A5059013074- Biosensors and Analytical Detection
- Antibiotics Pharmacokinetics and Efficacy
- Synthesis and Biological Evaluation
- Trace Elements in Health
- Analytical chemistry methods development
- Carbohydrate Chemistry and Synthesis
- Pharmaceutical and Antibiotic Environmental Impacts
- Advanced biosensing and bioanalysis techniques
- Pharmacological Effects and Assays
- Antibiotic Resistance in Bacteria
- Synthesis and Reactions of Organic Compounds
- Vanadium and Halogenation Chemistry
- Coccidia and coccidiosis research
- Insect and Pesticide Research
- Pesticide Residue Analysis and Safety
- Traditional Chinese Medicine Analysis
- Neurological Disease Mechanisms and Treatments
- Animal Nutrition and Physiology
- Amino Acid Enzymes and Metabolism
- RNA modifications and cancer
- Legume Nitrogen Fixing Symbiosis
- Fungal Plant Pathogen Control
- Polyamine Metabolism and Applications
- Soil Moisture and Remote Sensing
- Pneumonia and Respiratory Infections
Chinese Academy of Medical Sciences & Peking Union Medical College
2024-2025
Xiyuan Hospital
2024-2025
Qingdao University
2021-2023
Qingdao Agricultural University
2015-2023
Guangxi Medical University
2022
Wannan Medical College
2021
China Agricultural University
1997-2020
University of Florida
2015
Hefei University of Technology
2015
Beijing Haidian Hospital
2013
An antibody with broad specificity and principally depending on hapten structure size is a key reagent for developing class-selective immunoassay. In the present study, three new generic haptens of antibacterial synergists (ASGs) were proposed using trimethoprim as starting molecule. These contained carboxyl groups meta position trimethoxybenzene conjugating to protein, while, common moiety ASGs, i.e., diaminopyrimidine, was intentionally maximally exposed immune system in animals order...
Introduction Acute ischemic stroke (AIS) poses a significant risk to human health. Intravenous thrombolysis and mechanical thrombectomy are essential treatments for AIS, offering substantial benefits neurological recovery brain protection. However, their efficacy is often limited by stringent time constraints contraindications, restricting accessibility certain patient populations. Investigating novel therapeutic strategies is, therefore, crucial. Our team developed Xiongzhitongluo granules...
Molecular recognition between a receptor and ligand is fundamental event in bioanalytical assays, which guarantees the sensitivity specificity of an assay for detection target interest. An intensive understanding interaction mechanism could be useful desirable hapten design, directed antibody evolution vitro, improvement. To illustrate structural information on class-specific monoclonal antibodies (mAbs) dihydropteroate synthase (DHPS) against sulfonamides (SAs) we have previously prepared,...
The aim of this study was to compare the effect dietary supplementation with low dose porous and nano zinc oxide (ZnO) on weaning piglets, evaluate possibility using them as an alternative high regular ZnO. Piglets were randomly allocated into four treatment groups fed diets: (1) basal diet (NC), (2) NC+ 3000 mg/kg ZnO (PC), (3) NC + 500 (HiZ) (4) (ZNP). result showed that piglets in HiZ group had less diarrhea than ZNP (P < 0.05). Besides, there no significant difference between PC, terms...
Rapid determination of orbifloxacin residue in milk by an optimized fluorescence polarization immunoassay based on a heterogeneous fluorescent tracer.
The development of multianalyte immunoassays with an emphasis on food safety has attracted increasing interest, due to its high target throughput, short detection time, reduced sample consumption, and low overall cost. In this study, a superior polyclonal antibody (pAb) against sulfonamides (SAs) was raised by using bioconjugate bovine serum albumin rationally designed hapten 4-[(4-aminophenyl) sulfonyl-amino]-2-methoxybenzoic acid (SA10-X). results showed that the pAb could recognize 19 SAs...
Cadmium (Cd) is a toxic heavy metal that poses threat to the health of humans and animals. It can cause serious damage small intestine, which main absorption site Cd primary target organ after oral administration. Our previous study found zinc chelate hydroxy analogue methionine (Zn-HMTB), new type feed additive, decreased accumulation in liver kidneys. The aim this was investigate effect Zn-HMTB on Cd-induced toxicity intestine piglets. Twenty-four piglets (Landrace × Large White, 13.22 ±...
Three immunizing haptens, 6-(4-aminophenylsulfonamido)hexanoic acid (HS), 4-(4-(4-aminophenylsulfonamido)phenylsulfonamido)benzoic (SSS) and 4-(4-(4-aminophenylsulfonamido)phenyl)butanoic (SA10), were synthesized used as haptens to produce generic polyclonal antibodies against sulfonamides. A novel enzyme-linked immunosorbent immunoassay (ELISA) for the detection of sulfonamides was developed based on one site heterologous coating hapten derived from sulfadiazine (STZ), providing IC50 values...
Abstract A research model combining a disease and syndrome can provide new ideas for the treatment of ischemic stroke. In field traditional Chinese medicine, blood stasis toxin (BST) is considered an important seen in patients with stroke (IS). However, biological basis IS-BST currently not well understood. Therefore, this study aimed to explore mechanism syndrome. This divided into two parts: (1) establishment animal BST stroke; (2) use omics methods identify differentially expressed genes...
The optimum conditions of a direct competitive enzyme-linked immunosorbent assay (dcELISA) in regard to different monoclonal antibodies (MAbs), coating conditions, blocking and physicochemical factors the buffer were investigated develop broad-specific sensitive immunoassay for detection antibacterial synergists (ASGs) milk, eggs, chicken pork. Under optimized highly dcELISA ASGs was obtained based on 5C4 hapten-C-HRP, demonstrating 50% specific binding (IC50) five 0.208–9.24 ng mL−1 with...
Aristolochic acid I (AAI) is one of the nephrotoxic derivatives present in genera Aristolochia and Asarum .
Abstract Three fractions (I–III) were separated from crude oligosaccharides of lotus seeds by fast protein liquid chromatography with final purity 97.6, 96.3, and 96.8%, respectively. The identified as sucrose, raffinose, stachyose using TLC, HPLC charged aerosol detector (CAD), LC‐MS, methylation analysis. Subsequently sucrose raffinose family (RFOs) degree polymerization (DP) 3–5 (raffinose, stachyose, verbascose) have been quantified HPLC‐CAD for the first time. All calibration curves...
The compound, 4,4′-dinitrocarbanilide (DNC), is the marker residue of concern in edible tissues broilers fed with diets containing anticoccidial nicarbazin (NIC). In this study, 25 fluorescein-labeled DNC derivatives (tracers) are synthesized and characterized to develop a rapid fluorescence polarization immunoassay (FPIA) for detection chickens using monoclonal antibodies (mAbs). effect tracer structure on sensitivity FPIA investigated. Our results show that after optimization, half maximal...