A5088047307- S100 Proteins and Annexins
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Immune Response and Inflammation
- Cancer Research and Treatments
- Neonatal Respiratory Health Research
- Protease and Inhibitor Mechanisms
- Cell Adhesion Molecules Research
- Advanced Glycation End Products research
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced biosensing and bioanalysis techniques
- Parkinson's Disease Mechanisms and Treatments
- CRISPR and Genetic Engineering
- Calcium signaling and nucleotide metabolism
- Medical Imaging Techniques and Applications
- Cancer-related gene regulation
- Sirtuins and Resveratrol in Medicine
- Antimicrobial Peptides and Activities
- Autophagy in Disease and Therapy
- Ubiquitin and proteasome pathways
- Biomarkers in Disease Mechanisms
- Fungal Biology and Applications
- Wnt/β-catenin signaling in development and cancer
- Cancer-related Molecular Pathways
- Cell death mechanisms and regulation
Okayama University
2015-2025
Institute of Cell Biology
2014
Okayama Prefecture
2009
Nippon Shokubai (Japan)
2006-2008
Shinshu University
2006
Ōtani University
2006
Hiroshima University
1990
The receptor for advanced glycation end products (RAGE) is thought to be involved in the pathogenesis of a broad range inflammatory, degenerative and hyperproliferative diseases. It binds diverse ligands activates multiple intracellular signaling pathways. Despite these pivotal functions, molecular events just downstream ligand-activated RAGE have been surprisingly unknown. Here we show that cytoplasmic domain phosphorylated at Ser391 by PKCζ upon binding ligands. TIRAP MyD88, which are...
Mutations of the PTEN-induced putative kinase 1 (PINK1) gene are a cause autosomal recessive forms Parkinson's disease. Recent studies have revealed that PINK1 is an essential factor for controlling mitochondrial quality, and it protects cells from oxidative stresses. Although there has been considerable progress in elucidation various aspects protein regulation such as activation, stability degradation, transcriptional mRNA under stress conditions remains unclear. In this study, we found...
Accumulating evidence indicates that dysfunction of mitochondria is a common feature Parkinson disease. Functional loss familial disease-linked gene, BRPK/PINK1 (PINK1), results in deterioration mitochondrial functions and eventual neuronal cell death. A chaperone protein has been shown to be substrate PINK1 kinase activity. In this study, we demonstrated another action point the cytoplasm. Phosphorylation Akt at Ser-473 was enhanced by overexpression PINK1, activation crucial for protection...
Mutations in PTEN-induced putative kinase 1 (PINK1) or parkin cause autosomal recessive forms of Parkinson's disease. Recent work suggests that loss mitochondrial membrane potential stabilizes PINK1 and accumulated recruits from the cytoplasm to mitochondria for elimination depolarized mitochondria, which is known as mitophagy. In this study, we find a complex with sterile α TIR motif containing (SARM1) tumor necrosis factor receptor–associated 6 (TRAF6), important import outer stabilization...
We previously revealed a novel signal pathway involving S100A11 for inhibition of the growth normal human keratinocytes (NHK) caused by high Ca(++) or transforming factor beta. Exposure to either agent resulted in transfer nuclei, where it induced p21(WAF1). In contrast, has been shown be overexpressed many cancers. To address this apparent discrepancy, we analyzed possible new functions S100A11, and provide herein evidence that 1) is actively secreted NHK; 2) extracellular acts on NHK...
For expression of genes in mammalian cells, various vectors have been developed using promoters including CMV, EF-1α, and CAG widely used. However, such sometimes fail to attain sufficient levels depending on the nature cargo and/or host cell types. In present study, we aimed develop a potent promoter system that enables high ubiquitously many different We found insertion an additional downstream gene greatly enhanced levels. Among constructs tested, C-TSC cassette (C: CMV-RU5′ located...
Metastatic breast cancer is the leading cause of cancer-associated death in women. The progression this fatal disease associated with inflammatory responses that promote cell growth and dissemination, eventually to a reduction overall survival. However, mechanism(s) inflammation-boosted remains unclear. In study, we found for first time an extracellular cytokine, S100A8/A9, accelerates metastasis upon binding surface receptor, melanoma adhesion molecule (MCAM). Our molecular analyses...
Background Our earlier research revealed that the secreted lysyl oxidase-like 4 (LOXL4) is highly elevated in triple-negative breast cancer (TNBC) acts as a catalyst to lock annexin A2 on cell membrane surface, which accelerates invasive outgrowth of through binding integrin-β1 surface. However, whether this machinery subject LOXL4-mediated intrusive regulation remains uncertain. Methods Cell invasion was assessed using transwell-based assay, protein–protein interactions by an...
We previously showed that the tumor suppressor gene REIC/Dkk-3, when overexpressed by an adenovirus (Ad-REIC), exhibited a dramatic therapeutic effect on human cancers through mechanism triggered endoplasmic reticulum stress. Adenovirus vectors show no target cell specificity and thus may elicit unfavorable side effects infection of normal cells even upon intra-tumoral injection. In this study, we examined possible Ad-REIC cells. found fibroblasts (NHF) did not cause apoptosis but induced...
The metastatic dissemination of cancer cells to remote areas the body is most problematic aspect in patients. Among cancers, melanomas are notoriously difficult treat due their significantly high potential even during early stages. Hence, establishment advanced therapeutic approaches regulate metastasis required overcome melanoma disease. An accumulating mass evidence has indicated a critical role extracellular S100A8/A9 distant metastasis. Lung induced by from organs and it attracts these...
Since metastasis accounts for the majority of cancer-associated deaths, studies on mechanisms are needed to establish innovative strategies cancer treatment. We previously reported that melanoma cell adhesion molecule (MCAM) functions as a critical receptor S100A8/A9, and binding S100A8/A9 MCAM results in migration cells lung tissue. However, role original skin lesion is still not clear. In this study, we aimed determine importance S100A8/A9-MCAM axis dissemination early step metastasis....
The receptor for advanced glycation end products (RAGE) is involved in the pathogenesis of many inflammatory, degenerative, and hyperproliferative diseases, including cancer. Previously, we revealed mechanisms downstream signaling from ligand-activated RAGE, which recruits TIRAP/MyD88. Here, showed that DNAX-activating protein 10 (DAP10), a transmembrane adaptor protein, also binds to RAGE. By artificial oligomerization RAGE alone or RAGE-DAP10, found RAGE-DAP10 heterodimer formation...
Angiogenesis is essential for tumor development and metastasis. Among several angiogenic factors, vascular endothelial growth factor receptor (VEGF) important tumor-derived angiogenesis commonly overexpressed in solid tumors. Thus, many antitumor strategies targeting VEGF have been developed to inhibit cancer angiogenesis, offering insights into the successful treatment of cancers. However, there are a number issues such as harmful effects on normal vascularity clinical trials. Taking this...
Compiling evidence indicates an unusual role of extracellular S100A8/A9 in cancer metastasis. secreted from either cells or normal including epithelial and inflammatory stimulates through sensor receptors autocrine paracrine manner, leading to cell metastatic progression. We previously reported a novel receptor, neuroplastin-β (NPTNβ), which plays critical atopic dermatitis when it is highly activated keratinocytes by excess amount the skin lesion. Interestingly, our expression profiling...
S100A11, a member of the S100 family proteins, is actively secreted from pancreatic ductal adenocarcinoma (PDAC) cells. However, role extracellular S100A11 in PDAC progression remains unclear. In present study, we investigated crosstalking between cells and surrounding fibroblasts progression. An abundant cancer stimulated neighboring through receptor for advanced glycation end products (RAGE) upon binding was followed by not only an enhanced cell motility vitro but also increased number...
Herbal medicines and their bioactive compounds are increasingly being recognized as useful drugs for cancer treatments. The parasitic fungus Cordyceps militaris is an attractive anticancer herbal since it shows very powerful activity due to its phytocompound cordycepin. We previously discovered reported that a high amount of xylitol present in extract, unexpectedly showed cancer-selective manner. thus hypothesized could become supplement help prevent various cancers, if we can clarify the...
Osteosarcoma is the most common malignant tumor of bone in childhood and adolescence. Despite intensive research for new therapies, outcome patients with metastasis remains extremely poor. S100 proteins are involved proliferation, cell cycle progression numerous tumors, including osteosarcoma. In present study, we identified S100A7 as a candidate to promote migration osteosarcoma cells. promoted invasion cells assayed vitro. An vitro pull-down assay revealed binding recombinant protein its...
We previously identified novel S100A8/A9 receptors, extracellular matrix metalloproteinase inducer (EMMPRIN), melanoma cell adhesion molecule (MCAM), activated leukocyte (ALCAM), and neuroplastin (NPTN) , that are critically involved in S100A8/A9-mediated cancer metastasis inflammation when expressed at high levels. However, little is known about the presence of any cancer-specific mechanism(s) modifies these further inducing upregulation protein levels without transcriptional regulation....