A5053612130- Antibiotic Resistance in Bacteria
- Fungal and yeast genetics research
- Immune Response and Inflammation
- Vibrio bacteria research studies
- Immune cells in cancer
- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- Gut microbiota and health
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- Probiotics and Fermented Foods
- interferon and immune responses
- Tuberculosis Research and Epidemiology
- Inflammasome and immune disorders
- vaccines and immunoinformatics approaches
- Fermentation and Sensory Analysis
- Antimicrobial Peptides and Activities
- Bacterial biofilms and quorum sensing
- Biofuel production and bioconversion
- Hepatitis B Virus Studies
- Inhalation and Respiratory Drug Delivery
- Single-cell and spatial transcriptomics
- Amoebic Infections and Treatments
- Machine Learning in Bioinformatics
- Amino Acid Enzymes and Metabolism
Queen's University Belfast
2016-2025
University of Minho
2011-2018
Zero to Three
2011
Research Article15 February 2017Open Access Source DataTransparent process A Klebsiella pneumoniae antibiotic resistance mechanism that subdues host defences and promotes virulence Timothy J Kidd orcid.org/0000-0002-6135-6364 Centre for Experimental Medicine, Queen's University Belfast, UK School of Chemistry Molecular Biosciences, The Queensland, Brisbane, Qld, Australia Child Health Centre, Search more papers by this author Grant Mills Joana Sá-Pessoa Amy Dumigan Christian G Frank José L...
Klebsiella pneumoniae is recognized as an urgent threat to human health due the increasing isolation of multidrug resistant strains. Hypervirulent strains are a major concern their ability cause life-threating infections in healthy hosts. The type VI secretion system (T6SS) widely implicated microbial antagonism, and it mediates interactions with host eukaryotic cells some cases. In silico search for genes orthologous T6SS component effector across 700 K. genomes shows extensive diversity...
Klebsiella pneumoniae is a leading cause of nosocomial and community acquired infections, making K. the pathogen that associated with second largest number deaths attributed to any antibiotic resistant infection. colonizes nasopharynx gastrointestinal tract in an asymptomatic manner without dissemination other tissues. Importantly, colonization requisite for Our understanding still based on interrogating mouse models which animals are pretreated antibiotics disturb resistance imposed by gut...
Abstract Bacteria can inhibit the growth of other bacteria by injecting effectors using a type VI secretion system (T6SS). T6SS also be injected into eukaryotic cells to facilitate bacterial survival, often targeting cytoskeleton. Here, we show that trans-kingdom antimicrobial effector VgrG4 from Klebsiella pneumoniae triggers fragmentation mitochondrial network. colocalizes with endoplasmic reticulum (ER) protein mitofusin 2. induces transfer Ca 2+ ER mitochondria, activating Drp1 (a...
We aimed to manipulate the metabolism of Saccharomyces cerevisiae produce lactic acid and search for potential influence transport across plasma membrane in this process. W303-1A is able use l-lactic but its production our laboratory has not previously been detected. When l-LDH gene from Lactobacillus casei was expressed S. isogenic mutants jen1∆, ady2∆ jen1∆ ady2∆, all strains were acid, higher titres achieved mutant strains. In constitutively expressing both LDH JEN1 or ADY2, a external...
In the present paper we describe a new carboxylic acid transporter in Escherichia coli encoded by gene yaaH. contrast to what had been described for other YaaH family members, E. is highly specific acetic (a monocarboxylate) and succinic dicarboxylate), with affinity constants at pH 6.0 of 1.24±0.13 mM 1.18±0.10 acid. glucose-grown cells ΔyaaH mutant compromised uptake both labelled acids. YaaH, together ActP, previously as an acetate transporter, affect use sole carbon energy source. Both...
Many bacterial pathogens antagonize host defense responses by translocating effector proteins into cells. It remains an open question how those not encoding effectors counteract anti-bacterial immunity. Here, we show that Klebsiella pneumoniae exploits the evolutionary conserved innate protein SARM1 to regulate negatively MyD88- and TRIF-governed inflammation, activation of MAP kinases ERK JNK. is required for induction interleukin-10 (IL-10) fine-tuning p38-type I interferon (IFN) axis....
Acinetobacter baumannii causes a wide range of nosocomial infections. This pathogen is considered threat to human health due the increasingly frequent isolation multidrug-resistant strains.
Klebsiella pneumoniae has been singled out as an urgent threat to human health due the increasing isolation of strains resistant “last-line” antimicrobials, narrowing treatment options against infections. Unfortunately, at present, we cannot identify candidate compounds in late-stage development for multidrug-resistant infections; this pathogen is exemplary mismatch between unmet medical needs and current antimicrobial research pipeline. Furthermore, there still limited evidence on K....
In vivo single-cell approaches have transformed our understanding of the immune populations in tissues. Mass cytometry (CyTOF), that combines resolution mass spectrometry with ability to conduct multiplexed measurements cell molecules at single resolution, has enabled resolve diversity subsets, and their heterogeneous functionality. Here we assess feasibility taking CyTOF one step further immuno profile cells while tracking interactions bacteria, a method term Bac-CyTOF. We focus on pathogen...
Polymicrobial biofilms consisting of fungi and bacteria are frequently formed on endotracheal tubes may contribute to development ventilator associated pneumonia (VAP) in critically ill patients. This study aimed determine the role early Candida albicans supporting dual-species (dual-kingdom) biofilm formation with respiratory pathogens vitro , investigated effect targeted antifungal treatment bacterial cells within biofilms. Dual-species between C . three commonly VAP ( Pseudomonas...
SUMMARY Vasodilation is a crucial protective response to inflammation and infection. Endothelial cells control vasodilation through the bioavailability of eNOS-produced nitric oxide (NO), generation endothelium-dependent hyperpolarization (EDH). Here, we demonstrate that Klebsiella pneumoniae , one most prevalent blood stream infection pathogens, inhibits agonist-induced by blunting NO-dependent pathway attenuating EDH pathway. The type VI secretion system (T6SS) effector VgrG4 licences...
Previous mutational analysis of Jen1p, a Saccharomyces cerevisiae monocarboxylate/H⁺ symporter the Major Facilitator Superfamily, has suggested that consensus sequence ³⁷⁹NXX[S/T]HX[S/T]QD³⁸⁷ in transmembrane segment VII (TMS-VII) is part substrate translocation pathway. Here, we rationally design, analyse and show several novel mutations TMS-V TMS-XI directly modify Jen1p function. Among residues studied, F270 (TMS-V) Q498 (TMS-XI) are critical specificity determinants for distinction mono-...
The implementation of infection models that approximate human disease is essential to understand infections and for testing new therapies before they enter into clinical stages. Rodents are used in most preclinical studies, although the differences between mice humans have fueled conclusion murine studies unreliable predictors outcomes. In this study, we developed a whole-lung porcine model using ex vivo lung perfusion (EVLP) system established recondition lungs transplant. As proof...
PYHIN proteins AIM2 and IFI204 sense pathogen DNA, while other PYHINs have been shown to regulate host gene expression through as-yet unclear mechanisms. We characterize mouse IFI207, which we find is not involved in DNA sensing but rather required for cytokine promoter induction macrophages. IFI207 co-localizes with both active RNA polymerase II (RNA Pol II) IRF7 the nucleus enhances IRF7-dependent induction. Generation of Ifi207-/- mice shows no role autoimmunity. Rather, establishment a...
Sugar acids can be used as platform chemicals to generate primary building blocks of industrially relevant products. Microbial production these organic compounds at high yields requires the engineering enzymatic machinery and presence plasma membrane transporters able export them outside cells. In this study, several yeast carboxylic acid belonging Jen family were screened for transport biotechnologically sugar acids, namely gluconic, saccharic, mucic, xylaric xylonic acid, functionally...
<b>Background:</b> Colistin is a last-line agent for treating severe multidrug resistant <i>Klebsiella pneumoniae</i> (<i>Kp</i>) infections. Recent studies highlight the emergence of colistin <i>Kp</i> arising from inducible inactivation <i>mgrB</i> gene, negative PhoPQ signalling system regulator. <b>Objectives:</b> To determine molecular mechanisms resistance and its impact upon fitness virulence. <b>Methods:</b> We constructed deletion mutant. Mass spectrometry (MS) was used to identify...
ABSTRACT Klebsiella pneumoniae is recognized as an urgent threat to human health due the increasing isolation of multidrug resistant strains. Hypervirulent strains are a major concern their ability cause life-threating infections in healthy hosts. The type VI secretion system (T6SS) widely implicated microbial antagonism, and it mediates interactions with host eukaryotic cells some cases. In silico search for genes orthologous T6SS component effector across 700 K. genomes shows extensive...
ABSTRACT Klebsiella pneumoniae is a leading cause of nosocomial and community acquired infections, making K. the second pathogen associated with most deaths attributed to any antibiotic resistant infection. colonises nasopharynx gastrointestinal tract in an asymptomatic manner without dissemination other tissues; importantly colonisation requisite for Our understanding still based on interrogating mouse models which animals are pre-treated antibiotics disturb resistance imposed by gut...