A5070911300- Cholesterol and Lipid Metabolism
- Antioxidant Activity and Oxidative Stress
- Liver Disease Diagnosis and Treatment
- Free Radicals and Antioxidants
- Vitamin C and Antioxidants Research
- Atherosclerosis and Cardiovascular Diseases
- Eicosanoids and Hypertension Pharmacology
- Antiplatelet Therapy and Cardiovascular Diseases
- Hormonal Regulation and Hypertension
- Steroid Chemistry and Biochemistry
- Sphingolipid Metabolism and Signaling
- Nitric Oxide and Endothelin Effects
- Liver Disease and Transplantation
- Electron Spin Resonance Studies
- Fatty Acid Research and Health
- Lipoproteins and Cardiovascular Health
- Peroxisome Proliferator-Activated Receptors
- Cancer, Lipids, and Metabolism
- Adipose Tissue and Metabolism
- Inflammatory mediators and NSAID effects
- Adipokines, Inflammation, and Metabolic Diseases
- Cardiac Imaging and Diagnostics
- Receptor Mechanisms and Signaling
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Estrogen and related hormone effects
Sapienza University of Rome
2015-2024
Université de Toulouse
2023
Inserm
2023
Centre National de la Recherche Scientifique
2023
Istituto Ortopedico Rizzoli
2018
Università Campus Bio-Medico
2018
Istituti di Ricovero e Cura a Carattere Scientifico
2011-2016
University of Rome Tor Vergata
2000-2016
Magna Graecia University
2016
University of Turin
2016
Alzheimer's disease (AD) is a gradually debilitating that leads to dementia. The molecular mechanisms underlying AD are still not clear, and at present no reliable biomarkers available for the early diagnosis. In last several years, together with oxidative stress neuroinflammation, altered cholesterol metabolism in brain has become increasingly implicated progression. A significant body of evidence indicates oxidized cholesterol, form oxysterols, one main triggers AD. oxysterols potentially...
F2-Isoprostanes are prostaglandin (PG) isomers formed in situ cell membranes by peroxidation of arachidonic acid. 8-epi PGF2alpha and IPF2alpha-I F2-isoprostanes produced humans which circulate plasma excreted urine. Measurement may offer a sensitive, specific, noninvasive method for measuring oxidant stress clinical settings where reactive oxygen species putatively involved. We determined whether isoprostanes were present human atherosclerotic lesions, lipid is thought to occur vivo. ranged...
Isoprostanes are prostaglandin isomers produced from arachidonic acid by a free radical-catalyzed mechanism. Urinary excretion of 8- iso -prostaglandin F 2α , an isomer the PGG/H synthase (cyclooxygenase or COX) enzyme product, (PGF ), has exhibited promise as index oxidant stress in vivo. We have developed quantitative method to measure isoprostane -I, (IPF -I) class I (8- -PGF is IV), using gas chromatography/mass spectrometry. IPF -I severalfold abundant human urine iso- PGF with mean...
Significance Cholesterol and its transformation into cholesterol-5,6-epoxides (5,6-EC) was long suspected as contributing to breast cancer (BC) pathogenesis, before it found that 5,6-EC metabolism controls BC development is deregulated in cancers. Herein, we studied tumor cells human samples how deregulation promotes progression. We have discovered a pathway BCs producing an oncometabolite derived from 5,6-EC, through the action of cortisol-inactivating enzyme, identified glucocorticoid...
Platelet activation has been demonstrated in experimental and clinical models of ischemia-reperfusion, but the underlying mechanism is still unclear. We mimicked ischemia-reperfusion model vitro by exposing platelets to anoxia-reoxygenation (A-R) evaluated role oxygen free radicals (OFRs), which are usually produced during reperfusion phase, inducing platelet activation.Human were exposed 15 30 minutes anoxia then reoxygenated. Compared with control kept atmospheric conditions, A-R showed...
Global sterol analysis is challenging owing to the extreme diversity of natural products, tendency cholesterol dominate in abundance over all other sterols, and structural lack a strong chromophore or readily ionized functional group. We developed method overcome these challenges by using different isotope-labeled versions Girard P reagent (GP) as quantitative charge-tags for LC-MS sterols including oxysterols.Sterols/oxysterols plasma were extracted ethanol containing deuterated internal...
Macrophages consist of two main subsets: the proinflammatory M1 subset and anti-inflammatory M2 one. 7-oxo-cholesterol, most abundant cholesterol autoxidation product within atherosclerotic plaque, is able to skew M1/M2 balance towards a profile. In present study, we explored ability polyphenolic compound resveratrol counteract 7-oxo-cholesterol-triggered signaling in macrophages. Resveratrol-pretreated human monocyte-derived macrophages were challenged with 7-oxo-cholesterol analyzed for...
The human brain contains approximately 25% of the body's cholesterol. is separated from circulation by blood barrier. While cholesterol will not passes this barrier, oxygenated forms can cross Here measuring difference in oxysterol content plasma jugular vein and a forearm mass spectrometry (MS) we were able to determine flux more than 20 metabolites between circulation. We confirm that 24S-hydroxycholesterol exported at rate about 2–3 mg/24 h. Gas chromatography (GC)–MS data shows...
Consumption of extra virgin olive oil (EVOO), a major component the Mediterranean diet, has been associated with reduced incidence Alzheimer's disease (AD). However, mechanisms involved in this protective action remain to be fully elucidated.Herein, we investigated effect daily consumption EVOO on AD-like phenotype mouse mode plaques and tangles.Triple transgenic mice (3xTg) received either regular chow or diet supplemented starting at 6 months age for months, then assessed neuropathology...
The complete mechanism accounting for the progression from simple steatosis to steatohepatitis in nonalcoholic fatty liver disease (NAFLD) has not been elucidated. Lipotoxicity refers cellular injury caused by hepatic free acids (FFAs) and cholesterol accumulation. Excess autoxidizes oxysterols during oxidative stress conditions. We hypothesize that interaction of FAs derivatives may primarily impair mitochondrial function affect biogenesis adaptation NAFLD progression. demonstrated...
Iron is an important modulator of lipid peroxidation, and its levels have been associated with the progression atherosclerosis. Little known about possibility that this metal, when released from tissue stores, may modulate reactivity blood cell components, in particular platelets. Therefore, we investigated a possible link between iron, oxygen free radical formation, platelet function.Human whole was stimulated collagen 2 micrograms/mL, irreversible aggregation thromboxane (Tx)B2 formation...