells despite their decreased ribonucleolytic activity. However, enzymatic activity not completely eliminated presence excess cytosolic inhibitor (RI). As for native...
The metastatic dissemination of cancer cells to remote areas the body is most problematic aspect in patients. Among cancers, melanomas are notoriously difficult treat due their significantly high potential even during early stages. Hence, establishment advanced therapeutic approaches regulate metastasis required overcome melanoma disease. An accumulating mass evidence has indicated a critical role extracellular S100A8/A9 distant metastasis. Lung induced by from organs and it attracts these...
IntroductionProgrammed cell death-1 (PD-1) inhibitors effectively treat NSCLC and prolong survival. Robust biomarkers for predicting clinical benefits of good response long survival with anti–PD-1 therapy have yet to be identified; therefore, predictive are needed select patients benefits.MethodsWe conducted a prospective study explore whether serum antibody against NY-ESO-1 and/or XAGE1 cancer-testis antigens predicted primarily secondarily NSCLC. The was detected by enzyme-linked...
The REIC/Dkk-3 gene has been reported to be a tumor suppressor and the expression is significantly down-regulated in broad range of cancer cell types. protein secretory, but physiological function remains unclear. This study demonstrated that recombinant induced differentiation human CD14+ monocytes into novel type (REIC/Dkk-3Mo). REIC/Dkk-3Mo resembles immature dendritic cells generated with IL-4 GM-CSF. Both these populations exhibit similar proportions CD11c+, CD40+, CD86+ HLA-DR+...
The receptor for advanced glycation end products (RAGE) is involved in the pathogenesis of many inflammatory, degenerative, and hyperproliferative diseases, including cancer. Previously, we revealed mechanisms downstream signaling from ligand-activated RAGE, which recruits TIRAP/MyD88. Here, showed that DNAX-activating protein 10 (DAP10), a transmembrane adaptor protein, also binds to RAGE. By artificial oligomerization RAGE alone or RAGE-DAP10, found RAGE-DAP10 heterodimer formation...
Compiling evidence indicates an unusual role of extracellular S100A8/A9 in cancer metastasis. secreted from either cells or normal including epithelial and inflammatory stimulates through sensor receptors autocrine paracrine manner, leading to cell metastatic progression. We previously reported a novel receptor, neuroplastin-β (NPTNβ), which plays critical atopic dermatitis when it is highly activated keratinocytes by excess amount the skin lesion. Interestingly, our expression profiling...
S100A11, a member of the S100 family proteins, is actively secreted from pancreatic ductal adenocarcinoma (PDAC) cells. However, role extracellular S100A11 in PDAC progression remains unclear. In present study, we investigated crosstalking between cells and surrounding fibroblasts progression. An abundant cancer stimulated neighboring through receptor for advanced glycation end products (RAGE) upon binding was followed by not only an enhanced cell motility vitro but also increased number...
A protein crystal lattice consists of surface contact regions, where the interactions specific groups play a key role in stabilizing regular arrangement molecules. In an attempt to control incorporation lattice, leucine zipper-like hydrophobic interface (comprising four residues) was introduced into helical region (helix 2) human pancreatic ribonuclease 1 (RNase 1) that predicted form suitable crystallization interface. Although wild-type RNase has not yet been reported, mutant having...
We previously identified novel S100A8/A9 receptors, extracellular matrix metalloproteinase inducer (EMMPRIN), melanoma cell adhesion molecule (MCAM), activated leukocyte (ALCAM), and neuroplastin (NPTN) , that are critically involved in S100A8/A9-mediated cancer metastasis inflammation when expressed at high levels. However, little is known about the presence of any cancer-specific mechanism(s) modifies these further inducing upregulation protein levels without transcriptional regulation....
Gene expression systems with various promoters, including the cytomegalovirus (CMV) promoter, have been developed to increase gene in a variety of normal and cancer cells. In particular, clinical trials therapy, more efficient robust system is required achieve sufficient therapeutic outcomes. By inserting triple translational enhancer sequences human telomerase reverse transcriptase (hTERT), Simian virus 40 (SV40) CMV downstream sequence BGH polyA, we were able develop novel that...
Abstract Many extracellular globular proteins have evolved to possess disulphide bonds in their native conformations, which aids thermodynamic stabilisation. However, bond breakage by heating leads irreversible protein denaturation through disulphide-thiol exchange reactions. In this study, we demonstrate that methanethiosulphonate (MTS) specifically suppresses the heat-induced reaction, thus improving heat-resistance of proteins. presence MTS, small contain disulphides can spontaneously...
The fertile stroma in pancreatic ductal adenocarcinomas (PDACs) has been suspected to greatly contribute PDAC progression. Since the main cell constituents of are fibroblasts, there is crosstalking(s) between cells and surrounding fibroblasts stroma, which induces a fibroblast proliferation burst. We have reported that several malignant cancer including secrete pronounced level S100A11, turn stimulates via receptor for advanced glycation end products (RAGE) an autocrine manner. Owing RAGE +...